Specimen Acquisition and Pathology

标本采集和病理学核心是SPORE的关键组成部分，将支持 SPORE项目的转化目标，以及通过SPORE资助的发展项目。博士 波特的领导不仅将提供研究和临床乳腺病理学专业知识， 核心B活动与整个SPORE，特别是开发项目。这种协调 标本的采集和分发以及新研究的推广将大大有助于基础研究的翻译。 将新发现转化为涉及人体样本的实验核心增强了现有的强大的FHCRC/UW 癌症联盟乳腺标本库和登记处（BSRR），并将集中 SPORE所需样本的采集、处理、病理分析和分发 项目它还将为选择生物标志物测定提供实验室基础，以支持主要的 发展项目。 具体而言，核心将： 1)使用已建立的有效的BSRR采购和分发组织和血液样本， SPORE和发展项目 2)为SPORE和开发项目执行方案特定的标本处理 3)维护一个数据库，允许样本跟踪和分发，与相关的去识别 数据，以及与其他项目和核心的数据共享 4)为SPORE和开发项目提供高质量的病理学支持，包括： <$病理学审查的组织在不同层次的复杂性 组织学切片和切片切片的制备，用于组织学评价 免疫组织化学（IHC）和新抗体的检查和优化 免疫过氧化物酶和免疫荧光染色的IHC解释 激光捕获显微切割（LCM） 组织微阵列（TMA）设计、构建、切片和成像

The Specimen Acquisition and Pathology Core is a key component of the SPORE and will support the translational goals of SPORE Projects, as well as developmental projects funded through the SPORE. Dr. Porter's leadership will provide not only research and clinical breast pathology expertise but also integration of Core B activities with the SPORE overall, and developmental projects in particular. This coordination of specimen acquisition and distribution with promotion of new research will greatly aid translation of basic discoveries into experiments involving human samples. The Core augments the existing robust FHCRC/UW Cancer Consortium Breast Specimen Repository and Registry (BSRR) and will function to centralize specimen acquisition, processing, pathologic analysis and distribution of samples needed by SPORE projects. It will also provide a laboratory base for select biomarker assays in support of the major and developmental projects. Specifically the Core will: 1) Use the established and efficient BSRR to procure and distribute tissue and blood specimens for SPORE and developmental projects 2) Perform protocol-specific specimen processing for SPORE and developmental projects 3) Maintain a database that allows specimen tracking and distribution, linkage with relevant deidentified data, and data sharing with other projects and cores 4) Provide high-quality pathology support for SPORE and developmental projects including: ¿ Pathology review of tissues at various levels of complexity ¿ Histology sectioning and preparation of section slides for histologic evaluation ¿ Immunohistochemistry (IHC), and new antibody work-up and optimization ¿ IHC interpretation of both immunoperoxidase and immunofluorescence stains ¿ Laser capture microdissection (LCM) ¿ Tissue microarray (TMA) design, construction, sectioning, and imaging