AB SCIEX QTRAP 5500 LC MS/MS system

AB SCIEX QTRAP 5500 LC MS/MS 系统

• 批准号: 7794815
• 负责人: Bradford Wayne Gibson
• 金额: $ 50万
• 依托单位: BUCK INSTITUTE FOR RESEARCH ON AGING
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794815
• 关键词: Age; Aging; Alzheimer' s Disease; Biocompatible Materials; Biological Markers; Blood capillaries; Chemistry; Chronic; Data; Disease; Disease model; Electrospray Ionization; Funding; Health; Housing; Human; Human Cell Line; Huntington Disease; Infection; Institutes; Institution; Ions; Iowa; Liquid substance; Microbial Biofilms; Mus; National Center for Research Resources; Nerve Degeneration; Neurodegenerative Disorders; Organism; Parkinson Disease; Pathogenesis; Pelvic Inflammatory Disease; Peptides; Performance; Plasma; Post-Translational Protein Processing; Process; Proteins; Proteomics; Research; Research Personnel; Research Project Grants; Role; Sampling; Scanning; Screening for cancer; Source; System; Universities; age related; capillary; ear infection; human disease; human tissue; instrument; instrumentation; interest; mass spectrometer; mitochondrial dysfunction; multiple reaction monitoring; nano; nano-electrospray; novel; public health relevance

DESCRIPTION (provided by applicant): This NCRR shared instrumentation proposal seeks funding for the purchase of an integrated HPLC MS/MS system at the Buck Institute for Age Research consisting of an Applied Biosystems/Sciex QTRAP 5500 triple quadrupole linear ion-trap mass spectrometer equipped with a nano-electrospray ionization source mass spectrometer and an Eksigent/Tempo nano-capillary high performance liquid chromatograph (HPLC). This instrument will be housed and maintained at the Buck Institute's Chemistry & Proteomics Core and will serve a minimum 14 different research projects, 3 of which involve researchers at outside institution, i.e., UCSF, Gladstone Institute and the University of Iowa. In brief, we propose to operate this instrument exclusively for the targeted quantitation of peptides derived form various proteins of interest that are obtained from various biological materials relevant to human health, including mouse and human tissues, cell lines, human plasma sample, and infection organisms. Overall, these analyses will focus primarily on the requested QTRAP 5500's ability to perform multiplexed quantitation of peptides via multiple reaction monitoring (MRM). In addition, we will use this new instrument to identify and quantify a pre-select set of biologically important enzymatic and non-enzymatic posttranslational modifications using neutral loss scanning and positive/negative mode switching capabilities. The data obtained from the requested instrument will be critical to understand various disease models and paradigms. Indeed, these research projects encompass the study of neurodegenerative diseases processes such as Huntington's, Parkinson's and Alzheimer's disease, the identification of novel protein targets or biomarkers for the early detection of cancer, examining the role of mitochondrial dysfunction in aging and age-related diseases, and analysis of the role of biofilms in human bacterial pathogenesis. PUBLIC HEALTH RELEVANCE: New instrumentation is requested that will serve a variety of research projects at the Buck Institute for Age Research and neighboring institutions. The requested mass spectrometer system will allow us to carry out a variety of research projects that encompass the study of neurodegenerative (Huntington's, Parkinson's and Alzheimer's disease), the identification of novel biomarkers for the early detection of cancer, determining the role of mitochondrial dysfunction in aging and age-related diseases, and the analysis of the bacterial biofilms that form in some human diseases, such as chronic ear infections and pelvic inflammatory disease.

描述（由申请人提供）：本NCRR共享仪器提案寻求资助，用于在Buck年龄研究所购买集成HPLC MS/MS系统，该系统由配备纳米电喷雾电离源质谱仪的Applied Biosystems/Sciex QTRAP 5500三重四极杆线性离子阱质谱仪和Eksigent/克里思纳米毛细管高效液相色谱仪（HPLC）组成。该仪器将被安置和维护在巴克研究所的化学和蛋白质组学核心，并将服务于至少14个不同的研究项目，其中3个涉及外部机构的研究人员，即，加州大学旧金山分校、格莱斯顿研究所和爱荷华州大学。简而言之，我们建议操作该仪器专门用于靶向定量从各种感兴趣的蛋白质衍生的肽，这些蛋白质从与人类健康相关的各种生物材料中获得，包括小鼠和人类组织、细胞系、人血浆样品和感染生物体。总体而言，这些分析将主要关注所要求的QTRAP 5500通过多反应监测（MRM）对肽进行多重定量的能力。此外，我们将使用这种新的仪器，以确定和量化一个预先选择的生物重要的酶和非酶的翻译后修饰，使用中性丢失扫描和正/负模式切换功能。从所要求的仪器获得的数据对于理解各种疾病模型和范例至关重要。事实上，这些研究项目包括神经退行性疾病过程的研究，如亨廷顿氏病，帕金森氏病和阿尔茨海默氏病，识别新的蛋白质靶点或生物标志物用于早期检测癌症，检查线粒体功能障碍在衰老和年龄相关疾病中的作用，以及分析生物膜在人类细菌发病机制中的作用。 公共卫生相关性：新的仪器要求，将服务于各种研究项目在巴克研究所年龄研究和邻近机构。所要求的质谱仪系统将使我们能够开展各种研究项目，包括神经退行性疾病的研究。（亨廷顿氏病、帕金森氏病和阿尔茨海默氏病）、鉴定用于癌症早期检测的新型生物标志物、确定线粒体功能障碍在衰老和年龄相关疾病中的作用以及分析在一些人类疾病中形成的细菌生物膜，例如慢性耳部感染和盆腔炎。