National Heart, Lung, and Blood Institute Proteomics Initiative

国家心肺血液研究所蛋白质组学计划

• 批准号: 7648758
• 金额: $ 376.03万
• 依托单位: HENRY M. JACKSON FDN FOR THE ADV MIL/MED
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7648758
• 关键词: National; Heart; Lung; Blood; Institute

SOLICITATION NUMBER: NHLBI-HL-02-04, CONTRACT NUMBER: NO1-HV-28187 OFFEROR NAME AND ADDRESS: Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc. 1401 Rockville Pike, Suite 600 Rockville MD 20852 RFP TITLE: National Heart, Lung, and Blood Proteomics Initiative PRINCIPAL INVESTIGATOR: Dr. Harvey B. Pollard, M.D., Ph.D. 3D Proteomics in Cystic Fibrosis SUMMARY OF OBJECTIVES: The Center for Proteomics Research is a highly interactive, multidisciplinary program, designed to develop innovative proteomic technologies and apply them to the NHLBI-relevant disease of Cystic Fibrosis (CF). The goal of the present project is to deliver 3-D Proteomics, an innovative, sensitive, quantitative, and dynamic technology for enumerating the complete CF proteome from CF lung cells and CF patient biopsy tissue. The novelty in this proposal is the 3rd dimension a quantitative, biosynthetic rate for each protein in the proteome. To achieve this deliverable we propose the following: Specific Objective #1: To identify the proteins in cultured CF lung epithelial cells which are affected by CF, using the technique of 3-D proteomics. This technology involves pulsing lines of CF and isogenic control cells with 35[S] methionine or cysteine, separating proteins on 2-D gels, and detecting proteins by radioimaging. Specific Objective #2: To identify all proteins in patient samples of CF lung epithelial cells which areaffected by the disease, using 3-D Proteomics. Epithelial cells from biopsies of CF lungs will bepulse labeled with 35[S] methionine or cysteine. Significance: 3-D Proteomics, offers a rapid, sensitive and stable real time surrogate endpoint for both CF diagnosis and patient monitoring at the bedside, and a platform for discovery of novel CF therapeutics at the bench.

招标编号：NHLBI-HL-02-04，合同编号：NO1-HV-28187 供应商名称和地址： 亨利M.杰克逊基金会 军事医学促进公司 1401 Rockville Pike，Suite 600 Rockville MD 20852 国家心脏、肺和血液蛋白质组学倡议（National Heart，Lung and Blood Proteomics Initiative） 首席律师：哈维博士B。波拉德，医学博士，博士 囊性纤维化的3D蛋白质组学 证据摘要： 蛋白质组学研究中心是一个高度互动的多学科计划，旨在开发创新的蛋白质组学技术，并将其应用于囊性纤维化（CF）的NHLBI相关疾病。本项目的目标是提供3-D蛋白质组学，这是一种创新的、灵敏的、定量的和动态的技术，用于从CF肺细胞和CF患者活检组织中计数完整的CF蛋白质组。这个提议的新奇在于第三维，即蛋白质组中每种蛋白质的定量生物合成速率。为了实现这一可交付成果，我们提出了以下建议：具体目标#1：使用3-D蛋白质组学技术鉴定受CF影响的培养CF肺上皮细胞中的蛋白质。该技术涉及用35[S]甲硫氨酸或半胱氨酸脉冲CF和同基因对照细胞系，在2-D凝胶上分离蛋白质，并通过放射成像检测蛋白质。具体 目的#2：使用3-D蛋白质组学鉴定受疾病影响的CF肺上皮细胞患者样本中的所有蛋白质。将来自CF肺活检的上皮细胞用35[S]甲硫氨酸或半胱氨酸脉冲标记。重要性：三维蛋白质组学为CF诊断和床边患者监测提供了快速、灵敏和稳定的真实的时间替代终点，并为在实验室发现新型CF治疗方法提供了平台。