National Heart, Lung, and Blood Institute Proteomics Initiative

该合同的目标是开发新的质谱方法和技术，用于从小到单个细胞的样品中对整个蛋白质组进行定量分析。样品生成、技术开发和数据分析的综合方法不仅会产生新的仪器，还会产生重要的生理数据，这些数据将迅速和自由地传播。这项工作将分5个部分进行，目标如下。我。)蛋白质组学和技术发展：发展新的质谱方法和技术，用于单个细胞的整个蛋白质组的定量分析。二)。动物模型和实验系统：动物模型和实验系统组件，负责研究血管生成的动物和细胞培养模型的开发，维护和生产。III)。蛋白质分析和技术实施：通过将分析质谱（组件I）与现有分析能力相结合，实现分析质谱（组件I）的技术进步，以识别和量化血管生成过程中单个细胞或组织样本和细胞系的蛋白质组。生物信息学：将最新的信息科学技术应用于蛋白质组学。诉)管理：整合和促进科学组成部分的活动。

The goal of this contract is to develop novel mass spectrometric methodologies and technology for the quantitative analysis of the entire proteome from samples as small as individual cells. A comprehensive approach to sample generation, technology development, and data analysis will generate not only new instrumentation but also important physiological data that will be rapidly and freely disseminated. The work will be carried out in 5 separate components with the following goals and objectives. I.) Proteomics and Technology Development: Development of novel mass spectrometric methodologies and technology for the quantitative analysis of the entire proteome of a single cell. II.) Animal Models and Experimental Systems: The Animal Models and Experimental Systems Component, is charged with the development, maintenance and production of the animal and cell culture models of angiogenesis to be studied. III.) Protein analysis and Technology Implementation: To implement the technological advances in analytical mass spectrometry (component I) by merging them with existing analytical capabilities to identify and quantify the proteome of individual cells or tissue samples and cell lines during angiogenesis. IV.) Bioinformatics: To apply state-of-the-art information science techniques to applications in proteomics. V.) Administration: To integrate and facilitate the activities of the scientific components.