BINDONG LIU, PHD, LAB STARTUP PACKAGE: HIV IN AFRICAN AMERICANS

BINDONG LIU，博士，实验室启动计划：非洲裔美国人中的艾滋病毒

• 批准号: 7724718
• 负责人: Bindong Liu
• 金额: $ 19.6万
• 依托单位: MEHARRY MEDICAL COLLEGE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7724718
• 关键词: Acquired Immunodeficiency Syndrome; Affect; African American; Alleles; Anti-Retroviral Agents; CD4 Positive T Lymphocytes; Codon Nucleotides; Computer Retrieval of Information on Scientific Projects Database; Condition; Cytidine Deaminase; DNA; Defect; Disease Progression; Exons; Funding; Genetic Variation; Grant; HIV; HIV-1; In Vitro; Institution; Nuclear Import; Population; Prevention; Protein Family; Race; Rate; Reporting; Research; Research Personnel; Resources; Source; Therapeutic; Time; United States National Institutes of Health; Variant; Viral Reverse Transcription; apolipoprotein B mRNA editing enzyme; ethnic minority population; improved; interest; polypeptide; racial and ethnic; transmission process; viral DNA

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Apolipoprotein B mRNA editing enzyme catalytic polypeptide-like 3G (Apobec3G) is a newly defined host anti-retroviral factor. It belongs to a family of proteins that have cytidine deaminase activity. It is commonly believed that Apobec 3G restricts HIV-1 replication by inducing lethal G to A hypermutations in the newly synthesized viral DNA. It has also been observed that Apobec3G causes viral reverse transcription (RT), DNA nuclear import and integration of multiple steps of defects during HIV-1 replication. There are still some major gaps between the hypermutations and the multiple steps of defects. One of our research interest is to understand the details of how Apobec 3G restricts HIV-1 replication. More and more in vitro evidence suggested that Apobec 3G is a potent host restriction factor against HIV-1 replication. It is still uncertain whether Apobec 3G also influence HIV transmission and disease progression. It has been reported that HIV and AIDS disproportionately affect African Americans at a rate that is 10 times greater than is found in the U.S. White population. Since 1996, more AIDS cases have occurred among African Americans than any other U.S racial/ethnic population. A recent study reported that one codon-changing variant, H186R in exon 4 of Apobec 3G, was polymorphic in African Americans. For African Americans, the variant allele 186R was strongly associated with decline in CD4 T cells, the 186R allele was also associated with accelerated progression to AIDS-defining conditions in African Americans. It would be interesting to know whether the variation of Apobec 3G will influence HIV transmission and disease progression. We will be interested to look at the relationship between the variation of Apobec 3G and HIV transmission and disease progression among different racial group through the study of Apobec 3G genetic variation and expression levels. It will assist in determining how to improve or develop new prevention or therapeutic approaches specific to ethnic minority populations.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 载脂蛋白B mRNA编辑酶催化多肽样3G（Apobec 3G）是一种新定义的宿主抗逆转录病毒因子。属于具有胞苷脱氨酶活性的蛋白质家族。通常认为Apobec 3G通过在新合成的病毒DNA中诱导致命的G至A超突变来限制HIV-1复制。还观察到Apobec 3G在HIV-1复制期间引起病毒逆转录（RT）、DNA核输入和多步缺陷的整合。在超突变和缺陷的多个步骤之间仍然存在一些主要差距。我们的研究兴趣之一是了解Apobec 3G如何限制HIV-1复制的细节。 越来越多的体外研究表明Apobec 3G是一种有效的抗HIV-1复制的宿主限制因子。目前尚不确定Apobec 3G是否也会影响艾滋病毒的传播和疾病进展。据报道，艾滋病毒和艾滋病不成比例地影响非洲裔美国人，其发病率是美国白色人口的10倍。自1996年以来，非洲裔美国人中的艾滋病病例比美国任何其他种族/民族人口都多。最近的一项研究报告，一个密码子改变变体，H186 R在Apobec 3G外显子4，在非洲裔美国人中是多态性的。对于非裔美国人，变异等位基因186 R与CD 4 T细胞下降密切相关，186 R等位基因也与非裔美国人加速进展为艾滋病定义条件相关。了解Apobec 3G的变异是否会影响HIV传播和疾病进展将是一件有趣的事情。通过Apobec 3G基因的遗传变异和表达水平的研究，我们将有兴趣了解Apobec 3G基因变异与不同种族人群中HIV传播和疾病进展之间的关系。它将协助确定如何改进或开发针对少数民族人口的新的预防或治疗方法。