National Heart, Lung, and Blood Institute Proteomics Initiative

国家心肺血液研究所蛋白质组学计划

• 批准号: 7648762
• 金额: $ 358.7万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7648762
• 关键词: National; Heart; Lung; Blood; Institute

This Proteomics Center brings together a highly interdisciplinary team to develop innovative platform technologies that analyze intracellular and secreted blood cell proteins related to systemic disease processes. This Center proposes analysis of the blood components in autoimmune disease, specifically focusing on systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). Three projects will each develop and apply novel proteomics technologies to study aberrances in the blood components in murine and human SLE and RA. In addition, novel analysis algorithms to integrate and analyze related but disparate megasets of proteomic, genomic, and clinical data will be developed. A strength of this proposal is the novel applications of existing technologies to relevant biological questions, including polychromatic flow cytometry for simultaneous measurement of multiple kinase states, microfluidic and capillary electrophoresis-based proteomic analysis of cytokines and autoantibodies, and planar protein arrays for analysis of autoantibody responses. Technology and method development tightly coupled to biological applications is a strength of this Center. The systematic acquisition of large amounts of data related to connective tissue diseases is a valuable new approach which may lead to new advances in diagnosis and treatment.

该蛋白质组学中心汇集了一个高度跨学科的团队，开发创新的平台技术，分析与全身性疾病过程相关的细胞内和分泌的血细胞蛋白。本中心建议分析自身免疫性疾病的血液成分，特别是系统性红斑狼疮（SLE）和类风湿性关节炎（RA）。三个项目将分别开发和应用新的蛋白质组学技术来研究小鼠和人类SLE和RA的血液成分异常。此外，将开发新的分析算法来整合和分析相关但不同的蛋白质组学、基因组学和临床数据。该提案的优势在于现有技术在相关生物学问题上的新应用，包括用于同时测量多种激酶状态的多色流式细胞术，基于微流体和毛细管电泳的细胞因子和自身抗体的蛋白质组学分析，以及用于分析自身抗体反应的平面蛋白质阵列。与生物应用紧密结合的技术和方法开发是本中心的优势。系统地获取与结缔组织疾病相关的大量数据是一种有价值的新方法，可能导致诊断和治疗的新进展。