A 9.4T Magnet for real-time metabolic studies

用于实时代谢研究的 9.4T 磁铁

• 批准号: 8447884
• 负责人: RAHIM R RIZI
• 金额: $ 58.25万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-25 至 2014-12-24
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8447884
• 关键词: 2,4-Dinitrophenol; Advisory Committees; Animal Testing; Biomedical Engineering; Biophysics; Carbon; Cell Culture Techniques; Cells; Collaborations; Contrast Media; Equipment; Funding; Image; Individual; Institutes; Magnetic Resonance; Medical Research; Metabolic; Metabolic Pathway; Nitrogen; Organ; Pediatric Hospitals; Pennsylvania; Philadelphia; Positioning Attribute; Principal Investigator; Pulmonology; Radiology Specialty; Request for Proposals; Research; Research Personnel; Research Project Grants; Resolution; Resource Sharing; Resources; Schedule; Signal Transduction; Spectrum Analysis; System; Technology; Time; United States National Institutes of Health; Universities; in vivo; instrument; metabolic abnormality assessment; pre-clinical; programs

DESCRIPTION (provided by applicant): This proposal requests funding to support a new, state-of-the-art, high-resolution 400- MHz magnetic resonance spectrometer and imager for studying metabolic pathways using hyperpolarized carbon and nitrogen technology. The proposed system will initially be used by thirteen NIH-funded projects and led by well-established investigators at the University of Pennsylvania, Children Hospital of Philadelphia, and the Lankenau Institute for Medical Research. At present, the proposed resource is the only feasible way to pursue further pre-clinical cell culture and animal testing of hyperpolarized carbon-13 technology. An existing NMR spectrometer at our facility is more than 25 years old, and has recently become unreliable in addition to long-standing difficulties with scheduling and availability. Incorporation of a new system with modern shielding into a dedicated hyperpolarized core will allow favorable positioning of the equipment near a DNP polarizer, and the permanent incorporation of required ancillary equipment. This will make it possible for a number of investigators to use hyperpolarized carbon-13 technology in variety of different research projects. This instrument is necessary for continued expansion of hyperpolarization studies. It will be used primarily for in vivo metabolic studies with hyperpolarized contrast agents, although several perfused organ, perfused cell mass are proposed, as is one project which makes use of DNP signal enhancement for NMR quantification of extracts. The core will be initially used by three departments at the University of Pennsylvania, two departments at the Children Hospital of Philadelphia, and the Lankenau Institute for Medical Research. We expect this number to grow substantially as the resource becomes more widely known and established. The system will be available as a shared resource to investigators at the University of Pennsylvania. Departments that will benefit include: Radiology, Pulmonary, Medicine, Biophysics and Bioengineering. This is supported by currently active collaborations between the investigators from these departments. We expect that such a system will significantly enhance investigational and collaborative research related to the emerging and powerful technology of hyperpolarized carbon-13 and its application in metabolic imaging and spectroscopy. If funded, this proposal, along with the other identified commitments, will provide the resources to make a comprehensive metabolic resource available to the investigative research programs outlined in the descriptions of individual projects. The system will be placed at and maintained by the facilities of the Department of Radiology at the University of Pennsylvania and will be managed and directed by an Internal Advisory Committee composed of the PI, co-investigators, and the principal users.

描述(由申请者提供)：这项提案要求提供资金，以支持一种新的、最先进的、高分辨率的400-MHz磁共振光谱仪和成像仪，用于研究使用超极化碳和氮技术的代谢途径。拟议的系统最初将由NIH资助的13个项目使用，并由宾夕法尼亚大学、费城儿童医院和兰克瑙医学研究所的知名研究人员领导。目前，提议的资源是进一步进行超极化碳-13技术的临床前细胞培养和动物试验的唯一可行途径。我们工厂现有的一台核磁共振波谱仪已有超过25年的历史，最近变得不可靠，此外在调度和可用性方面也存在长期的困难。将具有现代屏蔽的新系统合并到专用超极化核心中，将使设备能够有利地放置在DNP偏振器附近，并永久纳入所需的辅助设备。这将使许多研究人员有可能在各种不同的研究项目中使用超极化碳-13技术。这一仪器对于超极化研究的继续扩展是必要的。它将主要用于超极化造影剂的体内代谢研究，尽管有几个灌流器官、灌流细胞团被提出，还有一个项目利用DNP信号增强对提取物的核磁共振定量。该核心最初将由宾夕法尼亚大学的三个部门、费城儿童医院的两个部门和兰克瑙医学研究所使用。我们预计，随着资源变得更加广为人知和成熟，这一数字将大幅增长。该系统将作为共享资源提供给宾夕法尼亚大学的研究人员。受益的部门包括：放射科、肺科、内科、生物物理学和生物工程学。这得到了这些部门的调查人员目前积极合作的支持。我们预计，这样的系统将大大加强与新兴和强大的超极化碳-13技术及其在代谢成像和光谱学中的应用相关的研究和合作研究。如果得到资助，这项提案，连同其他确定的承诺，将提供资源，为个别项目说明中概述的调查性研究计划提供全面的代谢资源。该系统将放置在宾夕法尼亚大学放射学系的设施中并由其维护，并将由一个由PI、协查人员和主要用户组成的内部咨询委员会管理和指导。