UPDATE AND EXPAND VITAL RESOURCES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 亨特学院基因结构和功能研究中心（基因中心）的科学家们在他们杰出的研究项目中使用动物。他们的研究由一个活跃的机构动物护理和使用委员会（IACUC）委员会监测，并得到一个运行良好的动物设施的支持。该设施饲养各种脊椎动物物种。在过去的资助期间，我们做出了重大努力，以升级和扩大动物护理设施。由于涉及动物的研究增加，动物普查显著增加。为了保持对动物的充分照顾和监督，现在必须雇用一名全职兽医。我们计划用RCMI的补助金招募和雇用一名兽医。在下一个五年期结束时，该机构将为这一雇用提供一个永久性的线路。这些 这些改进将使基因中心的研究科学家更有成效，特别是在解决健康差异的研究方面。计划中的改进，包括全职兽医，也将使该设施符合实验动物护理评估和认可协会（AAALAC）的认证标准。 数字生物成像核心设施，成立于1995年，提供可视化的亚细胞结构与数字图像处理和视频显微镜。该设施还提供实验数据的激光扫描分析。基因中心的研究人员利用该设施进行以下方面的开创性研究 区域：神经系统的损伤和修复，药物滥用的神经生物学，疼痛的分子机制，蛋白质结构和功能，行为的分子基础，免疫学和发育，纳米技术和生物有机化学。我们的计划是用一种能满足我们研究需要的仪器来取代一台使用了十年的过时的共聚焦显微镜。我们还计划购买一台数字透射电子显微镜（TEM），以取代一个非常古老和过时的模拟模型，以便我们可以满足我们的研究人员的需求。这两个显微镜将位于数字生物成像核心设施，该设施将利用机构资金进行翻新，以容纳这些仪器。我们还要求RCMI资助聘请一名具有电子显微镜样品制备专业知识的研究助理。该研究助理将由机构经理Lloyd威廉姆斯博士监督。拟议的改进将大大升级该设施，同时使其能够进行复杂的定量实验，并通过互联网真实的分享研究结果。这些改进将对基因中心的研究成果产生重大影响。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Scientists at the Center for Study of Gene Structure and Function (Gene Center) at Hunter College use animals in their outstanding research programs. Their investigations are monitored by an active Institutional Animal Care and Use Committee (IACUC) committee and supported by a well run Animal Facility. The facility maintains a variety of vertebrate animal species. We have made major efforts during the past funding period to upgrade and expand the Animal Care Facility. Because of the increased research involving animals, the animal census has increased significantly. To maintain adequate care and surveillance of the animals, it is now essential to hire a full-time veterinarian. We plan to recruit and hire a veterinarian with RCMI grant funds. At the end of the next five year period, the institution will provide a permanent line for this hire. These improvements will enable research scientists at the Gene Center to be more productive, especially in research that addresses health disparities. The planned improvements, including the full-time veterinarian, will also bring the facility in line with Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC) standards for certification. The Digital Bio-Imaging Core Facility, established in 1995, offers visualization of sub-cellular structures with digital image processing and video microscopy. The facility also provides for laser scanning analysis of experimental data. Gene Center Researchers use the facility to conduct pioneering research in the following areas: Damage and Repair of the Nervous System, Neurobiology of Drug Abuse, Molecular Mechanism of Pain, Protein Structure and Function, Molecular Basis of Behavior, Immunology and Development, Nanotechnology and Bio-Organic Chemistry. Our plan is to replace an obsolete ten-year-old confocal microscope with an instrument that meets our research needs. We also plan to purchase a digital transmission electron microscope (TEM) to replace a very old and obsolete analog model so that we may serve the needs of our researchers. The two microscopes will be located in the Digital Bioimaging Core Facility, which will be renovated using institutional funds to accommodate the instruments. We are also requesting RCMI grant support to hire a research assistant with expertise in preparing samples for electron microscopy. This research assistant will be supervised by Dr. Lloyd Williams, the manager of the facility. The proposed improvements will significantly upgrade the facility, while making it possible to perform sophisticated and quantitative experiments and to share research results through the internet in real time. These improvements will make a major impact on the research output of the Gene Center.