Alcohol-Induced Bone Resorption: The Role of Oxidative Stress

酒精引起的骨吸收:氧化应激的作用

基本信息

  • 批准号:
    7890356
  • 负责人:
  • 金额:
    $ 34.56万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2009
  • 资助国家:
    美国
  • 起止时间:
    2009-07-10 至 2014-06-30
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): Although it is well known that women are more susceptible to the toxic effects of ethanol (EtOH) than men, much less is known about the molecular mechanisms underlying alcohol toxicity in women especially as relates to bone. Alcohol abuse during early adulthood results in impaired bone growth and in the U.S.A. approximately 20% of women age 18-30 (4.4 million) binge drink. A resulting reduction in peak bone mass would predispose women to osteoporosis in later life. The molecular mechanisms underlying the toxic effects of EtOH on bone remain poorly understood. EtOH, particularly at higher concentrations characteristic of binge drinking, can increase bone resorption. We have developed a new mouse model to study alcohol-induced bone loss in cycling females. Chronic consumption of EtOH as part of liquid diets using this system produces EtOH-induced bone loss without compromising food intake. Bone loss in female mice was accompanied by increased serum markers of bone resorption and increased osteoclast numbers in ex-vivo bone marrow cultures. These data are consistent with previously published data from our laboratory in cycling female rats fed ethanol via total enteral nutrition. In the rat model, increased bone resorption was accompanied by increased expression of RANKL in bone. RANKL is a member of the TNF family expressed on the surface of osteoblasts which signals through the receptor RANK on the surface of osteoclast precursors to stimulate osteoclastogenesis. EtOH-induction of RANKL occurred in primary osteoblast cultures from rats and mice and in rat and mouse UMR-106 and ST-2 osteoblast-like cell lines. EtOH-induction of RANKL was blocked by the antioxidant N-acetylcysteine (NAC) in vitro. RANKL appears to be regulated via ERK1/2 and STAT3 phosphorylation. In vitro data suggest that the ERK/STAT3/RANKL pathway is stimulated by reactive oxygen species (ROS) produced by an ethanol-induced NADPH oxidase (NOX4). Expression of this enzyme in osteoblasts is increased by EtOH and blocked by NAC. The mouse liquid diet model will be utilized to determine the role of NOX4 and oxidative stress in EtOH-induced bone resorption in vivo using p47phox -/- mice and catalase transgenic mice. In vitro, the mouse ST-2 cell line, differentiated mouse stromal osteoblast cultures and osteoblast/osteoclast precursor co-cultures will be utilized to probe the molecular cascade from EtOH to RANKL and the inhibition of EtOH-induced RANKL by NAC and other antioxidants. NOX gain and loss of function studies will be performed by transient transfection and use of siRNA and the effects of hydrogen peroxide and free radical scavenging on the ERK/STAT3/RANKL cascade will be examined. In addition, in vivo studies will be conducted to examine the ability of antioxidants to block EtOH-induced bone resorption. PUBLIC HEALTH RELEVANCE: Excessive alcohol consumption is an increasing problem in young women. The results from research proposed herein will link the deleterious effects of alcohol consumption on bone in women of reproductive age with osteoporosis, a major cause of morbidity and mortality in postmenopausal women. Alcohol abuse results in osteopenia and increases osteoporosis risk. One potentially important yet under-explored area of bone health is the relationship between bone resorption associated with alcohol abuse in young women and increased risk of osteoporosis which results from impairments in attainment of peak bone mass. In addition, alcohol-induced bone loss shares many features in common with bone loss during menopause and aging. Our animal data suggests that dietary antioxidants may prevent alcohol-induced bone resorption and we will test this in the current application. Molecular studies of alcohol actions on ROS signaling cascades in bone are expected to provide fundamental insights into common pathways underlying bone resorption.
描述(由申请人提供):尽管众所周知女性比男性更容易受到乙醇(EtOH)毒性作用的影响,但对女性酒精毒性的分子机制知之甚少,尤其是与骨相关的机制。成年早期的酒精滥用会导致骨骼生长受损,在美国,大约20%的18-30岁的女性(440万人)酗酒。由此导致的峰值骨量减少将使妇女在以后的生活中容易患骨质疏松症。EtOH对骨毒性作用的分子机制仍然知之甚少。乙醇,特别是在高浓度下的酗酒特征,可以增加骨吸收。我们开发了一种新的小鼠模型来研究骑自行车的女性中酒精引起的骨丢失。使用该系统长期消耗EtOH作为液体饮食的一部分,产生EtOH诱导的骨丢失,而不影响食物摄入。雌性小鼠的骨丢失伴随着骨吸收的血清标志物增加和体外骨髓培养中破骨细胞数量增加。这些数据与我们实验室先前发表的通过全肠内营养饲喂乙醇的骑自行车雌性大鼠的数据一致。在大鼠模型中,骨吸收增加伴随着骨中RANKL表达增加。RANKL是在成骨细胞表面上表达的TNF家族的成员,其通过破骨细胞前体表面上的受体RANK发出信号以刺激破骨细胞生成。在大鼠和小鼠的原代成骨细胞培养物中以及在大鼠和小鼠UMR-106和ST-2成骨细胞样细胞系中发生RANKL的EtOH诱导。在体外,抗氧化剂N-乙酰半胱氨酸(NAC)可阻断EtOH诱导的RANKL。RANKL似乎通过ERK 1/2和STAT 3磷酸化调节。体外数据表明,ERK/STAT 3/RANKL通路受到乙醇诱导的NADPH氧化酶(NOX 4)产生的活性氧(ROS)的刺激。这种酶在成骨细胞中的表达被EtOH增加并被NAC阻断。使用p47 phox-/-小鼠和过氧化氢酶转基因小鼠,采用小鼠流质饮食模型确定NOX 4和氧化应激在体内EtOH诱导的骨吸收中的作用。在体外,小鼠ST-2细胞系、分化的小鼠基质成骨细胞培养物和成骨细胞/破骨细胞前体共培养物将用于探测从EtOH到RANKL的分子级联反应以及NAC和其他抗氧化剂对EtOH诱导的RANKL的抑制。将通过瞬时转染和使用siRNA进行NOX获得和功能丧失研究,并检查过氧化氢和自由基清除对ERK/STAT 3/RANKL级联的影响。此外,将进行体内研究,以检查抗氧化剂阻断EtOH诱导的骨吸收的能力。公共卫生相关性:过度饮酒是年轻女性日益严重的问题。本文提出的研究结果将把饮酒对育龄妇女骨骼的有害影响与骨质疏松症联系起来,骨质疏松症是绝经后妇女发病和死亡的主要原因。酗酒导致骨质减少,增加骨质疏松症的风险。骨健康的一个潜在的重要但未充分探索的领域是年轻女性中与酒精滥用相关的骨吸收与骨质疏松症风险增加之间的关系,骨质疏松症风险增加是由于达到峰值骨量的障碍。此外,酒精引起的骨质流失与绝经和衰老期间的骨质流失有许多共同特征。我们的动物数据表明,膳食抗氧化剂可以防止酒精诱导的骨吸收,我们将在当前的应用中测试这一点。酒精作用于骨中ROS信号级联的分子研究有望为骨吸收的共同途径提供基本的见解。

