EXAMINATION OF ALLOSTERIC SITE OF SEROTONIN TRANSPORTER USING TRANSGENIC MICE

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. During the reporting period, we examined the molecular and behavioral correlates of mice with mutations in this transporter, which allowed us to create a targeted transgenic knock-in mouse in which a previously characterized mutation within the serotonin transporter significantly alters the binding for specific antidepressants. Production of the transgenic construct has been completed and we have successfully created a knock-in mouse line that expresses the mutant SERT polymorphism. We continue to work to complete the screening of embryonic stem cells. Screening continues on cell lines for homologous recombinant clones of the wildtype SERT polymorphism. Our studies will continue to examine behavioral responses to acute and chronic citalopram dosing in wild-type mice.