IN SITU SIV-SPECIFIC CD4 T CELL ANALYSIS DURING ACUTE SIV INFECTION

急性 SIV 感染期间 SIV 特异性 CD4 T 细胞原位分析

• 批准号: 7958804
• 负责人: PAMELA J SKINNER
• 金额: $ 19.66万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7958804
• 关键词: Acute; Antigens; Biology; CD4 Positive T Lymphocytes; Collaborations; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; In Situ; Institution; Knowledge; Macaca; Methodology; Methods; Minnesota; Mission; Primates; Reagent; Research; Research Personnel; Resources; SIV; Scientist; Source; Spleen; Staining method; Stains; T-Cell Receptor; Tissues; Transgenic Mice; United States National Institutes of Health; Universities; Virus Diseases; Wisconsin; human disease

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: To target the Wisconsin National Primate Research Center's mission to develop treatments for human disease, generate knowledge of primate biology, and to facilitate the progress of research by providing expertise and resources to external scientists, we are developing a methodology that we will use to determine the abundance and in situ localization of CD4 T cells specific to simian immunodeficiency virus (SIV) in tissues during SIV infection. Results: Although we have not yet received the pilot grant money from the WNPRC for the project, we have nonetheless started to make some progress. In collaboration with Marc Jenkins at the University of Minnesota we are developing methods to stain antigen specific CD4 T cells in spleen tissues from T cell receptor transgenic mice and Nancy Wilson at the WNPRC is making progress developing class II reagents for use in SIV-infected macaques.

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 目的： 针对威斯康星州国家灵长类动物研究中心的使命，开发人类疾病的治疗方法，产生灵长类动物生物学的知识，并通过提供专业知识和资源，以促进研究的进展，外部科学家，我们正在开发一种方法，我们将用于确定的丰度和原位定位的CD 4 T细胞特异性猴免疫缺陷病毒（SIV）在组织中SIV感染。 结果：虽然我们还没有收到WNPRC为该项目提供的试点赠款，但我们已经开始取得一些进展。 与明尼苏达大学的Marc Jenkins合作，我们正在开发对T细胞受体转基因小鼠脾组织中抗原特异性CD 4 T细胞进行染色的方法，WNPRC的Nancy Wilson正在开发用于SIV感染猕猴的II类试剂。