IMMUNOHISTOCHEMICAL IDENTIFICATION OF NONHUMAN PRIMATE ANTIGENS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 为了提高我们的诊断病理学能力，我们系统地评估了针对人细胞/组织和感染因子的市售抗体，以应用于NEPRC饲养的非人灵长类动物。 选择抗体用于诊断和/或实验病理学中的潜在应用，以及用于速冻或福尔马林固定的石蜡包埋组织中的反应性。 此外，比较病理学部门每年都会收到多个请求，要求为我们部门的研究人员，其他NEPRC部门和合作研究人员测试抗体并制定IHC方案。 因此，在非人灵长类动物中鉴定免疫组织化学抗体的研究和开发是分子病理学核心的一项重要活动，并有助于开发在猕猴组织中一致发挥作用的多种IHC抗体库。 在一项研究中，我们能够识别4种猕猴中21种抗原中的21种和新世界猴中21种抗原中的17种，并且在2005年期间，我们开发了21种在猴组织中交叉反应的新抗体的免疫组织化学方案。 比较病理学系还培训研究人员、技术人员、博士后研究员和研究生免疫组织化学方法。 艾滋病相关

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In order to improve our diagnostic pathology capabilities, we systematically evaluate commercially available antibodies directed against human cells/tissues and infectious agents for application in the nonhuman primate species housed at the NEPRC. Antibodies are selected for potential applications in diagnostic and/or experimental pathology, and for reactivity in either snap-frozen or formalin-fixed, paraffin-embedded tissues. In addition, the Division of Comparative Pathology receives multiple requests each year to test antibodies and develop IHC protocols for investigators in our Division, other NEPRC Divisions, and collaborating investigators. Thus, research and development to identify antibodies for immunohistochemistry in nonhuman primates is an important activity of the Molecular Pathology Core, and has contributed to the development of a diverse repertoire of IHC antibodies that work consistently in macaque tissue. In one study we were able to identify 21 of 21 antigens in 4 macaque species and 17 of 21 in New World monkeys, and during 2005, we developed immunohistochemistry protocols for 21 new antibodies that were cross-reactive in monkey tissues. The Division of Comparative Pathology also trains investigators, technicians, postdoctoral fellows and graduate students in immunohistochemistry methods. AIDS related.