Mass spectrometry and imaging technologies in Geroscience

“质谱和成像技术在Geroscience1 P30核心将包括一个集成的 一套质谱和显微成像技术，结合特定的蛋白质和小 分子药物和代谢物分析测定和生物能量分析。P30核心的目标是 将巴克研究所独特的成像和质谱仪器设备整合起来， 针对U54的10个组成部分在结构和功能蛋白质组学中的需求， 分析药物和目标代谢物，构成这一Geroscience倡议。具体而言是 成像和质谱技术将包括：（i）功能和固定细胞成像， 蛋白质特异性荧光团和抗体，（ii）完整神经元和其它细胞的功能分析， 生物能量和氧化还原测量，（iii）蛋白质化学的开发和实施，（iv） 基于质谱的蛋白质分析，包括它们的鉴定、定量、相互作用和 翻译后修饰，（v）质谱成像和分析研究，和（vi）药物和 代谢物分析。这些研究将在一系列衰老和衰老相关疾病模型上进行 构成了U54的组成部分P30核心将由两个主要但集成的部分组成： 由Nicholls博士指导的“功能和结构成像技术”和“质谱法” 由首席研究员布拉德福德吉布森博士指导的技术。我们建议应用并整合 这些基于仪器的技术在实验设计的最早阶段， 其他研究人员开发，优化和实施这些质谱和成像 技术.

The 'Mass Spectrometry and Imaging Technologies in Geroscience1 P30 Core will consist of an integrated suite of mass spectrometry and microscopic imaging technologies coupled to specific protein and small molecule drug and metabolite analysis assays and bioenergetic analysis. The aims of this P30 Core are to integrate the unique instrumentation facilities at the Buck Institute in imaging and mass spectrometry to target the needs of the 10 components of the U54 in structural and functional proteomics, and for the analysis of drugs and targeted metabolites that constitute this Geroscience initiative. Specifically, the imaging and mass spectrometry technologies will encompass; (i) functional and fixed-cell imaging using protein-specific fluorophores and antibodies, (ii) functional analysis of intact neurons and other cells including bioenergetic and redox measurements, (iii) development and implementation of protein chemistries, (iv) mass spectrometry-based analysis of proteins, including their identification, quantitation, interactions and posttranslational modifications, (v) mass spectrometry imaging and profiling studies, and (vi) drug and metabolite analyses. These studies will be carried out on a range of aging and aging-related disease models that constitute the components of this U54. The P30 core will consist of two main but integrated parts: 'Functional and Structural Imaging Technologies' directed by Dr. Nicholls and 'Mass Spectrometry Technologies' directed by the principal investigator, Dr. Bradford Gibson. We propose to apply and integrate these instrument-based technologies at the very earliest stages of experimental design and work closely with the other investigators to develop, optimize, and implement these mass spectrometry and imaging technologies.