UPDATE AND EXPAND VITAL RESOURCES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 亨特学院基因结构和功能研究中心(Gene Center)的科学家们在他们出色的研究项目中使用了动物。他们的调查由一个积极的机构动物护理和使用委员会(IACUC)委员会监督，并得到一个运营良好的动物设施的支持。该设施维护着各种脊椎动物物种。在过去的筹资期间，我们作出了重大努力，以升级和扩大动物护理设施。由于涉及动物的研究增加，动物普查大幅增加。为了保持对动物的充分护理和监视，现在必须聘请一名全职兽医。我们计划招募和雇用一名兽医，并用RCMI赠款资金。在下一个五年期间结束时，该机构将为这一招聘提供一条永久线路。这些 改进将使基因中心的研究科学家能够更有成效，特别是在解决健康差距的研究方面。计划中的改进，包括全职兽医，也将使该设施符合实验室动物护理评估与认可协会(AAALAC)的认证标准。 数字生物成像核心设施成立于1995年，通过数字图像处理和视频显微镜提供亚细胞结构的可视化。该设施还提供对实验数据的激光扫描分析。基因中心的研究人员利用该设施在以下方面进行开创性研究 研究领域：神经系统的损伤和修复、药物滥用的神经生物学、疼痛的分子机制、蛋白质结构和功能、行为的分子基础、免疫学和发育、纳米技术和生物有机化学。我们的计划是用一种满足我们研究需要的仪器来取代过时的十年共焦显微镜。我们还计划购买一台数字透射电子显微镜(TEM)，以取代非常旧和过时的模拟模型，以便我们可以服务于我们的研究人员的需求。这两台显微镜将安装在数字生物成像核心设施中，将利用机构资金对其进行翻新，以容纳这些仪器(见附录27)。我们也在申请RCMI拨款 支持聘请一名在制备电子显微镜样品方面具有专业知识的研究助理。这位研究助理将由该设施的经理劳埃德·威廉姆斯博士监督。拟议的改进将显著升级该设施，同时使其能够进行复杂和定量的实验，并通过互联网实时分享研究结果。这些改进将对基因中心的研究产出产生重大影响。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Scientists at the Center for Study of Gene Structure and Function (Gene Center) at Hunter College use animals in their outstanding research programs. Their investigations are monitored by an active Institutional Animal Care and Use Committee (IACUC) committee and supported by a well run Animal Facility. The facility maintains a variety of vertebrate animal species. We have made major efforts during the past funding period to upgrade and expand the Animal Care Facility. Because of the increased research involving animals, the animal census has increased significantly. To maintain adequate care and surveillance of the animals, it is now essential to hire a full-time veterinarian. We plan to recruit and hire a veterinarian with RCMI grant funds. At the end of the next five year period, the institution will provide a permanent line for this hire. These improvements will enable research scientists at the Gene Center to be more productive, especially in research that addresses health disparities. The planned improvements, including the full-time veterinarian, will also bring the facility in line with Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC) standards for certification. The Digital Bio-Imaging Core Facility, established in 1995, offers visualization of sub-cellular structures with digital image processing and video microscopy. The facility also provides for laser scanning analysis of experimental data. Gene Center Researchers use the facility to conduct pioneering research in the following areas: Damage and Repair of the Nervous System, Neurobiology of Drug Abuse, Molecular Mechanism of Pain, Protein Structure and Function, Molecular Basis of Behavior, Immunology and Development, Nanotechnology and Bio-Organic Chemistry. Our plan is to replace an obsolete ten-year-old confocal microscope with an instrument that meets our research needs. We also plan to purchase a digital transmission electron microscope (TEM) to replace a very old and obsolete analog model so that we may serve the needs of our researchers. The two microscopes will be located in the Digital Bioimaging Core Facility, which will be renovated using institutional funds to accommodate the instruments (see appendix 27. We are also requesting RCMI grant support to hire a research assistant with expertise in preparing samples for electron microscopy. This research assistant will be supervised by Dr. Lloyd Williams, the manager of the facility. The proposed improvements will significantly upgrade the facility, while making it possible to perform sophisticated and quantitative experiments and to share research results through the internet in real time. These improvements will make a major impact on the research output of the Gene Center.