QSTAR Elite Quadrupole-o-TOF LC/MS/MS System

QSTAR Elite 四极杆-o-TOF LC/MS/MS 系统

• 批准号: 7389246
• 负责人: Bradford Wayne Gibson
• 金额: $ 46.56万
• 依托单位: BUCK INSTITUTE FOR RESEARCH ON AGING
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-20 至 2008-09-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7389246
• 关键词: Aging; Alzheimer' s Disease; Arts; Biological Markers; Biology of Aging; Communicable Diseases; Complex; Complex Mixtures; Computer software; Disease; Experimental Designs; Funding; Generations; Genital system; Grant; Health; Human; Huntington Disease; Institutes; Institution; Ions; Iowa; Liquid Chromatography; Lung diseases; Malignant Neoplasms; Mass Spectrum Analysis; Meningeal Tuberculosis; National Center for Research Resources; Neurodegenerative Disorders; Numbers; Otitis Media; Parkinson Disease; Peptides; Phosphorylation; Post-Translational Protein Processing; Protein Array; Proteins; Proteomics; Range; Rate; Relative (related person); Research Personnel; Stroke; System; Time; Tularemia; Ulcer; United States National Institutes of Health; Universities; age related; improved; instrument; instrumentation; ion source; malignant breast neoplasm; mass spectrometer; new technology; transmission process

DESCRIPTION (provided by applicant): This NCRR shared instrumentation proposal seeks funding for the purchase of an Applied Biosystems/Sciex new 5th generation QSTAR Elite(r) quadrupole orthogonal time-of-flight mass spectrometer and associated Tempo (Eksigent) nanocapillary multidimensional liquid chromatography (MDLC) station. To complete this integrated LC MS/MS system, we are also asking for a nanospray ion source, as well as ProteinPilot 2.0 and MarkerView software packages. In brief, we propose to operate this integrated LC MS/MS almost exclusively for on-line separation and analysis of complex peptide (and protein) mixtures as part of large group of 24 NIH funded grants from 15 investigators who have significant needs in state-of-the-art proteomics. The most critical features of this new instrumentation will be to greatly improve on the number of tandem mass spectra that can be acquired (up to 7 MS/MS spectra/sec), overall sensitivity (low attomole), dynamic range (4 logs), mass range (5-40,000 m/z), collision energetics, ion transmission, and quantitation software features. The unique capabilities of this instrumentation will be directed at a large array of protein and proteomic applications where mass spectrometry is used for protein/peptide identification in complex mixtures, relative quantitation, as well as the determination of biologically important enzymatic and non-enzymatic posttranslational modifications, including protein phosphorylation, oxidative damage and nitrosylation, among others. Overall, the acquisition of this instrument will greatly improve the throughput, experimental design, and discovery rate of 16 projects that are examining key issues of human health, including aging, neurodegenerative disease, cancer, biomarker discovery, stroke and infectious diseases. The relevance of this new instrumentation to human health is significant, as 13 independent researchers at two institutions (Buck Institute and UCSF) will use this new technology to improve their ongoing studies in the basic biology of aging and age-related diseases, including Huntington's and Alzheimer's disease, Parkinson's disease, breast cancer, and stroke. In addition, a smaller group of four investigators at the Buck Institute, UCLA and University of Iowa, that are investigating pathogenic mechanisms in several human infectious diseases including tuberculosis, meningitis, genital ulcers, respiratory disease, tularemia, and otitis media.

描述(由申请人提供)：这项NCRR共享仪器提案寻求资金，用于购买应用生物系统/Sciex新的第5代QSTAR Elite(R)四极正交飞行时间质谱仪和相关的TEMPO(Eksigent)纳米毛细管多维液相色谱(MDLC)站。为了完成这个集成的LC MS/MS系统，我们还需要一个纳米离子源，以及ProteinPilot 2.0和MarkerView软件包。简而言之，我们建议将这种集成的LC MS/MS几乎完全用于复杂多肽(和蛋白质)混合物的在线分离和分析，作为NIH资助的来自15名研究人员的24项资助的一部分，这些研究人员在最新的蛋白质组学方面有重大需求。这一新仪器最关键的特点将是大大提高可获得的串联质谱图的数量(高达7 MS/MS谱/秒)、总体灵敏度(低attomole)、动态范围(4个对数)、质量范围(5-40,000 m/z)、碰撞能学、离子传输和定量软件功能。该仪器的独特能力将针对大量的蛋白质和蛋白质组学应用，其中质谱学用于复杂混合物中的蛋白质/肽的鉴定、相对定量，以及测定具有生物重要性的酶和非酶翻译后修饰，包括蛋白质磷酸化、氧化损伤和亚硝化等。总体而言，收购该仪器将极大地提高16个项目的吞吐量、实验设计和发现率，这些项目正在研究人类健康的关键问题，包括衰老、神经退行性疾病、癌症、生物标记物发现、中风和传染病。 这一新仪器对人类健康的意义重大，因为两个机构(巴克研究所和加州大学旧金山分校)的13名独立研究人员将使用这项新技术来改进他们正在进行的关于衰老和与年龄相关的疾病的基础生物学的研究，包括亨廷顿氏症和阿尔茨海默氏症、帕金森氏病、乳腺癌和中风。此外，巴克研究所、加州大学洛杉矶分校和爱荷华大学的四名研究人员组成的一个较小的小组，正在调查几种人类传染病的致病机制，包括结核病、脑膜炎、生殖器溃疡、呼吸系统疾病、图拉热症和中耳炎。

项目成果

ScanRanker: Quality assessment of tandem mass spectra via sequence tagging.

• DOI: 10.1021/pr200118r
• 发表时间: 2011-07-01
• 期刊: JOURNAL OF PROTEOME RESEARCH
• 影响因子: 4.4
• 作者: Ma, Ze-Qiang;Chambers, Matthew C.;Ham, Amy-Joan L.;Cheek, Kristin L.;Whitwell, Corbin W.;Aerni, Hans-Rudolf;Schilling, Birgit;Miller, Aaron W.;Caprioli, Richard M.;Tabb, David L.
• 通讯作者: Tabb, David L.