Animal Model Research Core

NORC的动物模型研究核心设施是为了促进研究人员的研究工作，这些研究人员致力于研究与肥胖和营养相关的科学问题，这些问题涉及小鼠模型。核心，自十多年前成立以来，一直专注于提供服务，这将提高动物研究的转化潜力，以增加研究活动将导致临床意义的可能性。 结果核心服务包括：提供基因工程小鼠;进行来自小鼠模型的血清和组织的生化测定;免疫细胞化学;进行体内代谢测试（包括代谢率、血压、葡萄糖耐量和胰岛素耐量测试、高胰岛素血症/正常血糖钳夹程序和身体组成测定）;提供用于mRNA测定的设施， 动脉粥样硬化测定;以及提供动物维护和表型分析的培训和咨询。 在最后一个资助期间，核心帮助年轻的研究人员建立了他们在营养和肥胖研究方面的职业生涯，吸引了其他科学学科的高级研究人员进行营养研究，并支持营养研究人员进行具有明确转化潜力的重要观察。这是通过对小鼠血清进行近39，000次生化分析，对组织进行超过3，200次生化分析，通过间接量热法表征278只小鼠，测量血液 在738只小鼠中进行压力测试，在小鼠中进行3，812次身体成分测定，并提供超过100小时的营养相关技术培训等活动。该核心受益于通过与NIDDK资助的华盛顿大学糖尿病研究和培训中心（DRTC）的小鼠表型核心联合运作和大量机构投资所实现的协同效应。在下一个资助期内，Core计划通过持续的服务，培训和支持来保持我们的卓越传统，并通过提供活体动物成像来增强我们的能力， NORC研究人员在肥胖和营养方面进行研究。

The Animal Model Research Core facility of the NORC is to facilitate the research efforts of investigators pursuing scientific problems relevant to obesity and nutrition that involve murine models. The Core, since its inception more than a decade ago, has focused on providing services that will enhance the translational potential of animal research to increase the likelihood that research activities will lead to clinically meaningful results. Core services include: providing genetically engineered mice; performing biochemical assays of serum and tissue from mouse models; imunocytochemistry; performing in vivo metabolic testing (including metabolc rate, blood pressure, glucose tolerance and insulin tolerance testing, hyperinsulinemic/euglycemic clamp procedures, and body composition determinations); providing facilities for mRNA assays and atherosclerosis assays; and providing training and consultation for animal maintenance and phenotyping. During this last funding period, the Core assisted young investigators as they established their careers in nutrition and obesity research, attracted senior investigators from other scientific disciplines to nutrition research, and supported nutritional researchers as they made important observations with clear translational potential. This was accomplished by performing nearly 39,000 biochemical assays of mouse serum, over 3,200 biochemical assays of tissues, characterizing 278 mice by indirect calorimetry, measuring blood pressure in 738 mice, performing 3,812 body composition determinations in mice, and providing over 100 clock hours of training in nutrition-related techniques, among other activities. The Core has benefited from synergies achieved through joint operation with the Mouse Phenotyping Core ofthe NlDDK-funded Washington University Diabetes Research and Training Center (DRTC) and substantial institutional investments. In the next funding period, the Core plans to maintain our tradition of excellence through continued service, training and support, and to enhance our capabilities by offering in vivo animal imaging to NORC investigators performing research in obesity and nutrition.