BD Influx 5 Laser Fluorescence Activated Cell Sorter

描述（由申请人提供）：我们正在申请资金，以支持购买必要的仪器，以满足塔夫茨流式细胞术核心的需求。这个核心设施提供了唯一的细胞分选能力，为整个扩大塔夫茨研究社区 包括塔夫茨大学医学、牙科、兽医和工程学院以及塔夫茨-新英格兰医学中心和圣伊丽莎白医院。目前，这包括47个积极使用细胞分选的生物医学研究实验室，其中31个由NIH资助。这些包括在广泛的健康和疾病相关领域工作的研究人员，包括免疫学，癌症，微生物学，疾病发病机制和组织工程。该设施已运行了15年，由艾伦帕米利先生，一个熟练的和高度尊重的操作员，在私人侦探的直接监督下。核心咨询委员会。在他们的指导下，该设施不断发展，取得了巨大成功，并通过有竞争力的定价和部门支持保持了财务稳定。90%的设施时间用于主要用户组的NIH资助工作。我们现在需要一台带有5个激光器的Influx细胞分选仪（BD Biosciences）。我们目前的细胞分选机（12年和13年的MoFlos）已经过时，容易发生故障，只有一台具有5激光，4路分选能力。我们预计这些老化的机器将变得越来越不可靠，最迟到2013年，我们的机器将不再得到零件支持。因此，我们目前的分类能力很快就会丧失。因此，要保证细胞分选服务的连续性，就需要在2013年增加一台新机器，至少要与我们目前的配置能力相匹配。新的Influx细胞分选机不仅提供更高的可靠性，而且与我们现有的MoFlo相比，它还具有重大的技术优势。这些包括探测器数量的急剧增加和5个独立的针孔。该仪器将有效地取代我们的5激光MoFlo，同时提供一系列我们目前无法获得的技术，例如使用某些染料和荧光蛋白配置，这些配置在高斯托克斯位移下工作（例如，某些转移染料和FRET系统）。我们设施的成本效益和高标准确保了细胞分选机的稳定使用，随着核心被纳入大学范围的计划，以开发大规模，多名研究人员，由最先进的设施和核心支持的研究合作，细胞分选机的使用将增加。

DESCRIPTION (provided by applicant): We are applying for funds to support the purchase of instrumentation necessary to serve the needs of the Tufts Flow Cytometry Core. This Core Facility provides the only cell sorting capability for the entire extended Tufts research community covering the Tufts University Medical, Dental, Veterinary and Engineering Schools as well as the Tufts-New England Medical Center and St Elizabeth's Hospital. Currently this includes 47 biomedical research laboratories that actively use cell sorting of which 31 are NIH funded. These include investigators working in a broad range of health and disease related areas including immunology, cancer, microbiology, disease pathogenesis and tissue engineering. The facility has been run for the last 15 years by Mr. Allen Parmelee, a skilled and highly respected operator, under the direct supervision of the P.I. and the Core Advisory Committee. Under their direction the facility has grown, become highly successful and maintained financial stability through competitive pricing and Departmental support. 90% of facility time is dedicated to the NIH funded work of the Major User Group. We are now asking for an Influx cell sorter with 5 lasers (BD Biosciences). Our current cell sorters (12 and 13 year old MoFlos) are outdated, prone to breaking down and only one has 5 laser, 4-way sorting capability. We expect these ageing machines to become increasingly unreliable and by 2013, at the latest, our machines will no longer be supported for parts. Consequently our current sorting capability will soon be lost. Guaranteed continuity of the cell sorting service therefore requires the addition of a new machine in 2013 that at least matches the capabilities of our current configuration. Not only will a new Influx cell sorter provide increased reliability, it also possesses major technical advantages over our existing MoFlo. These include a dramatic increase in the number of detectors and 5 independent pinholes. This instrument will effectively replace our 5 laser MoFlo while providing access to a range of techniques not currently available to us such as the use of certain dye and fluorescent protein configurations that operate at high Stokes shifts (e.g., certai transfer dyes and FRET systems). The cost efficiency and high standards of our facility have ensured steady use of the cell sorters which will increase as the core becomes folded into University wide plans to develop large scale, multiple investigator, research collaborations supported by state of the art facilities and cores.