Formation of the Enamel-Dentin Interface

牙釉质-牙本质界面的形成

• 批准号: 7175425
• 负责人: ELIA BENIASH
• 金额: $ 2.71万
• 依托单位: ADA FORSYTH INSTITUTE, INC.
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-02-15 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7175425
• 关键词: Amelogenesis; Area; Attention; Chemicals; Data; Dental Enamel; Dentin; Dentin Formation; Development; Electrons; Elements; Enamel Formation; Failure; Goals; Hereditary Disease; Image; Implant; In Situ; In Vitro; Incisor; Indium; Knowledge; Laser Scanning Confocal Microscopy; Lead; Mechanics; Microscopic; Microtomy; Minerals; Nature; Numbers; Object Attachment; Organ; Phase; Play; Procedures; Process; Property; Proteins; Purpose; Rattus; Repair Material; Research Personnel; Resolution; Rest; Role; Sampling; Series; Site; Spectroscopy, Fourier Transform Infrared; Staging; Structure; Surface; Techniques; Testing; Tissues; Tooth structure; Wound Healing; base; biomineralization; calcium phosphate; chemical property; design; improved; in vivo; knockout animal; macromolecule; mineralization; molecular assembly/self assembly; programs; research study

DESCRIPTION (provided by applicant): The purpose of the proposed studies is to improve our understanding of the basic physico-chemical mechanisms of initial enamel mineralization and formation of the dentin-enamel interface. Enamel mineralization starts at the surface of a layer of mineralized dentin, which may play an important role in initial enamel formation. Dentin and enamel are two mineralized tissues with strikingly different mechanical and structural properties that normally perform jointly for tens of years, without failure. Such an outstanding mechanical endurance requires an extraordinarily strong bond between these two tissues. Studies of tooth related genetic disorders and knockout animals demonstrate that the correct formation of the dentin-enamel interface is essential for the proper tooth function. The problem of interface stability is also very important with respect to tissue repair, where often implant failure occurs due to a weak interface between tissues and repair materials. It is likely that interactions between dentin and enamel tissues during initial mineralization play an important role in the proper formation of this critically important interface. We hypothesize that the onset of mineralization at the boundary between enamel and dentin is a highly integrated and specialized process, essential for the proper formation of this mechanically robust interface. We also hypothesize that the mechanism of initial enamel formation at this interface is different from other stages of amelogenesis. We have identified three major factors that distinguish initial enamel formation from later stages of amelogenesis: 1) the presence of dentin mineral at the site of initial enamel formation; 2) the presence of transient mineral phases; and 3) a unique macromolecular composition at the site of initial enamel formation. Thus, we propose a comprehensive study of the formation of the dentin-enamel interface using a variety of in vivo and in vitro approaches to gain a better understanding of basic mechanisms of initial enamel formation. Specifically, we propose in Aim1: To study the interactions between dentin and enamel crystals at the site of initial enamel mineralization, with special emphasis on the epitaxial relationship between dentin and enamel crystals; in Aim 2: To study the role of transient amorphous mineral phases in initial stages of enamel formation; and in Aim 3: To study the macromolecular composition at the initial enamel mineralization site with special emphasis on protein-mineral interactions, and to elucidate the possible functions of these macromolecules and supra-molecular assemblies in initial enamel formation using a mechanistic approach, in a series of in vitro mineralization experiments. We expect that the results of this study will improve our knowledge of the basic processes involved in the formation of the dentin-enamel interface that will hopefully lead to the development of new advanced materials and/or procedures for mineralized tissue repair.

描述（由申请方提供）：拟定研究的目的是提高我们对初始釉质矿化和牙本质-釉质界面形成的基本物理化学机制的理解。釉质矿化开始于矿化牙本质层的表面，其在初始釉质形成中可能起重要作用。牙本质和牙釉质是两种矿化组织，具有显著不同的机械和结构特性，通常共同工作数十年而不会失败。如此出色的机械耐久性要求这两种组织之间具有非常强的结合力。对牙齿相关遗传疾病和基因敲除动物的研究表明，牙本质-釉质界面的正确形成对牙齿的正常功能至关重要。界面稳定性的问题对于组织修复也是非常重要的，其中通常由于组织和修复材料之间的弱界面而发生植入物失效。在初始矿化过程中，牙本质和牙釉质组织之间的相互作用可能在这一至关重要的界面的正确形成中发挥重要作用。我们假设，在釉质和牙本质之间的边界处的矿化的开始是一个高度集成和专门化的过程，对于这种机械坚固的界面的正确形成是必不可少的。我们还推测，在这个接口的初始釉质形成的机制是不同的其他阶段的釉质。我们已经确定了三个主要因素，区分初始釉质形成从釉质形成的后期阶段：1）牙本质矿物的存在在初始釉质形成的网站; 2）存在的瞬态矿物相;和3）在初始釉质形成的网站独特的大分子组成。因此，我们提出了一个全面的研究牙本质-釉质界面的形成，使用各种在体内和体外的方法，以更好地了解初始釉质形成的基本机制。具体而言，我们在目标1中提出：研究初始釉质矿化部位牙本质和釉质晶体之间的相互作用，特别强调牙本质和釉质晶体之间的外延关系;在目标2中提出：研究瞬时无定形矿物相在釉质形成初始阶段的作用;在目标3中提出：为了研究初始釉质矿化部位的大分子组成，特别强调蛋白质-矿物质相互作用，并使用机械方法阐明这些大分子和超分子组装体在初始釉质形成中的可能功能，在一系列体外矿化实验中。我们期望本研究的结果将提高我们对牙本质-釉质界面形成中所涉及的基本过程的认识，这将有望导致开发用于矿化组织修复的新的先进材料和/或程序。