CORRELATION OF MATRIX METALLOPROTEINASE AND FUNCTIONAL OUTCOME FOLLOWING TRAUMA

基质金属蛋白酶与创伤后功能结果的相关性

• 批准号: 7716620
• 负责人: GARY ROSENBERG
• 金额: $ 1.48万
• 依托单位: UNIVERSITY OF NEW MEXICO
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2008-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7716620
• 关键词: Admission activity; Biochemical; Brain; Brain Injuries; Catheters; Cerebral Ventricles; Cerebrospinal Fluid; Cerebrospinal Fluid Pressure; Cerebrospinal Fluid Proteins; Computer Retrieval of Information on Scientific Projects Database; Excision; Funding; Gelatinase A; Grant; Hour; Injury; Institution; Intercellular Fluid; Matrix Metalloproteinases; Measurement; Measures; Methods; Monitor; Outcome; Patients; Plasma; Research; Research Personnel; Resources; Sampling; Score; Source; Time; Trauma; Traumatic Brain Injury; United States National Institutes of Health; Ventriculostomy; cognitive function; cohort; functional outcomes; injured; nervous system disorder; pressure

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Traumatic brain injury (TBI) increases Matrix Metalloproteinase (MMP)-2 and -9 and the increase in these levels correlate with the Glasgow Outcome Score (GOS) and Rancho Los Amigos Cognitive Functioning Scale (LCFS). Specific aims: Patients with severe brain injury undergo monitoring of the cerebrospinal fluid (CSF) pressure by the insertion of a catheter into the cerebral ventricles. The pressure is monitored and the CSF is withdrawn when the pressure is elevated. CSF reflects the changes in the brain interstitial fluid and measurements of substrates in the CSF provide an indication of the biochemical changes that are occurring in the brain. An increase in CSF proteins, including MMPs, occurs in several neurological diseases. We plan to measure the levels of MMPs in the CSF by a sensitive zymographic method. Aim 1: To carefully select an appropriate TBI cohort for MMP level analysis in plasma and CSF and to obtain samples of CSF from brain injured patients at the time of admission, 24 and 72 hours after the injury, and at the time of the removal of the ventriculostomy. Aim 2: To establish the time course of MMP-2 and -9 expression and activation in CSF and plasma. Aim 3: To determine the correlation of GOS and LCFS to MMP levels.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 创伤性脑损伤（TBI）增加基质金属蛋白酶（MMP）-2和-9，并且这些水平的增加与格拉斯哥结局评分（GOS）和Rancho Los Amigos认知功能量表（LCFS）相关。 具体目标：患有严重脑损伤的患者通过将导管插入脑室来监测脑脊液（CSF）压力。 监测压力，并在压力升高时抽取CSF。 CSF反映了脑间质液的变化，CSF中底物的测量提供了脑中发生的生化变化的指示。 CSF蛋白（包括MMP）的增加发生在几种神经系统疾病中。 我们计划通过灵敏的酶谱法测量CSF中MMPs的水平。 目标1：仔细选择适当的TBI队列进行血浆和CSF中MMP水平分析，并在入院时、损伤后24和72小时以及移除脑室造口术时从脑损伤患者中获取CSF样本。 目的2：建立MMP-2和MMP-9在脑脊液和血浆中表达和活化的时间过程。 目的3：探讨MMP与GOS、LCFS的相关性。