Cellular and Molecular Neuroscience Core

细胞和分子神经科学核心旨在通过为在其研究计划中使用或希望使用细胞和分子生物学的IDDRC调查人员提供最先进的设备、培训和专业知识，来促进IDDRC的研究目标和各个IDDRC项目的科学进步。内部设备包括先进的显微镜(激光扫描共聚焦和 荧光显微镜和体视学)，基因组和蛋白质组原位分析(流式细胞术)和细胞裂解产物(磷光成像和定量聚合酶链式反应)，以及信息学。在目前的项目期间，韦斯曼中心承诺提供50万美元的捐赠资金，以升级Core现有的蛋白质组学和共聚焦显微镜能力。此外，CMN Core与附近的UW-Madison建立了良好的合作伙伴关系 核心设施(UW-生物技术中心和UW-癌症中心)，提供具有成本效益的最先进的基因组和蛋白质组技术，如二维凝胶分析、质谱仪、DNA测序和合成、DNA阵列和SNP分析、NextGen测序、染色质免疫沉淀(CHIP)分析的平铺阵列分析和小分子药物筛选。 为了应对人们对人类IDD模型日益增长的兴趣，我们在2009年创建了CMN Core的一项新服务(在ARRA的支持下)，为IDDRC研究人员从患有IDD的个体中培养诱导多能干细胞(IPSCs)。IPSC服务利用了IDDRC在干细胞生物学、接触特征良好的IDD患者(通过研究参与核心)和临床方面的长期专业知识 用于生产IPSC病毒载体的生物制造(Waisman Biomanufacing)。利用这些互补资源，IPSC服务已成功地从IDD患者中创建了23个细胞系，包括Rett综合征、Alexander病、FXS、DS、SMA、视网膜色素变性。BEST疾病和其他疾病，并在区分神经细胞亚型和星形胶质细胞方面提供指导和专业知识。

The Cellular and Molecular Neuroscience Core is designed to facilitate the research objectives of the IDDRC and the scientific progress of individual IDDRC projects by providing state-of-the-art equipment, training, and expertise for IDDRC investigators employing, or wishing to employ, cellular and molecular biology in their research programs. In-house equipment includes advanced microscopy (laser scanning confocal and fluorescence microscopy with Stereology), genomic and proteomic analysis both in situ (flow cytometry) and in cell lysates (phosphonmaging and quantitative PCR), and informatics. During the current project period, the Waisman Center committed $500,000 of gift funds to upgrade the Core's existing proteomics and confocal microscopy capabilities. In addition, the CMN Core has well-established partnerships with nearby UW-Madison core facilities (UW-Biotechnology Center and UW-Cancer Center) to offer cost effective, state-of-the-art genomic and proteomic technology, such as 2-dimensional gel analysis, mass spectrometry, DNA sequencing and synthesis, DNA array and SNP analysis, NextGen sequencing, tiled array analysis of chromatin immunoprecipitation (ChIP) assays, and small molecule drug screening. In response to the growing interest for human-derived IDD models, in 2009 we created a new service of the CMN Core (with ARRA support) to generate induced pluripotent stem cells (iPSCs) from individuals with IDD conditions for IDDRC investigators. The IPSC service leverages the IDDRC's long-standing expertise in stem cell biology, access to well characterized patients with IDD (via the Research Participation Core), and clinical biomanufacturing (Waisman Biomanufacturing) for the production of IPSC viral vectors. Using these complementary resources, the iPSC service has successfully created 23 cell lines from patients with IDD including Rett syndrome, Alexander disease, FXS, DS, SMA, retinitis pigmentosa. Best disease, and others, and provided guidance and expertise in differentiating the cell lines into neuronal sub-types and astrocytes.