National Heart, Lung, and Blood Institute Proteomics Initiative

这个项目的目标是开发新的蛋白质组学技术，并应用这些方法来更好地理解睡眠的分子基础。其中一组项目将涉及蛋白质和蛋白质检测微阵列的开发，重点是调节蛋白质和激素。例如，G蛋白偶联受体（gpcr）阵列将被组装，其中受体-G蛋白融合物以功能形式嵌入人工膜中。其他合成蛋白质捕获剂阵列将促进大规模蛋白质分析实验。预计这些工具将使我们能够开始绘制控制睡眠稳态的分子和信号转导途径。另一个主要的工作将是开发监测转录因子功能的方法，重点是检测翻译后修饰的蛋白质和评估这些修饰的功能意义。

The objective of this program is to develop new proteomics technology and to apply these methods to better understanding the molecular basis of sleep. One set of projects will involve the development of protein and protein-detecting microarrays, with a focus on regulatory proteins and hormones. For example, arrays of G protein-coupled receptors (GPCRs) will be assembled in which receptor-G protein fusions are embedded in an artificial membrane in functional form. Other arrays of synthetic protein capture agents will facilitate large-scale protein profiling experiments. It is anticipated that these tools will allow us to begin to map the molecules and signal transduction pathways that control sleep homeostasis. Another major effort will be in the development of methods to monitor transcription factor function, with an emphasis on detecting post-translationally modified proteins and assessing the functional significance of these modifications.