Formation of the Enamel-Dentin Interface

牙釉质-牙本质界面的形成

基本信息

批准号：
7782760
负责人：
ELIA BENIASH
金额：
$ 26.93万
依托单位：
UNIVERSITY OF PITTSBURGH AT PITTSBURGH
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-02-15 至 2013-01-31
项目状态：
已结题

项目摘要

The purpose of the proposed studies is to improve our understanding of the basic physico-chemical mechanisms of initial enamel mineralization and formation of the dentin-enamel interface. Enamel mineralization starts at the surface of a layer of mineralized dentin, which may play an important role in initial enamel formation. Dentin and enamel are two mineralized tissues with strikingly different mechanical and structural properties that normally perform jointly for tens of years, without failure. Such an outstanding mechanical endurance requires an extraordinarily strong bond between these two tissues. Studies of tooth- related genetic disorders and knockout animals demonstrate that the correct formation of the dentin-enamel interface is essential for the proper tooth function. The problem of interface stability is also very important with respect to tissue repair, where often implant failure occurs due to a weak interface between tissues and repair materials. It is likely that interactions between dentin and enamel tissues during initial mineralization play an important role in the proper formation of this critically important interface. We hypothesize that the onset of mineralization at the boundary between enamel and dentin is a highly integrated and specialized process, essential for the proper formation of this mechanically robust interface. Wealso hypothesize that the mechanism of initial enamel formation at this interface is different from other stages of amelogenesis. We have identified three major factors that distinguish initial enamel formation from later stages of amelogenesis: 1) the presence of dentin mineral at the site of initial enamel formation; 2) the presence of transient mineral phases; and 3) a unique macromolecular composition at the site of initial enamel formation. Thus, we propose a comprehensive study of the formation of the dentin-enamel interface using a variety of in vivo and in vitro approaches to gain a better understanding of basic mechanisms of initial enamel formation. Specifically, we propose in Aim1: To study the interactions between dentin and enamel crystals at the site of initial enamel mineralization, with special emphasis on the epitaxial relationship between dentin and enamel crystals; in Aim 2: To study the role of transient amorphous mineral phases in initial stages of enamel formation; and in Aim 3: To study the macromolecular composition at the initial enamel mineralization site with special emphasis on protein-mineral interactions, and to elucidate the possible functions of these macromolecules and supra-molecular assemblies in initial enamel formation using a mechanistic approach, in a series of in vitro mineralization experiments. We expect that the results of this study will improve our knowledge of the basic processes involved in the formation of the dentin-enamel interface that will hopefully lead to the development of new advanced materials and/or procedures for mineralized tissue repair.

拟议研究的目的是提高我们对基本物理化学化学的理解初始搪瓷矿化的机理和牙本质 - 烯象界面的形成。搪瓷矿化始于一层矿化牙本质的表面，这可能在初始角色中起重要作用搪瓷形成。牙本质和搪瓷是两个矿化组织，具有明显不同的机械和通常共同执行数十年的结构特性而不会失败。如此出色机械耐力需要这两种组织之间非常牢固。牙齿研究相关的遗传疾病和淘汰动物表明，牙本质 - 永胺的正确形成界面对于适当的牙齿功能至关重要。接口稳定的问题也非常重要关于组织修复，在组织和维修材料。初始矿化过程中牙本质和搪瓷组织之间的相互作用可能很可能在此至关重要的界面的正确形成中起重要作用。我们假设搪瓷和牙本质之间边界处的矿化发作是高度整合的，专业过程，对于正确形成此机械稳健的界面至关重要。韦尔索假设在该界面处初始搪瓷形成的机理与其他不同杂货发生的阶段。我们已经确定了区分初始搪瓷形成的三个主要因素从未来的阶段开始：1）在初始搪瓷形成部位存在牙本质矿物质； 2）瞬时矿物相的存在； 3）在初始位置的独特大分子组成搪瓷形成。因此，我们提出了牙本质 - 内聚物界面形成的全面研究使用各种体内和体外方法，以更好地了解初始搪瓷形成。具体而言，我们在AIM1中提出：研究牙本质与初始搪瓷矿化部位的搪瓷晶体，特别强调外延关系在牙本质和搪瓷晶体之间；在AIM 2中：研究瞬态无定形矿物相中的作用搪瓷形成的初始阶段；并在AIM 3中：研究最初的大分子成分搪瓷矿化部位特别强调蛋白质矿物质相互作用，并阐明在初始搪瓷形成中这些大分子和上分子组件的可能功能在一系列体外矿化实验中，使用机械方法。我们期望结果这项研究将提高我们对形成涉及的基本过程的了解牙本质 - 烯象界面将有望导致开发新的高级材料和/或矿化组织修复的程序。