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National Heart, Lung, and Blood Institute Proteomics Initiative

国家心肺血液研究所蛋白质组学计划

基本信息

批准号：
7648763
负责人：
金额：
$ 474.33万
依托单位：
MEDICAL COLLEGE OF WISCONSIN
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7648763
关键词：
National Heart Lung Blood Institute

项目摘要

The goal of this contract is to develop novel mass spectrometric methodologies and technology for the quantitative analysis of the entire proteome from samples as small as individual cells. A comprehensive approach to sample generation, technology development, and data analysis will generate not only new instrumentation but also important physiological data that will be rapidly and freely disseminated. The work will be carried out in 5 separate components with the following goals and objectives. I.) Proteomics and Technology Development: Development of novel mass spectrometric methodologies and technology for the quantitative analysis of the entire proteome of a single cell. II.) Animal Models and Experimental Systems: The Animal Models and Experimental Systems Component, is charged with the development, maintenance and production of the animal and cell culture models of angiogenesis to be studied. III.) Protein analysis and Technology Implementation: To implement the technological advances in analytical mass spectrometry (component I) by merging them with existing analytical capabilities to identify and quantify the proteome of individual cells or tissue samples and cell lines during angiogenesis. IV.) Bioinformatics: To apply state-of-the-art information science techniques to applications in proteomics. V.) Administration: To integrate and facilitate the activities of the scientific components.

该合同的目标是开发新颖的质谱方法和技术，用于对小至单个细胞的样品中的整个蛋白质组进行定量分析。样本生成、技术开发和数据分析的综合方法不仅会产生新的仪器，而且会产生快速、自由传播的重要生理数据。这项工作将分 5 个独立的部分进行，其目的和目标如下。 I.) 蛋白质组学和技术开发：开发用于对单个细胞的整个蛋白质组进行定量分析的新型质谱方法和技术。 II.) 动物模型和实验系统：动物模型和实验系统部分负责开发、维护和生产要研究的血管生成的动物和细胞培养模型。 III.) 蛋白质分析和技术实施：通过将分析质谱（第一部分）的技术进步与现有的分析能力相结合，以识别和量化血管生成过程中单个细胞或组织样本和细胞系的蛋白质组，从而实现分析质谱（第一部分）的技术进步。 IV.) 生物信息学：将最先进的信息科学技术应用于蛋白质组学。 V.) 管理：整合和促进科学部分的活动。