A 9.4T Magnet for real-time metabolic studies

描述（由申请人提供）：该提案要求提供资金，以支持一种新的、最先进的、高分辨率的400 MHz磁共振光谱仪和成像仪，用于使用超极化碳和氮技术研究代谢途径。拟议的系统最初将由13个NIH资助的项目使用，并由宾夕法尼亚大学、费城儿童医院和Lankenau医学研究所的资深研究人员领导。目前，所提出的资源是进一步进行超极化碳-13技术的临床前细胞培养和动物试验的唯一可行途径。在我们的设施现有的核磁共振光谱仪是超过25岁，最近变得不可靠，除了长期存在的困难与调度和可用性。将具有现代屏蔽的新系统并入专用超极化核心将允许设备在DNP偏振器附近的有利定位，以及所需辅助设备的永久并入。这将使许多研究人员能够在各种不同的研究项目中使用超极化碳-13技术。该仪器对于继续扩大超极化研究是必要的。它将主要用于使用超极化造影剂的体内代谢研究，尽管提出了几种灌注器官、灌注细胞团，这是一个利用DNP信号增强对提取物进行NMR定量的项目。该核心最初将由宾夕法尼亚大学的三个部门，费城儿童医院的两个部门和Lankenau医学研究所使用。我们预计，随着该资源的知名度和地位越来越高，这一数字将大幅增长。该系统将作为共享资源提供给宾夕法尼亚大学的研究人员。将受益的部门包括：放射科、肺科、内科、生物物理和生物工程。目前，这些部门的调查人员之间积极合作，支持了这一点。我们预计，这样的系统将显着提高调查和合作研究相关的新兴和强大的技术超极化碳-13及其在代谢成像和光谱学的应用。如果获得资助，该提案沿着其他已确定的承诺将提供资源，为各个项目描述中概述的调查研究计划提供全面的代谢资源。该系统将放置在宾夕法尼亚大学放射科的设施中并由其维护，并由内部咨询委员会管理和指导，该委员会由PI、合作研究者和主要用户组成。

DESCRIPTION (provided by applicant): This proposal requests funding to support a new, state-of-the-art, high-resolution 400- MHz magnetic resonance spectrometer and imager for studying metabolic pathways using hyperpolarized carbon and nitrogen technology. The proposed system will initially be used by thirteen NIH-funded projects and led by well-established investigators at the University of Pennsylvania, Children Hospital of Philadelphia, and the Lankenau Institute for Medical Research. At present, the proposed resource is the only feasible way to pursue further pre-clinical cell culture and animal testing of hyperpolarized carbon-13 technology. An existing NMR spectrometer at our facility is more than 25 years old, and has recently become unreliable in addition to long-standing difficulties with scheduling and availability. Incorporation of a new system with modern shielding into a dedicated hyperpolarized core will allow favorable positioning of the equipment near a DNP polarizer, and the permanent incorporation of required ancillary equipment. This will make it possible for a number of investigators to use hyperpolarized carbon-13 technology in variety of different research projects. This instrument is necessary for continued expansion of hyperpolarization studies. It will be used primarily for in vivo metabolic studies with hyperpolarized contrast agents, although several perfused organ, perfused cell mass are proposed, as is one project which makes use of DNP signal enhancement for NMR quantification of extracts. The core will be initially used by three departments at the University of Pennsylvania, two departments at the Children Hospital of Philadelphia, and the Lankenau Institute for Medical Research. We expect this number to grow substantially as the resource becomes more widely known and established. The system will be available as a shared resource to investigators at the University of Pennsylvania. Departments that will benefit include: Radiology, Pulmonary, Medicine, Biophysics and Bioengineering. This is supported by currently active collaborations between the investigators from these departments. We expect that such a system will significantly enhance investigational and collaborative research related to the emerging and powerful technology of hyperpolarized carbon-13 and its application in metabolic imaging and spectroscopy. If funded, this proposal, along with the other identified commitments, will provide the resources to make a comprehensive metabolic resource available to the investigative research programs outlined in the descriptions of individual projects. The system will be placed at and maintained by the facilities of the Department of Radiology at the University of Pennsylvania and will be managed and directed by an Internal Advisory Committee composed of the PI, co-investigators, and the principal users.