DIET INDUCED OBESITY IN PAPIO: PALATABLE HIGH-FAT HIGH-FRUCTOSE DIET

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 本研究的目的是建立一种非人灵长类动物的饮食诱导肥胖模型。我们打算评估瘦狒狒体内脂肪积累加速发展的程度，这些狒狒长期维持高可口的致肥饮食（高胆固醇、高脂肪、高简单碳水化合物）。现在已经清楚地表明，肥胖是冠心病（CHD）和2型糖尿病（T2 D）发展的主要（和可预防的）风险因素，主要通过其对脂质和葡萄糖代谢的病理影响。我们自己在SFBR过去十年的工作清楚地表明，一些狒狒即使在保持标准食物饮食（低脂肪;低简单碳水化合物）的情况下也容易自发性肥胖。 鉴于狒狒对体重增加的明显易感性，我们假设我们可以通过饮食控制加速肥胖及其相关合并症（CHD和T2 D）的发展。具体来说，通过增加饮食中的脂肪和简单碳水化合物含量，使其更能代表西方社会中大多数人日益消费的饮食，我们可以预见地诱导狒狒肥胖。本研究将使用约20只动物，这些动物将接受适口的高脂肪-高热量密集饲料（自由采食）激发。他们中的一半人还将在六个月内每天获得富含糖的饮料（使用高果糖玉米糖浆）。最后10只动物（共30只）将作为对照组，保持正常饮水和自由采食标准饲料。挑战将在六个月内进行。 将记录人体测量结果，并在基线时和研究期间每两个月采集一次血样。身体脂肪成分也将每两个月测量一次。总之，我们建议用“致肥胖-高度适口-致动脉粥样硬化”饮食喂养20只动物，该饮食将模仿典型快餐餐的饮食组成（例如，一个汉堡包，大薯条和大苏打水），但可以在正常的住房条件下喂养我们的殖民地。制备适口颗粒的成分是：猪油、高果糖玉米糖浆、Crisco、Hydrog。椰子油、胆固醇粉、盐、维生素混合物和蛋黄，这些颗粒将使用饮食厨房现场生产。将对颗粒进行烘焙，以增加食物来源的适口性。因此，当前初步研究的具体目的是制定一种非常可口的致肥胖饮食，并使一组成年狒狒保持这种饮食6个月，以评价其对体重以及脂质和葡萄糖状态的影响。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The aim of this pilot project is to establish a non-human primate model of diet induced obesity (DIO). We intend to assess the extent of accelerated development of body fat accumulation in lean baboons maintained for an extended period on a highly palatable obesogenic diet (high cholesterol; high fat; high simple carbohydrates). It has now been clearly demonstrated that obesity represents a major (and preventable) risk factor for the development of Coronary Heart Disease (CHD) and Type 2 Diabetes (T2D), primarily through its pathological effects on lipid and glucose metabolism. Our own work over the past decade here at SFBR has clearly shown that some baboons are susceptible to the development of spontaneous obesity even while being maintained on a standard chow diet (low fat; low simple carbohydrates). Given this apparent susceptibility of baboons to weight gain, we hypothesize that we can accelerate the development of obesity and its associated comorbidities (CHD and T2D) by dietary manipulation. Specifically by increasing both the fat and simple carbohydrate content of the diet to make it more representative of the diets increasingly consumed by most individuals in westernized societies we can predictably induce obesity in baboons. This study will use approximately 20 animals that will be challenged with a palatable high fat-high calorie dense diet (fed ad libitum). Half of them will also be given access to a sugar-enriched drink (using high fructose corn syrup) every day for six months. The final 10 animals (30 total) will serve as controls having been maintained on regular water and the standard chow diet offered ad libitum. The challenge will be conducted over a six month period. Anthropometric measurements will be recorded and blood samples will be drawn at baseline and every two months through the course of the study. Body fat composition will also be measured every two months. In summary, we are proposing to feed 20 animals with an "obesogenic-highly palatable-atherogenic" diet which will mimic the dietary composition of a typical fast food meal (e.g., a hamburger, large fries and large soda) but which can be fed under the normal housing conditions for our colony. The ingredients to prepare the palatable pellets are: Lard, High Fructose Corn Syrup, Crisco, Hydrog. coconut oil, Cholesterol in powder, Salt, Vitamin mixture, and Egg yolk and these pellets will be produced on site using the diet kitchen. The pellets will be baked to add palatability to the food source. Therefore the specific aim of the current pilot study will be to formulate a highly palatable obesogenic diet and maintain a group of adult baboons on this diet for six months to evaluate its effects on body weight as well as lipid and glucose status.