FLOW CYTOMETERS FOR ANALYSIS (FACSCALIBUR) AND SORTING: AGING, LYME ARTHRITIS

用于分析（FACSCALIBUR）和分类的流式细胞仪：老化、莱姆关节炎

• 批准号: 7335013
• 负责人: David A. Thorley-Lawson
• 金额: $ 3.07万
• 依托单位: TUFTS UNIVERSITY BOSTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335013
• 关键词: Lyme disease; community; flow cytometry; lasers; measurement

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): We are applying for funds to support the purchase of instrumentation, necessary to serve the flow cytometry needs of the research community at Tufts University School of Medicine (TUSM) and Tufts-New England Medical Center (T-NEMC). The facility has been in existence for 17 years and has been run for the last 6 years by Mr. Allen Parmelee, a skilled and respected operator, under the direct supervision of the P.I. and the Core Advisory Committee. Under their direction the facility has grown, become highly successful and maintained financial stability through competitive pricing and Departmental support. 85% of facility time is dedicated to the NIH funded work of the Core User Group. We are now asking for an LSR II (BD Biosciences) analytical machine. This instrument will double our analytical capacity, while providing access to a powerful range of techniques not currently available to us, including the measurement of intracellular calcium in cells expressing GFP variants, using Indo-1 (355nm laser); the tracking of cell cycle, viability, and proliferation, using Hoechst or DAPI (355nm laser); the analysis of protein expression, using CFP (405nm laser); the measurement of molecular association at the nanometer scale, using CFP-to-YFP FRET (405nm laser) and any analysis requiring 5 or more colors. The cost efficiency and high standards of our facility have encouraged use to the point where the facility (one FACSCalibur and one, soon to be two, MoFLos) is now over-extended. In addition, new faculty hiring, combined with growing interest in flow applications throughout TUSM and, recently, T-NEMC has increased the size of our NIH funded Core User Group from 6 to 17. This renders the acquisition of a new LSR II essential, if we are to meet the flow cytometry needs, both in time and available technology, for the community of NIH funded researchers at TUSM and T-NEMC.

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。描述（由申请人提供）：我们正在申请资金，以支持仪器的购买，必要的服务于塔夫茨大学医学院（TUSM）和塔夫茨新英格兰医学中心（T-NEMC）的研究社区的流式细胞术的需求。该设施已经存在了17年，并已运行了6年，由艾伦Parmelee先生，一个熟练的和受人尊敬的操作员，直接监督下的PI。核心咨询委员会。在他们的指导下，该设施不断发展，取得了巨大成功，并通过有竞争力的定价和部门支持保持了财务稳定。85%的设施时间用于核心用户组的NIH资助工作。我们现在需要LSR II（BD Biosciences）分析仪。该仪器将使我们的分析能力增加一倍，同时提供了一系列我们目前无法获得的强大技术，包括使用Indo-1测量表达GFP变体的细胞中的细胞内钙。（355 nm激光）;使用Hoechst或DAPI跟踪细胞周期、活力和增殖使用CFP（405 nm激光）分析蛋白质表达;使用CFP-to-YFP FRET（405 nm激光）测量纳米尺度的分子缔合以及需要5种或更多种颜色的任何分析。我们的设施的成本效益和高标准鼓励使用的点，该设施（一个FACSCalibur和一个，很快将是两个，MoFLos）现在过度扩展。此外，新的教师招聘，加上整个TUSM和最近T-NEMC对流量应用的兴趣日益增长，我们的NIH资助的核心用户组的规模从6增加到17。这使得购买新的LSR II至关重要，如果我们要满足TUSM和T-NEMC的NIH资助研究人员社区在时间和可用技术方面的流式细胞术需求。