Cellular and Molecular Neuroscience Core

细胞和分子神经科学核心

• 批准号: 8257638
• 负责人: John P Svaren
• 金额: $ 31.92万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-26 至 2016-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8257638
• 关键词: Alexander Disease; Astrocytes; Biological Assay; Biomanufacturing; Biotechnology; Cancer Center; Cell Line; Cells; Clinical; Commit; Confocal Microscopy; Core Facility; DNA; DNA Sequence; DNA biosynthesis; Developmental Disabilities; Equipment; Flow Cytometry; Fluorescence Microscopy; Funding; Gel; Generations; Genomics; Gifts; Housing; Human; Human Cell Line; In Situ; Individual; Informatics; Intellectual functioning disability; Laser Scanning Confocal Microscopy; Mass Spectrum Analysis; Mental Retardation and Developmental Disabilities Research Centers; Microscopy; Modeling; Molecular; Molecular and Cellular Biology; Neurons; Neurosciences; Patients; Preclinical Drug Evaluation; Production; Proteomics; Research; Research Personnel; Resources; Retinitis Pigmentosa; Rett Syndrome; Services; Technology; Training; Viral Vector; Vitelliform macular dystrophy; chromatin immunoprecipitation; cost effective; design; induced pluripotent stem cell; interest; new technology; programs; response; small molecule; stem cell biology; two-dimensional

The Cellular and Molecular Neuroscience Core is designed to facilitate the research objectives of the IDDRC and the scientific progress of individual IDDRC projects by providing state-of-the-art equipment, training, and expertise for IDDRC investigators employing, or wishing to employ, cellular and molecular biology in their research programs. In-house equipment includes advanced microscopy (laser scanning confocal and fluorescence microscopy with Stereology), genomic and proteomic analysis both in situ (flow cytometry) and in cell lysates (phosphonmaging and quantitative PCR), and informatics. During the current project period, the Waisman Center committed $500,000 of gift funds to upgrade the Core's existing proteomics and confocal microscopy capabilities. In addition, the CMN Core has well-established partnerships with nearby UW-Madison core facilities (UW-Biotechnology Center and UW-Cancer Center) to offer cost effective, state-of-the-art genomic and proteomic technology, such as 2-dimensional gel analysis, mass spectrometry, DNA sequencing and synthesis, DNA array and SNP analysis, NextGen sequencing, tiled array analysis of chromatin immunoprecipitation (ChIP) assays, and small molecule drug screening. In response to the growing interest for human-derived IDD models, in 2009 we created a new service of the CMN Core (with ARRA support) to generate induced pluripotent stem cells (iPSCs) from individuals with IDD conditions for IDDRC investigators. The IPSC service leverages the IDDRC's long-standing expertise in stem cell biology, access to well characterized patients with IDD (via the Research Participation Core), and clinical biomanufacturing (Waisman Biomanufacturing) for the production of IPSC viral vectors. Using these complementary resources, the iPSC service has successfully created 23 cell lines from patients with IDD including Rett syndrome, Alexander disease, FXS, DS, SMA, retinitis pigmentosa. Best disease, and others, and provided guidance and expertise in differentiating the cell lines into neuronal sub-types and astrocytes.

细胞和分子神经科学核心旨在促进IDDRC的研究目标和个人IDDRC项目的科学进展，为IDDRC研究人员提供最先进的设备，培训和专业知识，或希望在他们的研究计划中使用细胞和分子生物学。内部设备包括先进的显微镜（激光扫描共聚焦和 荧光显微术与体视学）、原位（流式细胞术）和细胞裂解物（磷酸成像和定量PCR）中的基因组和蛋白质组学分析以及信息学。在本项目期间，魏斯曼中心承诺提供50万美元的赠款，用于升级核心现有的蛋白质组学和共聚焦显微镜功能。此外，CMN核心与附近的威斯康星大学麦迪逊分校建立了良好的合作关系 UW的核心设施（UW生物技术中心和UW癌症中心）提供具有成本效益的，最先进的基因组和蛋白质组技术，如二维凝胶分析，质谱，DNA测序和合成，DNA阵列和SNP分析，NextGen测序，染色质免疫沉淀（ChIP）测定的平铺阵列分析和小分子药物筛选。 为了应对对人类来源的IDD模型日益增长的兴趣，2009年，我们创建了CMN Core（在ARRA的支持下）的新服务，为IDDRC研究人员从IDD患者中产生诱导多能干细胞（iPSC）。IPSC服务利用IDDRC在干细胞生物学方面的长期专业知识，访问具有良好特征的IDD患者（通过研究参与核心），以及临床 生物制造（Waisman Biomanufacturing）用于生产IPSC病毒载体。利用这些互补资源，iPSC服务已经成功地从IDD患者中创建了23个细胞系，包括Rett综合征，亚历山大病，FXS，DS，SMA，视网膜色素变性。最好的疾病，和其他人，并提供了指导和专业知识，在分化成神经元亚型和星形胶质细胞的细胞系。