项目成果

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Martin J J Ronis其他文献

Martin J J Ronis的其他文献

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{{ truncateString('Martin J J Ronis', 18)}}的其他基金

The role of oxidative stress in alcohol-induced osteopenia
氧化应激在酒精引起的骨质减少中的作用
  • 批准号:
    10406154
  • 财政年份:
    2021
  • 资助金额:
    $ 34.56万
  • 项目类别:
The role of oxidative stress in alcohol-induced osteopenia
氧化应激在酒精引起的骨质减少中的作用
  • 批准号:
    10608146
  • 财政年份:
    2021
  • 资助金额:
    $ 34.56万
  • 项目类别:
The role of oxidative stress in alcohol-induced osteopenia
氧化应激在酒精引起的骨质减少中的作用
  • 批准号:
    9344518
  • 财政年份:
    2016
  • 资助金额:
    $ 34.56万
  • 项目类别:
The role of oxidative stress in alcohol-induced osteopenia
氧化应激在酒精引起的骨质减少中的作用
  • 批准号:
    9919470
  • 财政年份:
    2016
  • 资助金额:
    $ 34.56万
  • 项目类别:
The role of oxidative stress in alcohol-induced osteopenia
氧化应激在酒精引起的骨质减少中的作用
  • 批准号:
    9126707
  • 财政年份:
    2016
  • 资助金额:
    $ 34.56万
  • 项目类别:
Prevention of EtOH-induced promotion of hepatocarcinogenesis by genistein/soy
金雀异黄素/大豆预防乙醇诱导的肝癌发生
  • 批准号:
    8354189
  • 财政年份:
    2012
  • 资助金额:
    $ 34.56万
  • 项目类别:
Prevention of EtOH-induced promotion of hepatocarcinogenesis by genistein/soy
金雀异黄素/大豆预防乙醇诱导的肝癌发生
  • 批准号:
    8547039
  • 财政年份:
    2012
  • 资助金额:
    $ 34.56万
  • 项目类别:
Alcohol-Induced Bone Resorption: The Role of Oxidative Stress
酒精引起的骨吸收:氧化应激的作用
  • 批准号:
    8497553
  • 财政年份:
    2009
  • 资助金额:
    $ 34.56万
  • 项目类别:
Alcohol-Induced Bone Resorption: The Role of Oxidative Stress
酒精引起的骨吸收:氧化应激的作用
  • 批准号:
    8299084
  • 财政年份:
    2009
  • 资助金额:
    $ 34.56万
  • 项目类别:
Alcohol-Induced Bone Resorption: The Role of Oxidative Stress
酒精引起的骨吸收:氧化应激的作用
  • 批准号:
    8100109
  • 财政年份:
    2009
  • 资助金额:
    $ 34.56万
  • 项目类别:

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