ULTRA-SENSITIVE MRI PROBES

超灵敏 MRI 探头

• 批准号: 8363911
• 负责人: Jinming Gao
• 金额: $ 1.61万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363911
• 关键词: Cells; Contrast Media; Detection; Development; Drug Formulations; Funding; Gadolinium DTPA; Grant; Image; Magnetic Resonance Imaging; Malignant Neoplasms; Metabolism; Metals; Methods; Micelles; Molecular; Molecular Weight; Monitor; National Center for Research Resources; Polymers; Postdoctoral Fellow; Principal Investigator; Process; Research; Research Infrastructure; Research Project Grants; Resources; Scanning; Signal Transduction; Solid Neoplasm; Source; Specificity; Training; United States National Institutes of Health; Water; Weight; base; cost; in vivo; iron oxide; molecular imaging; nanoparticle; novel; particle

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The general objective of this research project is to develop ultra-sensitive MRI probes. One approach is based on MFe2O4 (M=Fe, Co Mn)-loaded polymer micelles for cancer molecular imaging applications. The low sensitivity of conventional MR contrast agents severely limits their use for monitoring molecular processes in vivo. Another approach is to used superparamagnetic iron oxide nanoparticles (SPIO). The growing use of SPIO in molecular imaging, cell tracking and biomolecular detection has resulted in the rapid development of novel SPIO formulations and imaging acquisition methods. Compared to the low molecular weight, paramagnetic metal chelates such as Gd-DTPA (T1 contrast agent), SPIO nanoparticles are considered T2-negative contrast agents with substantially higher T1 and T2 relaxivity. Conventionally, SPIO particles are evaluated by T2- or T2*-weighted MR imaging for their abilities in dephasing water signals. Image void at high SPIO concentrations and requirement of a pre-contrast scan limit the accuracy and specificity for SPIO detection. A recent objective is to develop and apply an off-resonance saturation (ORS) method to allow for on-and-off imaging of ¿v¿3-targeted SPIO-loaded polymeric micelles for molecular imaging of solid tumors. The RR has provided access to the 4.7 T, the workhorse for us. Iniitially the imaging was performed by Dr. Jimin Ren, supported by the Research Resource. Dr. Ren trained two post - doctoral fellows, Drs. Charlie Khemtong and Chase Kessinger, and now all imaging is performed by Dr. Gao's team.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 该研究项目的总体目标是开发超灵敏的 MRI 探头。 一种方法是基于 MFe2O4 (M=Fe, Co Mn) 负载的聚合物胶束，用于癌症分子成像应用。 传统磁共振造影剂的低灵敏度严重限制了它们在监测体内分子过程中的应用。 另一种方法是使用超顺磁性氧化铁纳米粒子（SPIO）。 SPIO 在分子成像、细胞追踪和生物分子检测中的应用日益广泛，导致新型 SPIO 配方和成像采集方法的快速发展。 与低分子量顺磁性金属螯合物（如 Gd-DTPA）（T1 造影剂）相比，SPIO 纳米粒子被认为是 T2 阴性造影剂，具有更高的 T1 和 T2 弛豫率。 传统上，SPIO 颗粒通过 T2 或 T2* 加权 MR 成像来评估其对水信号移相的能力。 高 SPIO 浓度下的图像空白和预对比扫描的要求限制了 SPIO 检测的准确性和特异性。 最近的一个目标是开发和应用非共振饱和（ORS）方法，以允许对“v”3靶向的SPIO负载的聚合物胶束进行断断续续的成像，以用于实体瘤的分子成像。 RR 为我们提供了使用主力 4.7 T 的机会。最初，成像是由 Jimin Ren 博士在研究资源的支持下进行的。任博士培养了两名博士后。 Charlie Khemtong 和 Chase Kessinger，现在所有成像都由高博士的团队进行。