Role of Streptococcus mutans eukaryotic-type serine/threonine protein kinase in interspecies interactions with Streptococcus sanguinis.
Role of Streptococcus mutans eukaryotic-type serine/threonine protein kinase in interspecies interactions with Streptococcus sanguinis.
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DOI：
10.1016/j.archoralbio.2010.03.012
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发表时间：
2010-05
影响因子：
3
通讯作者：
Kreth, Jens
中科院分区：
文献类型：
--
作者：
Zhu, Lin;Kreth, Jens
口腔链球菌的种间相互作用涉及抗菌化合物的产生和排泄，以在定植过程中成功竞争。变形链球菌产生的细菌素和血链球菌产生的过氧化氢 (H2O2) 已被证明对于两个物种之间的临床相关拮抗作用至关重要。 H2O2 的一个潜在目标是变形链球菌的细胞包膜。在本研究中，研究了变形链球菌在种间竞争过程中细胞包膜相关的真核丝氨酸/苏氨酸蛋白激酶（STPK）的作用。通过 STPK 编码基因与卡那霉素抗性决定簇的同源重组构建了等位基因替换。已经筛选了突变体对细胞包膜应激的敏感性。先前开发的点样测定用于模拟种间竞争。 STPK-突变体表现出对H2O2引起的包膜应激的敏感性增加，并且在种间竞争测定中受到显着更大的抑制。变形链球菌能够感知竞争物种分泌的抗菌化合物，并有可能调整细胞包膜以提高耐药性。
Interspecies interactions of oral streptococci involve the production and excretion of antimicrobial compounds to compete successfully during colonization. Bacteriocin production by Streptococcus mutans and hydrogen peroxide (H2O2) production by Streptococcus sanguinis have been demonstrated as crucial for the clinical relevant antagonism between both species. A potential target of H2O2 is the cell-envelop of S. mutans. In the present study, the role of cell-envelop associated eukaryotic serine/threonine protein kinase (STPK) in S. mutans during interspecies competition has been investigated. Allelic replacements via homologous recombination of the STPK encoding gene with a kanamycin resistant determinant has been constructed. The mutant has been screened for the susceptibility towards cell-envelope stress. A previously developed spotting assay was used to simulate interspecies competition. The STPK- mutant showed an increased susceptibility toward envelop-stress caused by H2O2 and was significantly more inhibited during interspecies competition assays. S. mutans is able to sense antimicrobial compounds excreted by competing species and can potentially adjust the cell-envelop toward an increased resistance.
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影响因子：
4.4
作者：
Krupa A;Srinivasan N
通讯作者：
Srinivasan N
DOI：
10.1073/pnas.152334799
发表时间：
2002-07-23
影响因子：
11.1
作者：
Skaar, EP;Tobiason, DM;Seifert, HS
通讯作者：
Seifert, HS
影响因子：
13.8
作者：
Yeats, C;Finn, RD;Bateman, A
通讯作者：
Bateman, A
影响因子：
3.1
作者：
Echenique, J;Kadioglu, A;Trombe, MC
通讯作者：
Trombe, MC
影响因子：
3.2
作者：
Kreth, Jens;Zhang, Yongshu;Herzberg, Mark C.
通讯作者：
Herzberg, Mark C.