PATHOLOGY SUPPORT FOR NTP

该主题合同的建立是为了提供广泛的常规和专业病理学服务，以支持根据国家毒理学计划（NTP）的合同进行的研究，以及通过这些研究产生的病理标本的补充研究。该合同还为NIEHS和其他NIEHS合同研究的内部调查人员进行的研究提供关键病理学（包括分子病理学）支持。具体而言，本合同支持以下服务：1)尸检和内部尸检协助；2)组织学（组织修剪和处理、载玻片制备、染色）；3)组织病理学评价/解释；4)应用专门的定性和定量形态学方法（免疫组织化学、形态计量学、细胞增殖、细胞凋亡）；5)细胞和分子生物学新技术的适应、发展、改进和应用，包括（但不限于）基因分型、DNA测序、原位杂交、DNA和RNA分离和扩增、实时、定量和原位PCR，以及这些技术应用方面的培训；6)病理同行评审（包括病理工作组）和病理评估的质量评估，并为这些活动提供技术支持；7)病理数据录入；8)病理数据或技术的展示或发表，或对这些活动的支持。在过去的一年里，本合同主要完成了以下工作：国家毒理学项目完成了9项病理和病理工作组评审；为19个内部和国家毒理学规划项目准备组织切片，其中一些项目还包括动物尸检；2个内部项目的专业病理评估；NIEHS组织和免疫组织化学实验室的现场技术支持；2个项目涉及准备病理数据以供报告或出版。此外，NTP和NIEHS还有许多其他项目正在进行中，其中一些是长期项目，涉及长期饲养动物。

The subject contract was established to provide a broad range of routine and specialized pathology services in support of studies conducted under contract by the National Toxicology Program (NTP) and for supplemental studies on pathology specimens generated through these studies. The contract also provides critical pathology (including molecular pathology) support for studies conducted by in-house investigators at NIEHS and other NIEHS contract studies. Specifically, the following services are supported by this contract: 1) necropsy and in-house necropsy assistance; 2) histology (tissue trimming and processing, slide preparation, and staining); 3) histopathological evaluation/interpretation; 4) application of specialized qualitative and quantitative morphological procedures (immunohistochemistry, morphometrics, cell proliferation, apoptosis); 5) adaptation, development, refinement, application of new techniques in cellular and molecular biology including (but not limited to) genotyping, DNA sequencing, in situ hybridization, DNA and RNA isolation and amplification, and real time, quantitative, and in situ PCR, as well as training in the application of these techniques; 6) Pathology Peer Review (including Pathology Working Groups) and Quality Assessment of pathology evaluations and technical support for these activities; 7) pathology data entry; and 8) presentation or publication of pathology data or techniques, or support for these activities. The following work was completed on this contract in the past year: 9 pathology and pathology working group reviews have been completed for the National Toxicology Program; preparation of histologic slides for 19 in-house and NTP projects some of which also included animal necropsies; specialized pathology evaluations for 2 in-house projects; on-site technical support in the NIEHS Histology and Immnohistochemistry Laboratories; and 2 projects related to preparation of pathology data for presentation or publication. In addition, there are many other projects for the NTP and NIEHS that are in progress, some of which are long term projects involving housing of animals for long periods.