National Heart, Lung, and Blood Institute Proteomics Initiative

项目目标

PROJECT OBJECTIVES SOLICITATION NUMBER: NHLBI-HL-02-04 C ONTRACT NUMBER: N01-HV-28184 OFFEROR NAME AND ADDRESS: The University of Texas Medical Branch at Galveston 301 University Boulevard Galveston, Texas 77555-0657 RFP TITLE: National Heart, Lung, and Blood Institute Proteomics Initiative PRINCIPAL INVESTIGATOR: Dr. Alexander Kurosky Proteomic Technologies to Study Airway Inflammation SUMMARY OF OBJECTIVES: A comprehensive and innovative proteomics strategy will develop a model investigative approach using novel technologies generally applicable to many NHLBI research interests. Specifically, we will employ proteomics-based technologies to investigate protein expression associated with signaling pathways important in inflammatory conditions of the airways. Specific aims are to: 1) Develop sensitive high-resolution protein separation technologies to construct protein databases of changes in cellular and subcellular protein expression in the major cell types of inflamed airways; 2) Develop innovative thioaptamer-based array and bead technologies to identify and quantify proteins and protein complexes associated with critical signaling and immune response pathways relating to airway inflammation; 3) Utilize specialized high-throughput flow cytometry/cell sorting, new technology for laser zapping/imaging for cell selection, magnetic-activated cell sorting, and laser-capture microdissection for subsequent proteome analysis of defined cell types in specific stages of the cell cycle; and 4) Apply systems modeling to correlate changes in protein expression with available genomic data on changes in gene expression due to inflammation after respiratory syncytial virus infection or asthma.