FLOW CYTOMETERS FOR ANALYSIS (FACSCALIBUR) AND SORTING: AGING, LYME ARTHRITIS

用于分析（FACSCALIBUR）和分类的流式细胞仪：老化、莱姆关节炎

• 批准号: 7335013
• 负责人: David A. Thorley-Lawson
• 金额: $ 3.07万
• 依托单位: TUFTS UNIVERSITY BOSTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335013
• 关键词: Lyme disease; community; flow cytometry; lasers; measurement

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): We are applying for funds to support the purchase of instrumentation, necessary to serve the flow cytometry needs of the research community at Tufts University School of Medicine (TUSM) and Tufts-New England Medical Center (T-NEMC). The facility has been in existence for 17 years and has been run for the last 6 years by Mr. Allen Parmelee, a skilled and respected operator, under the direct supervision of the P.I. and the Core Advisory Committee. Under their direction the facility has grown, become highly successful and maintained financial stability through competitive pricing and Departmental support. 85% of facility time is dedicated to the NIH funded work of the Core User Group. We are now asking for an LSR II (BD Biosciences) analytical machine. This instrument will double our analytical capacity, while providing access to a powerful range of techniques not currently available to us, including the measurement of intracellular calcium in cells expressing GFP variants, using Indo-1 (355nm laser); the tracking of cell cycle, viability, and proliferation, using Hoechst or DAPI (355nm laser); the analysis of protein expression, using CFP (405nm laser); the measurement of molecular association at the nanometer scale, using CFP-to-YFP FRET (405nm laser) and any analysis requiring 5 or more colors. The cost efficiency and high standards of our facility have encouraged use to the point where the facility (one FACSCalibur and one, soon to be two, MoFLos) is now over-extended. In addition, new faculty hiring, combined with growing interest in flow applications throughout TUSM and, recently, T-NEMC has increased the size of our NIH funded Core User Group from 6 to 17. This renders the acquisition of a new LSR II essential, if we are to meet the flow cytometry needs, both in time and available technology, for the community of NIH funded researchers at TUSM and T-NEMC.

该子项目是利用 NIH/NCRR 资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：我们正在申请资金来支持购买仪器，以满足塔夫茨大学医学院 (TUSM) 和塔夫茨新英格兰医学中心 (T-NEMC) 研究界的流式细胞术需求。该设施已存在 17 年，过去 6 年一直由熟练且受人尊敬的操作员 Allen Parmelee 先生在 P.I. 的直接监督下运营。和核心顾问委员会。在他们的指导下，该设施不断发展壮大，取得了巨大成功，并通过有竞争力的价格和部门支持保持了财务稳定。 85% 的设施时间专门用于 NIH 资助的核心用户组工作。我们现在需要一台 LSR II (BD Biosciences) 分析机。该仪器将使我们的分析能力加倍，同时提供我们目前无法使用的一系列强大技术，包括使用 Indo-1（355nm 激光）测量表达 GFP 变体的细胞中的细胞内钙；使用 Hoechst 或 DAPI（355 nm 激光）跟踪细胞周期、活力和增殖；使用CFP（405nm激光）分析蛋白质表达；使用 CFP-to-YFP FRET（405 nm 激光）测量纳米级分子缔合，以及任何需要 5 种或更多颜色的分析。我们设施的成本效率和高标准鼓励使用，以至于设施（一台 FACSCalibur 和一台，很快将是两台 MoFLos）现在已经过度扩展。此外，新教师的招聘，加上整个 TUSM 以及最近 T-NEMC 对流式应用日益浓厚的兴趣，将 NIH 资助的核心用户组的规模从 6 人增加到 17 人。如果我们要满足 TUSM 和 T-NEMC NIH 资助的研究人员群体在时间和可用技术方面的流式细胞术需求，那么购买新的 LSR II 至关重要。