Flow Cytometry

项目总结（见说明）; 流式细胞仪共享服务（FCSS）在马里兰州大学玛琳和斯图尔特Greenebaum癌症中心（UMCCC）提供流式细胞仪设备和技术专业知识，以UMCCC成员进行癌症生物学和基础和应用科学的其他领域的研究。服务对整个马里兰州校园开放，但UMCCC占总使用量的75%左右。FCSS提供全面的、最先进的流式细胞术服务，从功能和表型分析到细胞分选。分析仪器包括一台现代化的全数字化3激光Becton Dickinson（BD）LSR II流式细胞仪和两台模拟流式细胞仪：BD LSR 1和BD FACScan仪器。使用（1）配备有两个激光器和七色检测器的BD FACSAria I数字仪器进行细胞分选 能力和（2）BD FACSVantage SE仪器升级BD的数字采集系统（DIVA），并配备了三个激光器，包括可调谐氪激光器的351 nm紫外激发。 FCSS还在多个平台上为用户提供操作员辅助和自助数据分析，以及实验培训和咨询，以支持单个分析或分选实验的项目设计。 FCSS的有效性从近年来其使用率的稳步增长，使用共享服务的50多名主要研究者，以及在共享服务的帮助下进行的实验所支持的出版物和国家癌症研究所资助的项目的数量中显而易见。 FCSS的目标是通过扩大细胞分选能力来进一步扩大其服务的广度和范围;支持基于流式细胞术的新型分析应用;加强与其他UMCCC共享服务的合作，以帮助向UMCCC研究人员介绍创新的尖端技术。

PROJECT SUMMARY (See instructions); The Flow Cytometry Shared Service (FCSS) at the University of Maryland Marlene and Stewart Greenebaum Cancer Center (UMGCC) offers flow cytometry equipment and technical expertise to UMGCC members conducting research in cancer biology and other areas of basic and applied science. Services are open to the entire University of Maryland campus, but UMGCC accounts for about 75 percent of the total use. FCSS provides full scale, state-of-the-art flow cytometry services, from functional and phenotypic analysis to cell sorting. Analytical instruments include a modern, fully digital, 3-laser Becton Dickinson (BD) LSR II flow cytometer, and two analog flow cytometers: a BD LSR 1 and a BD FACScan instrument. Cell sorting is conducted with (1) a BD FACSAria I digital instrument equipped with two lasers and seven-colordetection capability and (2) a BD FACSVantage SE instrument upgraded with BD's Digital Acquisition System (DIVA) and equipped with three lasers including a tunable krypton laser for 351 nm ultraviolet excitation. FCSS also offers users both operator-assisted and self-service data analysis on several platforms as well as experimental training and consultation to support project design for individual analytical or sorting experiments. The effectiveness of FCSS is evident from the steady increase of its utilization in recent years, the more than 50 principal investigators who are using the shared service, and the number of publications and National Cancer Institute-funded projects supported by experiments conducted with the help ofthe shared service. The goal of FCSS is to further increase the breadth and scope of its services by expanding cell-sorting capabilities; supporting novel, flow cytometry-based analytical applications; and strengthening cooperation with other UMGCC shared sen/ices to help introduce innovative, cutting-edge technologies to UMGCC investigators.