Animal Model Research Core

NORC的动物模型研究核心设施是为了促进研究人员的研究工作，这些研究人员正在寻求与肥胖和营养相关的科学问题，涉及小鼠模型。自十多年前成立以来，Core一直专注于提供服务，以增强动物研究的翻译潜力，以增加研究活动将导致具有临床意义的 结果。核心服务包括：提供基因工程小鼠；对小鼠模型的血清和组织进行生化分析；免疫细胞化学；进行体内代谢测试(包括代谢率、血压、葡萄糖耐量和胰岛素耐量测试、高胰岛素/正血糖钳夹程序和身体成分测定)；提供信使核糖核酸分析和人体成分测定的设施 动脉粥样硬化检测；以及为动物维护和表型鉴定提供培训和咨询。 在最后一次资助期间，核心帮助年轻研究人员在营养和肥胖研究领域建立了自己的职业生涯，吸引了来自其他科学学科的资深研究人员从事营养研究，并支持营养研究人员进行具有明显翻译潜力的重要观察。这是通过对小鼠血清进行近39,000次生化分析，对组织进行超过3,200次生化分析，通过间接量热法确定278只小鼠的特征，测量血液 该中心为738只小鼠提供压力测试，对小鼠进行3,812次身体成分测定，并提供超过100个小时的营养相关技术培训，以及其他活动。核心受益于通过与NlDDK资助的华盛顿大学糖尿病研究和培训中心(DRTC)的小鼠表型核心的联合运作和大量机构投资而实现的协同效应。在下一个资助期，核心计划通过持续的服务、培训和支持来保持我们卓越的传统，并通过提供活体动物成像来增强我们的能力 NORC的研究人员正在进行肥胖和营养方面的研究。

The Animal Model Research Core facility of the NORC is to facilitate the research efforts of investigators pursuing scientific problems relevant to obesity and nutrition that involve murine models. The Core, since its inception more than a decade ago, has focused on providing services that will enhance the translational potential of animal research to increase the likelihood that research activities will lead to clinically meaningful results. Core services include: providing genetically engineered mice; performing biochemical assays of serum and tissue from mouse models; imunocytochemistry; performing in vivo metabolic testing (including metabolc rate, blood pressure, glucose tolerance and insulin tolerance testing, hyperinsulinemic/euglycemic clamp procedures, and body composition determinations); providing facilities for mRNA assays and atherosclerosis assays; and providing training and consultation for animal maintenance and phenotyping. During this last funding period, the Core assisted young investigators as they established their careers in nutrition and obesity research, attracted senior investigators from other scientific disciplines to nutrition research, and supported nutritional researchers as they made important observations with clear translational potential. This was accomplished by performing nearly 39,000 biochemical assays of mouse serum, over 3,200 biochemical assays of tissues, characterizing 278 mice by indirect calorimetry, measuring blood pressure in 738 mice, performing 3,812 body composition determinations in mice, and providing over 100 clock hours of training in nutrition-related techniques, among other activities. The Core has benefited from synergies achieved through joint operation with the Mouse Phenotyping Core ofthe NlDDK-funded Washington University Diabetes Research and Training Center (DRTC) and substantial institutional investments. In the next funding period, the Core plans to maintain our tradition of excellence through continued service, training and support, and to enhance our capabilities by offering in vivo animal imaging to NORC investigators performing research in obesity and nutrition.