Interspecies Streptococcal Antagonisms in Oral Biofilms

口腔生物膜中的种间链球菌拮抗作用

基本信息

批准号：
8015639
负责人：
Jens Kreth
金额：
$ 23.69万
依托单位：
UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-02-16 至 2013-01-31
项目状态：
已结题

项目摘要

ABSTRACT The role of S. mutans in the etiology of dental caries has been well investigated. S. mutans resides in the dental plaque with up to 70 other bacterial species. Recent advances in research suggest that the cariogenic potential of dental plaque may be detemiined by the combined interactions ofthe entire oral bacteria. These interactions include modulation of gene expression, co-aggregation and growth inhibition. We hypothesize that oral streptococci compete In part by influencing virulence gene expression in the antagonistic species'by direct physical Interactions and/or metabolic signaling. The proposed Aims are designed to characterize the virulence genes of S. mutans and S. sanguinis and to identify the mechanisms how oral bacteria influence the expression of virulence gene(s) of other species. We chose the interaction of S. mutans and S. sanguinis as ideal model system because of their long known mutual exclusion. To test our hypothesis that interspecies interactions influence the outcome of the competition between S. mutans and S. sanguinis, we will: ¿ Develop a dual-species biofilm model based on saliva coated hydroxyapatite for reproducible investigation of S. mutans and S. sanguinis biofilm formation ¿ Search for new genes involved in the interspecies interaction between S. mutans and S. sanguinis ¿ Determine the expression profile of S. mutans and S. sanguinis virulence genes in response to each other ¿ Further characterize the direct physical interactions between S. mutans and S. sanguinis by atomic force microscopy

抽象的链球菌在龋齿病因中的作用已经得到充分研究。 s 在牙菌斑中，有多达70种细菌的牙齿中的叛变住宅。最近的研究的进展表明，牙菌斑的致癌潜力可能是由整个口腔细菌的组合相互作用所掩盖。这些相互作用包括调节基因表达，共聚集和生长抑制。我们假设口服链球菌部分通过影响病毒基因竞争通过直接的物理相互作用和/或代谢中的拮抗物种中的表达信号。所提出的目的旨在表征S的病毒基因。 Mutans和S. sanguinis，并确定口服细菌如何影响的机制其他物种的病毒基因的表达。我们选择S. mutans的相互作用 S. sanguinis是理想的模型系统，因为它们已知的相互排斥。为了检验我们的假设，即种间相互作用会影响 S. Mutans和S. sanguinis之间的竞争，我们将： �开发基于唾液涂层羟基磷灰石的双物种生物膜模型 S. mutans和S. sanguinis生物膜形成的可再现投资 »搜索与S. mutans之间的种间相互作用有关的新基因和S. sanguinis �确定葡萄链球链球菌的表达曲线和S. sanguinis病毒基因彼此回应 »进一步表征了链球菌与S.之间的直接物理相互作用。原子力显微镜