Shared Resources - Gene Transfer Vector Core

共享资源-基因转移载体核心

• 批准号: 8340096
• 负责人: George J. Weiner
• 金额: $ 8.35万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-19 至 2016-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8340096
• 关键词: Address; Adenoviruses; Applied Research; Baculoviruses; Basic Science; Behavior; Binding; Biological Assay; Cancer Center Support Grant; Cataloging; Catalogs; Cell Line; Collaborations; Communication; Communities; Comprehensive Cancer Center; Consultations; Databases; Dependovirus; Development; Discipline; Disease; Economics; Fertilization; Fostering; Gene Transfer; Generations; Genes; Genetic; Individual; Instruction; Label; Laboratories; Malignant Neoplasms; Methods; Participant; Phase; Plasmids; Play; Preparation; Principal Investigator; Process; Production; Quality Control; Recombinants; Reporter; Research Design; Research Personnel; Resource Sharing; Resources; Retroviridae; Role; Services; Staff Development; Subfamily lentivirinae; Technology; Testing; Training; Universities; Vaccines; Vaccinia; Vaccinium; Virus; Work; anticancer research; cancer cell; carcinogenesis; college; design; expression vector; gene transfer vector; improved; innovation; interest; member; novel; recombinant viral vector; research and development; research study; therapy development; vector; virology

PROJECT SUMMARY (See instructions): The Gene Transfer Vector Core is integrated into multiple cancer projects directed at the study of the disease process, therapy and the development of vaccines. Vector Core staff are active participants in the development of gene transfer technologies in the cancer field. The interaction with multiple investigators from various disciplines allows for cross-fertilization of ideas, technical advancements, and innovations in vector designs. The Vector Core's overall objective is to support investigators in the use of gene transfer technologies, including consultation with the PI and staff, development of novel vectors, collaborative testing of vectors generated for function and purity, and finally routine preparation including quality control. Vector Core staff and investigators are in close contact through all phases of vector design and generation, and serves as both a research and development facility for gene transfer studies, and a service facility for routine vector preparations. As a part of the service the Vector Core will provide purified and concentrated preparations of recombinant adenovirus, adeno-associated virus (AAV), vaccinia, baculovirus and retrovirus (including lentivirus). This facility provides access to standard cell lines, expression plasmids, and stock of recombinant reporter viruses. The main responsibilities of the Core will be: Prepare recombinant vectors; Quality control; Vector dissemination; Maintain a database of vector stocks available for use; Catalogue plasmid database of expression vectors; develop new expression vectors as needed; Develop novel methods for virus production; Assist in the design and development of novel vectors. This facility serves the needs of numerous outside investigators interested in both basic and applied research with gene transfer vectors, and is important for several reasons. First, by keeping abreast of many different gene transfer vectors, approaches, and applications, the scientific expertise of the Vector Core staff is strengthened. Second, serving a broad scope of users improves and fosters inter-collegiate communication with focused efforts at developing improved vectors and delivery methods. Finally, a continuum of new ideas from both outside and within the university, and through our consultants insures that the Holden Comprehensive Cancer Center community has access to, or is aware of, the 'state of the art'.

项目总结（见说明）： 基因转移载体核心被整合到多个癌症项目中，这些项目旨在研究疾病过程、治疗和疫苗开发。Vector Core的工作人员积极参与癌症领域基因转移技术的开发。与来自不同学科的多名研究人员的互动允许在矢量方面的思想，技术进步和创新的交叉施肥 的设计. Vector Core的总体目标是支持研究者使用基因转移技术，包括与PI和工作人员进行咨询，开发新型载体，对所生成的载体进行功能和纯度的协作检测，以及最终的常规制备，包括质量控制。Vector Core工作人员和研究人员在载体设计和生成的所有阶段都保持密切联系， 既是基因转移研究的研发设施，又是常规载体制备的服务设施。作为服务的一部分，Vector Core将提供重组腺病毒、腺相关病毒（AAV）、牛痘、杆状病毒和逆转录病毒（包括慢病毒）的纯化和浓缩制剂。该设施提供了标准细胞系、表达质粒和 重组报告病毒。 核心的主要职责是：制备重组载体;质量控制;载体传播;维护可供使用的载体库存数据库;对表达载体的质粒数据库进行编目;根据需要开发新的表达载体;开发新的病毒生产方法;协助设计和开发新的载体。 该设施满足了许多对基因转移载体的基础和应用研究感兴趣的外部研究人员的需求，并且由于以下几个原因而非常重要。首先，通过了解许多不同的基因转移载体、方法和应用，Vector Core工作人员的科学专业知识得到了加强。第二，服务于广泛的用户，改善和促进校际交流 集中精力开发改进的载体和传递方法。最后，来自大学内外的一系列新想法，并通过我们的顾问确保霍尔顿综合癌症中心社区能够获得或了解“最先进的”。