Transgenic Animals

这个核心将维持现有的转基因(和其他)小鼠品系，并将提供各种不同的动物 年龄(从胚胎到老年人)到该计划内的项目。 核心的组织有两个主要的具体目标： ·保持与早老素生物学和阿尔茨海默病研究相关的转基因小鼠，并 向调查人员提供不同年龄的动物(从胚胎到老年)。 ？组织和协调各个项目的动物需求，以最大限度地提高 有效利用这一重要、昂贵和有限的资源。 本核心涵盖的动物包括表达突变(M146L)的本地小鼠或野生小鼠 人型PS1、野生型人APLP2，以及其他实验室产生的动物。 后者包括几个转基因模型，在这些模型中，A_沉积是由于 APP的突变形式(有或没有PS1的突变形式)。这些品系包括Tg2576小鼠品系 [APP-SW单独过表达]，APPswe/PS1dE9系小鼠[APPSw和APPSw都过表达 外显子9缺失型PS1]和小鼠J20系[APP-Sw，Ind]过表达。 核心还将与计划项目的调查人员合作，开发与以下相关的新品系的MICE 如果这些变得相关，他们的项目应该会变得相关。因此，核心的第三个潜在具体目标是： ？与相关调查员(S)合作，参与制定和表征 利用新的鼠标模型。 通过巩固涉及动物的努力，该计划项目寻求有效利用动物资源， 通过协调不同调查人员的请求和共享动物时 有可能。这将有助于保持动物数量和成本尽可能低，同时确保 在殖民地空间的限制内，所有项目的调查人员的需求都得到了满足。来自这里的动物 CORE也尽可能提供给方案项目之外的调查人员。

This Core will maintain existing lines of transgenic (and other) mice and will provide animals of various ages (from embryos to aged adults) to projects within this program. The Core is organized with two dominant Specific Aims: ¿ Maintaining transgenic mice relevant to the study of presenilin biology and Alzheimer disease and to provide animals of various ages (from embryos to aged adults) to investigators. ¿ Organizing and coordinating animal requirements across the individual projects to make maximal and efficient usage of this important, expensive and limited resource. Animals to be covered in this Core include locally generated mice expressing mutant (M146L) or wild type forms of human PS1, wild type human APLP2, as well as animals generated in other laboratories. The later include several transgenic models in which A_ deposition occurs as the consequence of a mutant form of APP (with or without mutant forms of PS1). These lines include the Tg2576 line of mice [over-expression of APP-Sw alone], the APPswe/PS1dE9 line of mice [with over-expression of both APPSw and the exon 9 deleted form of PS1] and the J20 line of mice [over-expression of APP-Sw,Ind ]. The Core will also work with investigators of the Program Project to develop new lines of mice relevant to their projects should these become relevant. Thus a potential third Specific Aim of the Core is to: ¿ Participate, in collaboration with the relevant investigator(s), in developing and characterizing and utilizing new mouse models. By consolidation of efforts involving animals, the Program Project seeks efficient use of animal resources, through the coordination of requests from different investigators and sharing of the animals when possible. This will help to keep animal numbers and costs are kept as low as possible while ensuring that, within the limits of colony space, the needs of investigators in all projects are met. Animals from this Core are also made available, as possible, to investigators outside of the Program Project.