TripleTOF 5600 High Resolution Mass Spectrometer

描述（由申请人提供）： 该共享仪器提案寻求购买AB Sciex下一代TripleTOF 5600四极正交飞行时间质谱仪的资金。该质谱仪将是集成的纳米-2DLC MS/MS系统的一部分，该系统将通过巴克研究所为此目的已经承诺的独立资金完成。简而言之，我们建议操作这个集成的TripleTOF质谱系统，专门用于在线分离和分析复杂的肽和蛋白质混合物，作为来自Buck研究所的12名独立主要研究人员和6名外部研究人员组成的大组的一部分，这些研究人员代表了总共7个机构或大学。这些研究汇集了21项NIH资助的赠款，所有这些研究都需要最先进的质谱技术，用于各种蛋白质组学应用，这些应用需要最高的采样效率，分辨率和灵敏度。这种新仪器的最重要的特点是MS 1（40，000）和MS 2（15，000 - 35，000）的高分辨率数据采集能力，在给定时间内可以采集的串联质谱的数量（通常为35次扫描/秒），灵敏度（低阿托摩尔）和高动态范围。这些操作参数还将允许几种独特的数据采集扫描，包括SWATH和伪MRM，以及使用MS 1和MS 2离子强度数据的新型定量方法。该仪器的独特功能将针对各种蛋白质和蛋白质组学应用，其中质谱法用于非常复杂的生物样品的鉴定，发现和全球定量。生物学上重要的酶和非酶翻译后修饰的鉴定和定量将是这种新质谱仪的关键用途，包括蛋白质磷酸化、赖氨酸乙酰化和氧化损伤等。总的来说，在巴克老龄化研究所的化学与质谱核心中收购该仪器将大大提高超过19个项目的吞吐量，实验设计和发现率，这些项目正在研究人类健康和健康范围的关键问题，包括衰老生物学，神经退行性疾病，糖尿病，癌症，疾病生物标志物发现和传染病。

DESCRIPTION (provided by applicant): This shared instrumentation proposal seeks funding for the purchase of an AB Sciex next generation TripleTOF 5600 quadrupole orthogonal time-of-flight mass spectrometer. This mass spectrometer will be part of an integrated nano-2DLC MS/MS system to be completed through independent funds already committed by the Buck Institute for this purpose. In brief, we propose to operate this integrated TripleTOF mass spectrometry system exclusively for the on-line separation and analysis of complex peptide and protein mixtures as part of large group of 12 independent principal investigators from the Buck Institute and 6 external investigators that represent a total of seven institutions or universities. Together, these researches bring together 21 NIH funded grants, all of which have significant needs for state-of-the-art mass spectrometry for various proteomics applications that require the highest sampling efficiency, resolution and sensitivity. The most critical features of this new instrumentation will be to the high resolution capabilities of data acquisition in both MS1 (40,000) and MS2 (15,000 - 35,000), the number of tandem mass spectra that can be acquired in a given time (typically 35 scans /sec), the exquisite sensitivity (low attomoles) and high dynamic range. These operating parameters will also allow several unique data acquisition scans, including SWATH and pseudo-MRM, and novel quantitation approaches that uses both MS1 and MS2 ion intensity data. The unique capabilities of this instrumentation will be directed at a diverse array of protein and proteomic application where mass spectrometry is used for identification, discovery, and global quantitation in very complex biological samples. The identification and quantitation of biologically important enzymatic and non-enzymatic posttranslational modifications will be a critical use of this new mass spectrometer, including protein phosphorylation, lysine-acetylation, and oxidative damage, among others. Overall, the acquisition of this instrument to the Chemistry & Mass Spectrometry Core at the Buck Institute for Research on Aging will greatly improve the throughput, experimental design, and discovery rate of over 19 projects that are examining key issues of human health and health span, including the biology of aging, neurodegenerative disease, diabetes, cancer, disease biomarker discovery, and infectious diseases.