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Three Dimensional Holography for Parallel Multi-target Optogenetic Circuit Manipulation

用于并行多目标光遗传学电路操纵的三维全息术

基本信息

批准号：
8826957
负责人：
Valentina Emiliani
金额：
$ 42.77万
依托单位：
PIERRE AND MARIE CURIE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-09-30 至 2017-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8826957
关键词：
Address Algorithms Behavior Brain Calcium Cells Cognition Collaborations Communication Communities Development Dimensions Disease Engineering Esthesia Financial compensation Fishes Functional disorder Generations Glutamates Goals Heating Heterogeneity Holography Image In Vitro Industry Collaborators Investigation Kinetics Laboratories Language Larva Lasers Lateral Light Location Methods Microelectrodes Morphologic artifacts Motor Movement Neurons Neurosciences Opsin Optics Output Pattern Perception Photons Physiology Positioning Attribute Preparation Property Proteins Resolution Retina Shapes Site Spottings Stimulus System Techniques Technology Testing Thick Time Tissues Validation Zebrafish absorption brain tissue cognitive function computer generated density design flexibility improved in vivo innovation insight laser tweezer lens microbial nervous system disorder neural circuit optogenetics photoactivation prototype public health relevance relating to nervous system tool two-photon

项目摘要

DESCRIPTION (provided by applicant): Understanding communication between neurons, who is talking to whom, and what language they are speaking, is essential for discovering how brain circuits underlie brain function and dysfunction. Over the past decades, Neuroscience has made exponential progress toward recording and imaging communication between neurons. In addition, geneticists have recently developed the capability to manipulate neurons with light through the expression of light-activated microbial proteins called "opsins." Now, neuroscientists can drive neural circuits in order to determine how they give rise to sensation, perception, and cognitive function. In order to take full advantage of "optogenetic" tools, we are developing holographic methods to deliver patterned light into brain tissue, to enable simultaneous activation of multiple neurons, independently controlling the strength and timing of light targeted to each cell. Here, we propose to: (1) characterize newly developed opsins to determine which are best suited for holographic activation techniques; (2) implement holographic light patterns in three-dimensions; and, (3) distribute and iteratively optimize the 3D holography system in collaboration with Neuroscientists studying circuits in optically and physiologically diverse neura systems. The end goal is to develop a robust system, capable of manipulating neurons in patterns that mimic naturally occurring activity. Insights gained through this collaborative optimization will be used to inform the design of the commercial prototype developed by our industry collaborator Intelligent Imaging Innovations, Inc. (Denver, CO). The system can thus be widely distributed for neural circuit investigation, both in-vitro and in-vivo, to discover how neual communication gives rise to sensation, perception, cognition, and behavior. Such insights will improve our ability to identify effective targets and methods for treating neurological diseases and disorders.