EXAMINATION OF ALLOSTERIC SITE OF SEROTONIN TRANSPORTER USING TRANSGENIC MICE

使用转基因小鼠检查血清素转运蛋白的变构位点

• 批准号: 7562646
• 负责人: Kerry J Ressler
• 金额: $ 2.37万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7562646
• 关键词: Acute; Allosteric Site; Antidepressive Agents; Behavioral; Binding; Cell Line; Chronic; Citalopram; Computer Retrieval of Information on Scientific Projects Database; Dose; Funding; Grant; Institution; Knock-in Mouse; Molecular; Mus; Mutation; Production; Recombinants; Reporting; Research; Research Personnel; Resources; Screening procedure; Source; Transgenic Mice; Transgenic Organisms; United States National Institutes of Health; Wild Type Mouse; Work; design; embryonic stem cell; response; serotonin transporter; vector

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This study employs a targeted transgenic knock-in mouse in which a previously characterized mutation within the serotonin transporter significantly alters the binding for specific antidepressants. We are examining the molecular and behavioral correlates of mice with mutations in this transporter. During the reporting period, we have completed the design and production of the transgenic targeting vector and have begun working with two different transgenic facilities to transfect the constructs into embryonic stem cells. We are currently screening cell lines for homologous recombinant clones. We have also begun examining behavioral responses to acute and chronic citalopram dosing in wild-type mice.

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 这项研究采用了一种靶向转基因敲入小鼠，其中血清素转运蛋白内先前表征的突变显着改变了与特定抗抑郁药的结合。 我们正在研究这种转运蛋白突变小鼠的分子和行为相关性。 在本报告所述期间，我们完成了转基因靶向载体的设计和生产，并开始与两个不同的转基因设施合作，将构建体转染到胚胎干细胞中。 我们目前正在筛选同源重组克隆的细胞系。 我们还开始研究野生型小鼠对急性和慢性西酞普兰给药的行为反应。