BL3 AND FLOW CYTOMETRY CORE

BL3 和流式细胞术核心

• 批准号: 8357134
• 负责人: Bindong Liu
• 金额: $ 15.72万
• 依托单位: MEHARRY MEDICAL COLLEGE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357134
• 关键词: Acquired Immunodeficiency Syndrome; Area; Cell Separation; Commit; Core Facility; Data Analyses; Doctor of Philosophy; Environment; Faculty; Flow Cytometry; Funding; Grant; HIV; HIV Infections; HIV-1; Individual; Infectious Agent; Infectious Diseases Research; Institution; Investigation; Laboratories; Maintenance; Malignant neoplasm of ovary; Methods; Molecular Biology; National Center for Research Resources; Postdoctoral Fellow; Principal Investigator; Procedures; Recruitment Activity; Research; Research Infrastructure; Research Methodology; Research Personnel; Research Support; Resources; Source; Students; Technology; Training; United States National Institutes of Health; Women' s Health; biosafety level 2 facility; biosafety level 3 facility; cost; data acquisition; experience; improved; malignant breast neoplasm; medical schools; operation; programs; protocol development

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. SPECIFIC AIMS: 1. To consolidate and improve the efficiency of operation of two core research support facilities, the BSL-3 and the FACS Core Facility. 2. To facilitate and support the research of new faculty recruited to the institution who study areas of concern in women's health. 3. To continue to provide a safe and appropriate environment for the investigation of infectious agents such as HIV by supporting the BSL-3 facility on the Meharry Medical College campus. 4. To continue to provide technological infrastructure support to research utilizing methods in flow cytometry in the investigation of molecular biology of ovarian and breast cancer, as well as HIV infection and other areas of women's health research. Bindong Liu, Ph.D., will be the Core PI and he will commit 10% effort to the conduct of this activity. Dr. Liu is the current Director of the BSL-3/FACS Core Facility and is familiar with the operation and maintenance of it. He operates a BSL-2+ facility in his laboratory and has research experience with HIV-1. He will assist faculty, post docs and students develop their research methodology to achieve their objectives as well as training the technician on the use and application of new FACS procedures and protocol development in the BSL-3. Julia Shao, MD., Senior Research Assistant (RA). She is trained in data acquisition and analysis for day-to-day operation of the FACSCalibur. She assists investigators with projects involving flow cytometry. She is able to assist users in any cell sorting required as part of the HIV and AIDS-related infectious disease research or other projects requiring the cell sorting technology. As a highly skilled individual, she is also trained in BSL-3 procedures and is able to assist and train individuals who use that facility.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 具体目标： 1. 巩固和提高两个核心研究支助设施-BSL-3和外地资产管制系统核心设施-的运作效率。 2. 促进和支持该机构新招聘的研究妇女健康问题领域的教员进行研究。 3. 通过支持梅哈里医学院校园的BSL-3设施，继续为艾滋病毒等传染病的调查提供安全和适当的环境。 4. 继续提供技术基础设施支持，以利用流式细胞术方法进行卵巢癌和乳腺癌的分子生物学调查，以及艾滋病毒感染和妇女健康研究的其他领域。 刘斌东博士，将作为核心PI，他将投入10%的精力开展这项活动。 刘博士是BSL-3/FACS核心设施的现任主任，熟悉该设施的操作和维护。他在实验室操作BSL-2+设施，并具有HIV-1的研究经验。 他将协助教师，博士后和学生开发他们的研究方法，以实现他们的目标，以及培训技术人员在BSL-3中使用和应用新的FACS程序和协议开发。 Julia Shao，医学博士，高级研究助理（RA）。她接受了FACSCalibur日常操作的数据采集和分析培训。 她协助研究人员进行涉及流式细胞术的项目。 她能够协助用户进行任何细胞分选，这些细胞分选是HIV和AIDS相关传染病研究或其他需要细胞分选技术的项目的一部分。 作为一个高度熟练的个人，她还接受了BSL-3程序的培训，能够帮助和培训使用该设施的个人。