National Heart, Lung, and Blood Institute Proteomics Initiative

该项目的目标是开发新的蛋白质组学技术，并应用这些方法更好地了解睡眠的分子基础。其中一组项目将涉及开发蛋白质和蛋白质检测微阵列，重点是调节蛋白质和激素。例如，将组装G蛋白偶联受体(GPCRs)阵列，其中受体-G蛋白融合被以功能形式嵌入到人造膜中。其他合成蛋白质捕捉剂阵列将促进大规模蛋白质图谱实验。预计这些工具将使我们能够开始绘制控制睡眠稳态的分子和信号转导路径。另一项主要工作将是开发监测转录因子功能的方法，重点是检测翻译后修饰蛋白并评估这些修饰的功能意义。

The objective of this program is to develop new proteomics technology and to apply these methods to better understanding the molecular basis of sleep. One set of projects will involve the development of protein and protein-detecting microarrays, with a focus on regulatory proteins and hormones. For example, arrays of G protein-coupled receptors (GPCRs) will be assembled in which receptor-G protein fusions are embedded in an artificial membrane in functional form. Other arrays of synthetic protein capture agents will facilitate large-scale protein profiling experiments. It is anticipated that these tools will allow us to begin to map the molecules and signal transduction pathways that control sleep homeostasis. Another major effort will be in the development of methods to monitor transcription factor function, with an emphasis on detecting post-translationally modified proteins and assessing the functional significance of these modifications.