Shared Resources - Gene Transfer Vector Core

项目总结(见说明)： 基因转移载体核心被整合到多个癌症项目中，旨在研究疾病过程、治疗和疫苗的开发。该公司的核心员工积极参与癌症领域基因转移技术的开发。与来自不同学科的多个研究人员的互动允许思想、技术进步和载体创新的交叉滋养 设计。载体核心的总体目标是支持研究人员使用基因转移技术，包括咨询PI和工作人员，开发新的载体，为功能和纯度产生的载体进行协作测试，最后是常规准备工作，包括质量控制。病媒核心工作人员和调查人员在病媒设计和生成的所有阶段都保持密切联系，以及 既是基因转移研究的研究和开发设施，也是常规载体准备的服务设施。作为这项服务的一部分，载体核心将提供重组腺病毒、腺相关病毒(AAV)、牛痘病毒、杆状病毒和逆转录病毒(包括慢病毒)的纯化和浓缩制剂。这一设施提供了获得标准细胞系、表达质粒和 重组报告病毒。 核心的主要职责将是：准备重组载体；质量控制；媒介传播；维护可供使用的媒介库存数据库；编目表达载体的质粒库；根据需要开发新的表达载体；开发新的病毒生产方法；协助设计和开发新的载体。 这一设施满足了众多对基因转移载体的基础和应用研究感兴趣的外部研究人员的需求，其重要性有几个原因。首先，通过与许多不同的基因转移载体、方法和应用保持同步，增强了载体核心工作人员的科学专业知识。第二，为更广泛的用户提供服务改善和促进了校际交流 重点致力于开发改进的病媒和投递方法。最后，来自大学内外的一系列新想法，以及通过我们的顾问，确保霍尔顿综合癌症中心社区能够接触到或意识到“最先进的技术”。

PROJECT SUMMARY (See instructions): The Gene Transfer Vector Core is integrated into multiple cancer projects directed at the study of the disease process, therapy and the development of vaccines. Vector Core staff are active participants in the development of gene transfer technologies in the cancer field. The interaction with multiple investigators from various disciplines allows for cross-fertilization of ideas, technical advancements, and innovations in vector designs. The Vector Core's overall objective is to support investigators in the use of gene transfer technologies, including consultation with the PI and staff, development of novel vectors, collaborative testing of vectors generated for function and purity, and finally routine preparation including quality control. Vector Core staff and investigators are in close contact through all phases of vector design and generation, and serves as both a research and development facility for gene transfer studies, and a service facility for routine vector preparations. As a part of the service the Vector Core will provide purified and concentrated preparations of recombinant adenovirus, adeno-associated virus (AAV), vaccinia, baculovirus and retrovirus (including lentivirus). This facility provides access to standard cell lines, expression plasmids, and stock of recombinant reporter viruses. The main responsibilities of the Core will be: Prepare recombinant vectors; Quality control; Vector dissemination; Maintain a database of vector stocks available for use; Catalogue plasmid database of expression vectors; develop new expression vectors as needed; Develop novel methods for virus production; Assist in the design and development of novel vectors. This facility serves the needs of numerous outside investigators interested in both basic and applied research with gene transfer vectors, and is important for several reasons. First, by keeping abreast of many different gene transfer vectors, approaches, and applications, the scientific expertise of the Vector Core staff is strengthened. Second, serving a broad scope of users improves and fosters inter-collegiate communication with focused efforts at developing improved vectors and delivery methods. Finally, a continuum of new ideas from both outside and within the university, and through our consultants insures that the Holden Comprehensive Cancer Center community has access to, or is aware of, the 'state of the art'.