UPDATE AND EXPAND VITAL RESOURCES

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 亨特学院基因结构和功能研究中心（基因中心）的科学家在其杰出的研究项目中使用了动物。他们的调查由活跃的机构动物护理和使用委员会 (IACUC) 委员会监督，并得到运行良好的动物设施的支持。该设施饲养着多种脊椎动物物种。在过去的资助期间，我们做出了重大努力来升级和扩大动物护理设施。由于涉及动物的研究增加，动物普查显着增加。为了对动物进行充分的护理和监测，现在有必要聘请一名全职兽医。我们计划利用 RCMI 赠款资金招募和雇用一名兽医。在下一个五年期结束时，该机构将为该招聘提供永久线路。这些 改进将使基因中心的研究科学家提高生产力，特别是在解决健康差异的研究方面。计划中的改进措施，包括聘请全职兽医，也将使该设施符合实验动物护理评估和认可协会 (AAALAC) 的认证标准。 数字生物成像核心设施成立于 1995 年，通过数字图像处理和视频显微镜提供亚细胞结构的可视化。该设施还提供实验数据的激光扫描分析。基因中心研究人员利用该设施在以下领域进行开创性研究 领域：神经系统的损伤和修复、药物滥用的神经生物学、疼痛的分子机制、蛋白质结构和功能、行为的分子基础、免疫学和发育、纳米技术和生物有机化学。我们的计划是用满足我们研究需求的仪器取代已经过时十年的共焦显微镜。我们还计划购买数字透射电子显微镜（TEM）来取代非常旧且过时的模拟模型，以便我们可以满足研究人员的需求。这两台显微镜将位于数字生物成像核心设施中，该设施将使用机构资金进行翻新以容纳这些仪器（见附录 27）。我们还请求 RCMI 拨款 支持聘请一名具有准备电子显微镜样品专业知识的研究助理。该研究助理将受到该设施经理劳埃德·威廉姆斯博士的监督。拟议的改进将显着升级该设施，同时使其能够进行复杂的定量实验并通过互联网实时共享研究结果。这些改进将对基因中心的研究成果产生重大影响。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Scientists at the Center for Study of Gene Structure and Function (Gene Center) at Hunter College use animals in their outstanding research programs. Their investigations are monitored by an active Institutional Animal Care and Use Committee (IACUC) committee and supported by a well run Animal Facility. The facility maintains a variety of vertebrate animal species. We have made major efforts during the past funding period to upgrade and expand the Animal Care Facility. Because of the increased research involving animals, the animal census has increased significantly. To maintain adequate care and surveillance of the animals, it is now essential to hire a full-time veterinarian. We plan to recruit and hire a veterinarian with RCMI grant funds. At the end of the next five year period, the institution will provide a permanent line for this hire. These improvements will enable research scientists at the Gene Center to be more productive, especially in research that addresses health disparities. The planned improvements, including the full-time veterinarian, will also bring the facility in line with Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC) standards for certification. The Digital Bio-Imaging Core Facility, established in 1995, offers visualization of sub-cellular structures with digital image processing and video microscopy. The facility also provides for laser scanning analysis of experimental data. Gene Center Researchers use the facility to conduct pioneering research in the following areas: Damage and Repair of the Nervous System, Neurobiology of Drug Abuse, Molecular Mechanism of Pain, Protein Structure and Function, Molecular Basis of Behavior, Immunology and Development, Nanotechnology and Bio-Organic Chemistry. Our plan is to replace an obsolete ten-year-old confocal microscope with an instrument that meets our research needs. We also plan to purchase a digital transmission electron microscope (TEM) to replace a very old and obsolete analog model so that we may serve the needs of our researchers. The two microscopes will be located in the Digital Bioimaging Core Facility, which will be renovated using institutional funds to accommodate the instruments (see appendix 27. We are also requesting RCMI grant support to hire a research assistant with expertise in preparing samples for electron microscopy. This research assistant will be supervised by Dr. Lloyd Williams, the manager of the facility. The proposed improvements will significantly upgrade the facility, while making it possible to perform sophisticated and quantitative experiments and to share research results through the internet in real time. These improvements will make a major impact on the research output of the Gene Center.