STRUCTURE AND FUNCTION OF THE OUTER HAIR CELL MOTOR PROTEIN, PRESTIN

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 Fisher正在利用光亲和标记、机械测量以及与我们的实验室合作的质谱学来阐明外毛细胞发动机蛋白prestin的结构和功能。由于他正在研究的叠氮修饰配体似乎以惊人的效率与prestin结合，他很可能能够通过使用我的实验室开发的质谱学和数据分析技术来识别共价修饰的残基。这将使他能够研究Prestin与小羧酸的假定结合部位。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Fisher is utilizing photoaffinity labeling, mechanical measurements, and, in collaboration with our lab, mass spectrometry to elucidate the structure and function of the outer hair cell motor protein, prestin. Because the azide-modified ligand that he is working with appears to bind to prestin with remarkable efficiency, it is likely that he will be able to identify covalently modified residues by using mass spectrometric and data analysis techniques that my lab has developed. This will permit him to investigate prestin's putative binding site for small carboxylic acids.