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Proteomics Core

蛋白质组学核心

基本信息

批准号：
8257682
负责人：
Judith A Steen
金额：
$ 14.79万
依托单位：
BOSTON CHILDREN'S HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8257682
关键词：
Acetylation Acylation Address Area Bioinformatics Brain Cell physiology Cells Cellular biology Child Co-Immunoprecipitations Commit Complex Mixtures Consultations Development Developmental Disabilities Digestion Disease Ensure Fourier transform ion cyclotron resonance Functional disorder Gel Goals High Pressure Liquid Chromatography Instruction Investigation Isoelectric Focusing Isotopes Laboratories Lead Mass Spectrum Analysis Measures Mental Retardation and Developmental Disabilities Research Centers Methodology Methods Methylation Modification Molecular Molecular Biology Molecular Weight Nervous system structure Neurobiology Neurodevelopmental Disability Neurons Pediatric Hospitals Peptides Phosphorylation Post-Translational Protein Processing Preparation Protein Analysis Protein Biochemistry Proteins Proteome Proteomics Protocols documentation Recruitment Activity Research Research Personnel Running Sampling Services Solutions Statistical Data Interpretation Structure Subcellular Fractions Subgroup Techniques Technology Testing Tissues base comparative design genome sequencing glycosylation interest liquid chromatography mass spectrometry medical specialties member professor programs protein complex protein expression protein function research study success tool ultra high resolution

项目摘要

B1. OBJECTIVE The Proteomics Core aims to provide all investigators at the Children's/Harvard IDDRC with access to the state-of-the-art mass spectrometry-based proteomics required to advance studies in the cell and molecular biology of neurodevelopmental disabilities. This core provides instruction, consultation and service for the analysis of proteins by mass spectrometry, including protein identification, characterization of post-translational modifications, and quantification of proteins in samples with a wide range of complexities. Overview. Mass spectrometry-based proteomics is currently the most sensitive, quantitative and comprehensive technology available for the characterization of proteins. A major goal of Core B is to use proteomics to assist IDDRC investigators in the identification of protein complexes relevant to neuronal function, to measure the quantitative changes in protein composition that occur in the nervous system during development and under conditions that lead to developmental disabilities, and to identify functional posttranslational modifications of neuronal proteins. Proteomics is a rapidly progressing field and the Core is committed to introducing and implementing new methodology and applications as they become available. Dr. Steen, an Assistant Professor in the Neurobiology Program at Children's Hospital, and an expert in protein biochemistry and mass spectrometry, was recruited to setup and now act as director of the IDDRC Proteomics Core located within the Kirby Neurobiology Center at Children's. The Steen Laboratory is actively involved in proteomics research and has a computational/bioinformatics subgroup, which develops proteomics related statistical and data analysis tools and provides access to this statistical and computational expertise via the Core to all members of the IDDRC. The Core provides advice and the technology for protein quantification and comparative proteomics to IDDRC members. This Core has been designed to provide qualitative and quantitative mass spectrometry-based proteomics technologies. Since the inception of the Proteomics Core in 2005, these powerful proteomic technologies have been successfully used in many IDDRC laboratories for the proteomic analysis of cells and tissues to study intellectual and developmental diseases. The application of proteomic techniques to the study of the developing brain is poised to accomplish i) characterization of the full constellation of proteins in defined cells and tissues, ii) an assessment of changes in protein content or modifications as a function of disease or change in cell state, and iii) characterization of protein function. These investigations involve both large scale and directed proteomic experiments. Large scale studies are often quantitative, involving isotope tagging methods to find differentially expressed or modified proteins; whereas directed studies examine the structure of a single protein including its co-translational and post-translational modifications with respect to the protein's function. The proteomics Core provides services which encompass both these areas. Genome sequencing has provided the opportunity to address questions regarding brain development, disease and dysfunction using approaches previously impossible. To understand the molecular mechanisms of intellectual development and pathophysiology, IDDRC investigators can now directly interrogate the "proteome" of cells or tissues. This Core will provide a critical interactive center for IDDRC investigators by providing protein identification services, post-translational modification analyses and protein quantification.

B1。客观的蛋白质组学核心旨在为儿童/哈佛大学 IDDRC 的所有研究人员提供先进的基于质谱的蛋白质组学，以推进神经发育障碍的细胞和分子生物学研究。该核心为通过质谱分析蛋白质提供指导、咨询和服务，包括蛋白质鉴定、翻译后修饰的表征以及各种复杂样品中蛋白质的定量。概述。基于质谱的蛋白质组学是目前用于蛋白质表征的最灵敏、定量和全面的技术。 Core B 的一个主要目标是利用蛋白质组学协助 IDDRC 研究人员鉴定与神经元功能相关的蛋白质复合物，测量神经系统在发育过程中和导致发育障碍的条件下发生的蛋白质组成的定量变化，并鉴定神经元蛋白质的功能性翻译后修饰。蛋白质组学是一个快速发展的领域，核心致力于引入和实施新的方法和应用程序。 Steen 博士是儿童医院神经生物学项目的助理教授，也是蛋白质生物化学和质谱分析方面的专家，他被招募来建立位于儿童医院柯比神经生物学中心的 IDDRC 蛋白质组学核心，并担任主任。 Steen 实验室积极参与蛋白质组学研究，并拥有一个计算/生物信息学小组，该小组开发蛋白质组学相关的统计和数据分析工具，并通过核心向 IDDRC 的所有成员提供统计和计算专业知识。核心为 IDDRC 成员提供蛋白质定量和比较蛋白质组学的建议和技术。该核心旨在提供基于质谱的定性和定量蛋白质组学技术。自 2005 年蛋白质组学核心启动以来，这些强大的蛋白质组学技术已成功应用于许多 IDDRC 实验室，用于细胞和组织的蛋白质组学分析，以研究智力和发育疾病。蛋白质组学技术在发育中大脑研究中的应用有望实现 i) 确定细胞和组织中全部蛋白质的表征，ii) 评估蛋白质含量或修饰随疾病或细胞状态变化的变化，以及 iii) 蛋白质功能的表征。这些研究涉及大规模和定向蛋白质组学实验。大规模研究通常是定量的，涉及同位素标记方法以发现差异表达或修饰的蛋白质；而定向研究则检查单个蛋白质的结构，包括其与蛋白质功能相关的共翻译和翻译后修饰。蛋白质组学核心提供涵盖这两个领域的服务。基因组测序为利用以前不可能的方法解决有关大脑发育、疾病和功能障碍的问题提供了机会。为了了解智力发育和病理生理学的分子机制，IDDRC 研究人员现在可以直接询问细胞或组织的“蛋白质组”。该核心将为 IDDRC 研究人员提供一个重要的互动中心，提供蛋白质鉴定服务、翻译后修饰分析和蛋白质定量。