GENETICS OF AGAMOUS ACTION IN ARABISOPSIS DEVELOPMENT

拟南芥发育中无性行为的遗传学

• 批准号: 3305101
• 负责人: ELLIOT M MEYEROWITZ
• 金额: $ 16.6万
• 依托单位: CALIFORNIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-04-01 至 1994-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3305101
• 关键词: DNA binding protein; angiosperms; antibody; cell cycle; developmental genetics; gene expression; gene interaction; genetic models; genetic regulation; genome; genotype; homeobox genes; immunological substance; in situ hybridization; laboratory rabbit; model design /development; molecular cloning; molecular genetics; monoclonal antibody; plant genetics; plant growth /development; regulatory gene; reporter genes; tissue mosaicism

The long term objective of the proposed experiments is the production, testing and refinement of a molecular and genetic model for pattern formation in flower development. This will be done by studying in depth the genetics and molecular genetics of a key homeotic gene in the development of flowers of the plant Arabidopsis thaliana. This is the AGAMOUS gene, whose phenotype is the homeotic conversion of the six stamens found in wild type flowers to six petals, and of the ovary normally found in the center of the flower into a whorl of sepals. In addition, the wild type AGAMOUS gene appears to be responsible for the regulation of the amount and pattern of cell division in several flower parts. Since plant development consists only of the two processes of regulated cell division and patterned cellular differentiation (unlike animals, in which cell migration and maternal information play major roles), understanding the activities of the AGAMOUS gene will be a major step in understanding pattern formation in plants. Since this process appears much simpler in plants than in animals, it is to be expected that many aspects of development in plants will not only be of interest in their own right, but also serve as models for the more complex developmental processes of animals. The AGAMOUS gene is already cloned, and its protein product shares substantial homology with a class of transcriptional regulators known in vertebrates and in baker's yeast. The human homologue is Serum Response Factor, which is a key transcriptional regulator of the human c-fos oncogene. This underlies the relatedness of the proposed studies to the health problems of birth defects, caused by abnormal embryonic pattern formation, and of carcinogenesis. The specific aims are to study the action of AGAMOUS and its interaction with a series of additional homeotic genes. Among the proposed experiments are the careful study of the place and time of AG action and expression, by in situ analysis of RNA, by antibody localization of the AG protein, and by expression of reporter genes. All of this will be done in wild type and various mutant backgrounds. In additional a series of experiments to spatially and temporally misexpress the gene are planned, as are genetic mosaic studies. Finally, a detailed study of the newly-discovered family of AG homologues in the Arabidopsis genome will be performed, to learn the activity of the many other plant genes now known to be related to AGAMOUS, and thus to human Serum Response Factor.

所提出的实验的长期目标是生产， 测试和完善的分子和遗传模型的模式 花发育中的形成。 这将通过深入研究 一个关键同源异型基因的遗传学和分子遗传学， 拟南芥花的发育。 这是 AGAMOUS基因，其表型为六个雄蕊的同源异型转化 发现在野生型花到六个花瓣，和子房通常发现 在花的中心形成一轮萼片。 此外，野生 AGAMOUS型基因似乎负责调节 花的几个部分细胞分裂的数量和模式。由于植物 发育只包括两个受调节的细胞分裂过程 和模式化的细胞分化（不像动物， 移民和产妇信息发挥主要作用），了解 AGAMOUS基因的活性将是了解 植物的模式形成。 由于这个过程在 植物比动物，这是可以预期的，许多方面的 植物的发展不仅对它们本身有利， 也可以作为更复杂的发展过程的模型， 动物 AGAMOUS基因已经克隆，其蛋白质产物与 与已知的一类转录调节因子基本同源， 在脊椎动物和面包酵母中。 人类同源物为血清反应 因子，这是一个关键的转录调控因子的人c-fos 癌基因 这说明了拟议研究与 出生缺陷的健康问题，由异常胚胎模式引起 形成和致癌作用。 具体目标是研究 AGAMOUS的作用及其与一系列额外的同源异形体的相互作用 基因. 在提议的实验中， 和AG作用和表达的时间，通过RNA的原位分析， AG蛋白的抗体定位，以及通过报告基因的表达 基因. 所有这些都将在野生型和各种突变型中进行 背景 在另外的一系列实验中， 暂时错误表达基因的计划，因为是遗传镶嵌研究。 最后，对新发现的AG同系物家族进行了详细研究， 将进行拟南芥基因组中，以了解 现在已知许多其他植物基因与AGAMOUS有关，因此， 人血清反应因子