Scientific Core: Flow Cytometry and Sorting Core Facility

科学核心：流式细胞术和分选核心设施

• 批准号: 8137859
• 负责人: Paul Wayne Kincade
• 金额: $ 10.01万
• 依托单位: OKLAHOMA MEDICAL RESEARCH FOUNDATION
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8137859
• 关键词: Air; Anthrax disease; Antibodies; Arts; Bacillus anthracis; Bacteria; Biohazardous Substance; Blood specimen; Cell Line; Cell surface; Cells; Color; Companions; Computer software; Core Facility; Cyan Fluorescent Protein; Cytokine Signaling; Data; Deposition; Dyes; Effector Cell; Flow Cytometry; Fluorescein-5-isothiocyanate; Fluorescence Resonance Energy Transfer; Fluorochrome; Gel; Green Fluorescent Proteins; Hand; Human; Human Resources; Immune; In Vitro; Infection; Label; Lasers; Learning; Life; Maintenance; Oklahoma; Optics; Population; Postdoctoral Fellow; Procedures; Reagent; Reproduction spores; Research Personnel; Sample Size; Sampling; Signal Transduction; Signaling Molecule; Soldier; Sorting - Cell Movement; Specimen; Speed; Staining method; Stains; Students; Supervision; Texas red; Training; Vaccinated; Viola; Water; Work; cost; cyanine dye 5; data format; digital; experience; innovation; instrument; magnetic beads; operation; peripheral blood; research study

Investigators in this project will continue to benefit from a state of the art facility, with advanced nstrumentation and highly trained technicians at their disposal. The personnel have unique experience in sorting extremely rare cells to high purity and in obtaining nine color data with small sample sizes. They routinely handle human specimens with biohazard precautions, and can work with spores, live bacteria and bacterial products. There is no equivalent expertise in the State of Oklahoma. Costs are contained in part by OMRF institutional support. Training is another important activity of this core facility. While investigators can simply hand samples to the technicians and receive formatted data, they are encouraged to learn at least how to analyze their own results. Most students and postdoctoral fellows prefer this, and a few have even earned how to sort on the FACSAria with close supervision. Advice on related procedures, such as reagent selection and magnetic bead enrichments is also readily available. Many appropriately labeled antibodies are available to facilitate pilot experiments and encourage innovation.

该项目的研究人员将继续受益于最先进的设施，以及先进的 仪器仪表和训练有素的技术人员可供使用。人员在以下方面拥有独特的经验 对极其稀有的细胞进行高纯度分选，并以小样本量获得九种颜色数据。他们 定期处理人体样本并采取生物危害预防措施，并且可以处理孢子、活细菌和 细菌产品。俄克拉荷马州没有同等的专业知识。成本部分包含在 OMRF 机构支持。培训是该核心设施的另一项重要活动。虽然调查人员可以 只需将样品交给技术人员并接收格式化数据，我们鼓励他们至少学习 如何分析自己的结果。大多数学生和博士后更喜欢这个，有一些甚至 了解如何在密切监督下对 FACSAria 进行排序。试剂等相关程序的建议 选择和磁珠富集也很容易实现。许多适当标记的抗体 可用于促进试点实验并鼓励创新。