MASS SPECTROMETRY CORE

质谱核心

基本信息

批准号：
7726726
负责人：
LASZLO PROKAI
金额：
$ 12.18万
依托单位：
UNIVERSITY OF NORTH TEXAS HLTH SCI CTR
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7726726
关键词：
Address Agreement Allopregnanolone Antibodies Antibody Specificity Arts Basic Science Binding Biological Assay Categories Cellular Neurobiology Chromatography Cyclotrons Cysteine Detection Electrospray Ionization Epitopes Equipment Estradiol Estrogens Estrone Evaluation Experimental Designs Fourier Transform Immunoassay Immunoblotting Ions Isotopically-Coded Affinity Tagging Laboratories Leadership Liquid Chromatography Liquid substance Longitudinal Studies Manuals Manufacturer Name Mass Spectrum Analysis Measures Methods Modification Molecular Monitor Morphologic artifacts Performance Phospho-Specific Antibodies Phosphoproteins Post-Translational Protein Processing Progesterone Protein Analysis Protein Isoforms Proteins Proteomics Protocols documentation Purpose Quality Control Radioactive Radioactivity Radioimmunoassay Range Reporting Research Personnel Sampling Sensitivity and Specificity Services Site Specialist Specificity Steroids Sulfhydryl Compounds System Techniques Testing Two-Dimensional Gel Electrophoresis Work base clinical application cross reactivity falls gel electrophoresis immunoreactivity instrument interest mass spectrometer oxidation protein expression receptor tandem mass spectrometry tool

项目摘要

The purpose of the Mass Spectrometry Core is to provide support for the projects by quantitative determination of steroids by liquid chromatography - tandem mass spectrometry (LC/MS/MS) as its major activity. Additionally, specialized services will include identification of proteins separated by gel electrophoresis, identification and quantification of Ca2+-channel/receptor isotypes and their oxidationsensitive cysteine (Cys) thiols critical to function by methods based on electrospray ionization (ESI) and LC/MS/MS. These support needs were identified by the project leaders, and the requirements/methods were discussed in details through interactions with the core director to select the most appropriate ones to be implemented as core services. Equipments made available on a shared basis are a quadrupole ion-trap mass spectrometer for steroid analysis and a linear ion-trap - Fourier-transform ion-cyclotron resonance instrument for proteomics-related work. The systems also incorporate advanced liquid chrom atographs for sample separations. Core B will provide expert leadership, specialists and technical staff to perform the core services and provide full reports on the results of quantitative steroid analyses and on protein identification, quantification to the project leaders and research personnel.

质谱核心的目的是通过定量为项目提供支持通过液相色谱 - 串联质谱法（LC/MS/MS）确定类固醇作为其主要活动。此外，专业服务将包括识别由凝胶分离的蛋白质 Ca2+通道/受体同种型的电泳，鉴定和定量及其氧化敏感通过基于电喷雾电离（ESI）和 LC/MS/MS。项目负责人确定了这些支持需求，需求/方法是通过与核心主管的互动来详细讨论，以选择最合适的主管作为核心服务实施。共享的设备是四极离子陷阱用于类固醇分析和线性离子陷阱的质谱仪 - 傅立叶 - 转变离子 - 循环共振与蛋白质组学相关的工作仪器。该系统还结合了高级液体镀铬的图表样品分离。核心B将为专家领导，专家和技术人员提供核心服务并提供有关定量类固醇分析和蛋白质识别结果的完整报告，对项目负责人和研究人员进行量化。