Structural Biology Core Facility

结构生物学核心设施

• 批准号: 8340313
• 负责人: Matthew R Redinbo
• 金额: $ 22.08万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-23 至 2015-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8340313
• 关键词: Accounting; Address; Antineoplastic Agents; Area; Basic Science; Bioinformatics; Biology; Biomolecular Nuclear Magnetic Resonance; Cancer Center; Cancer Center Support Grant; Chemicals; Complex; Computing Methodologies; Core Facility; Core Protein; Crystallization; Development; Drug Design; Drug Industry; Education; Environment; Equipment; Faculty; Funding; Grant; Homology Modeling; Housing; Human Resources; Lead; Malignant Neoplasms; Mechanics; Molecular Models; NMR Spectroscopy; Nucleic Acids; Peer Review; Pharmaceutical Preparations; Photons; Preparation; Process; Protein Dynamics; Proteins; Publications; Records; Research; Research Infrastructure; Research Personnel; Resolution; Resources; Roentgen Rays; Sampling; Schedule; Scientist; Services; Source; Staging; Structural Biologist; Structure; Synchrotrons; Technology; X-Ray Crystallography; base; cost; design and construction; drug development; drug discovery; experience; inhibitor/antagonist; insight; macromolecule; member; molecular dynamics; molecular modeling; novel; protein complex; protein expression; protein purification; quantum; research study; structural biology; synergism; tool

Structural Biology Core Facility The Structural Biology Core is a 'super core' that provides an integrated platform of expertise, education, and infrastructure to make structural biology available as tools to LCCC researchers. The Core allows engaging in high-resolution studies using X-ray crystallography, multi-dimensional nuclear magnetic resonance spectroscopy and/or computational methods. The Core offers access to equipment for, and expert guidance of users through, all stages of structure determination projects: homology modeling, construct design, protein expression & purification, crystallization, structure determination, structure analysis, biophysical studies, molecular dynamics studies, presentation & publication. The utility of the available resources is demonstrated by numerous structural studies that contribute to the understanding of cancer-related processes at the atomic level and that can be used to develop potential new therapies through structure aided drug design. The Core is led by a team of highly experienced structural biologists with proven track records in cancer-related research In 2009, 32 LCCC members, all peer-reviewed accounted for 82% of total Core use.. With the recruitment of a director for the Core, Dr. Machius, in the summer of 2009, there has been a reorganization of the facilities, together with substantial renovations and equipment acquisition. As a result of the expanded services and increased demand, the number of projects is increasing sharply. Additional personnel are required to fulfill the needs of LCCC members. Renovations are currently underway to consolidate the Structural Biology facilities into contiguous space, providing a single point of access to resources and allowing for fighter integration of equipment and personnel. Also, an efficient interface is being formed between the Structural Biology Core and the Center for Integrative Chemical Biology and Drug Discovery (directed by Dr. Stephen Frye), which will establish a comprehensive pipeline available to LCCC members for the development of novel anti-cancer drugs based on insights gained from structural biology projects. For 2010, the LCCC requests $119,367, an increase of 29% for additional personnel). CCSG funds are projected to be 15% of operating costs.

结构生物学核心设施 结构生物学核心是一个“超级核心”，提供了一个专业知识，教育和基础设施的综合平台，使结构生物学可作为LCCC研究人员的工具。Core允许使用X射线晶体学，多维核磁共振光谱学和/或计算方法进行高分辨率研究。核心提供设备和专家指导 结构测定项目的所有阶段：同源建模、构建体设计、蛋白质表达和纯化、结晶、结构测定、结构分析、生物物理研究、分子动力学研究、演示和出版。许多结构研究证明了可用资源的效用，这些研究有助于理解癌症相关的 这些技术可以在原子水平上进行，并可用于通过结构辅助药物设计开发潜在的新疗法。核心由一组经验丰富的结构生物学家领导，他们在癌症相关研究方面有着良好的记录 2009年，32个LCCC成员，所有同行评审占核心使用总量的82%。随着2009年夏天征聘了核心中心主任Machius博士，对设施进行了重组，并进行了大量翻修和设备购置。由于服务的扩大和需求的增加，项目的数量急剧增加。需要额外的人员来完成 LCCC成员的需求。 目前正在进行翻修，以将结构生物学设施整合到连续的空间中，提供一个获取资源的单一点，并允许战斗机整合设备和人员。此外，结构生物学核心与综合化学生物学和药物发现中心（由Stephen Frye博士指导）之间正在形成一个有效的接口，该接口将建立一个 为LCCC成员提供全面的管道，用于基于结构生物学项目获得的见解开发新型抗癌药物。2010年，LCCC要求119 367美元，增加29%的额外人员）。CCSG资金预计将占运营成本的15%。