EXAMINATION OF ALLOSTERIC SITE OF SEROTONIN TRANSPORTER USING TRANSGENIC MICE

使用转基因小鼠检查血清素转运蛋白的变构位点

• 批准号: 7715783
• 负责人: KERRY J. RESSLER
• 金额: $ 2.14万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7715783
• 关键词: Acute; Allosteric Site; Antidepressive Agents; Behavioral; Binding; Cell Line; Chronic; Citalopram; Computer Retrieval of Information on Scientific Projects Database; Dose; Funding; Genetic Polymorphism; Grant; Institution; Knock-in Mouse; Molecular; Mus; Mutation; Production; Recombinants; Reporting; Research; Research Personnel; Resources; Screening procedure; Source; Transgenic Mice; Transgenic Organisms; United States National Institutes of Health; Wild Type Mouse; Work; embryonic stem cell; mutant; response; serotonin transporter

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. During the reporting period, we examined the molecular and behavioral correlates of mice with mutations in this transporter, which allowed us to create a targeted transgenic knock-in mouse in which a previously characterized mutation within the serotonin transporter significantly alters the binding for specific antidepressants. Production of the transgenic construct has been completed and we have successfully created a knock-in mouse line that expresses the mutant SERT polymorphism. We continue to work to complete the screening of embryonic stem cells. Screening continues on cell lines for homologous recombinant clones of the wildtype SERT polymorphism. Our studies will continue to examine behavioral responses to acute and chronic citalopram dosing in wild-type mice.

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 在报告期间，我们检查了这种转运蛋白突变的小鼠的分子和行为相关性，这使我们能够创建一种靶向转基因敲入小鼠，其中5-羟色胺转运蛋白内先前表征的突变显著改变了特异性抗抑郁药的结合。 转基因构建体的生产已经完成，我们已经成功地建立了一个敲入小鼠系，表达突变SERT多态性。 我们将继续努力完成胚胎干细胞的筛选。 继续在细胞系上筛选野生型SERT多态性的同源重组克隆。 我们的研究将继续检查野生型小鼠对急性和慢性西酞普兰给药的行为反应。