ShEEP Request For Gas Chromatograph Mass Spectrometer

ShEEP 请求气相色谱质谱仪

• 批准号: 9795013
• 负责人: ANDREW J MORRIS
• 金额: --
• 依托单位: VA MEDICAL CENTER - LEXINGTON, KY
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-01-01 至 2019-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9795013
• 关键词: 15 year old; Address; Agreement; Air; Area; Blood coagulation; Brain; Brain Injuries; Businesses; Cardiovascular Diseases; Clinical; Computer Workstations; Computer software; Contract Services; Cost Measures; Data; Data Analyses; Development; Diet; Digestive System Disorders; Doctor of Philosophy; Electrons; Ensure; Fee-for-Service Plans; Focus Groups; Funding; Gas Chromatography; Gases; Health; Healthcare Systems; Heart Diseases; Human; Human Resources; Individual; Injections; Injury; Institutional Review Boards; Investments; Kentucky; Knowledge; Libraries; Maintenance; Mass Spectrum Analysis; Measures; Medical Research; Medical center; Medicine; Methods; Monitor; Muscle Weakness; Neurodegenerative Disorders; Oils; Patients; Peer Review; Play; Process; Productivity; Publications; Pump; Recovery; Regulation; Research; Research Personnel; Research Project Grants; Research Support; Retinal; Role; Sampling; Scientist; Sepsis; Services; Sheep; Signal Transduction; Solvents; Source; Speed; System; Technical Expertise; Training; Traumatic Brain Injury; Ulcerative Colitis; Universities; Validation; Veterans; Work; assay development; beneficiary; college; cost; data acquisition; detector; environmental chemical; equipment acquisition; experience; flexibility; human disease; improved; indexing; instrument; instrumentation; ionization; lipid metabolism; malignant breast neoplasm; mass spectrometer; member; operation; programs; research and development

The purpose of this application is to request funds for acquisition of an integrated gas chromatography mass spectrometer system (GC MS) with an electron capture detector and a workstation computer with dedicated software for instrument control, data acquisition and analysis. The instrument system requested comprises an Agilent Intuvio 9000 gas chromatograph with an Agilent 7693A auto injector an air cooled multimode inlet (enables split/splitless and large volume injections) and an Agilent micro electron capture detector with a splitter chip. The system is interfaced with an Agilent 5977B single quadrupole mass spectrometer with a High Efficiency Electron Impact ionization source and an oil free scroll roughing pump. The system includes a Workstation Computer with Agilent Mass Hunter software for instrument control and data analysis with the 2017 NIST library of 307,000 spectra for 267,000 compounds and retention indices for 87,000 compounds. This instrument system will be primarily used by 4 VA funded researchers (Major Users) for research in areas that include lipid metabolism and signaling in cardiovascular disease, the effects of diet and environmental chemicals on human health, regulation of blood clotting, treatment of muscle weakness in hospitalized patients, management and treatment of patients with ulcerative colitis, breast cancer and blast induced brain and retinal injuries. These are hematic areas of notable strength at the Lexington VA Medical Center and represent areas of medical research that are particularly relevant to Veterans. The instrument will be overseen and operated by highly experienced analytical chemists who will work together with the Major Users to ensure the requested instrument system is used efficiently to generate high quality reliable data to support their VA funded research projects. Administrative mechanisms are in place to control and monitor access to the instrument system. A business plan will be enacted to ensure sustainability of the instrument system so that the investment requested from this funding program will be repaid with a high level of research productivity.

本申请的目的是申请资金，用于购买集成的气相色谱质谱仪 光谱仪系统（GC MS），配有电子捕获检测器和工作站计算机， 仪器控制、数据采集和分析软件。所要求的仪器系统包括 Agilent Intuvio 9000气相色谱仪，配备Agilent 7693 A自动进样器和空气冷却多模式入口 （能够分流/不分流和大体积进样）和Agilent微电子捕获检测器， 分离器芯片该系统与具有高灵敏度的Agilent 5977 B单四极杆质谱仪连接。 高效率的电子轰击电离源和无油涡旋式低真空泵。该系统包括 配备Agilent Mass Hunter软件的工作站计算机，用于仪器控制和数据分析， 2017年NIST数据库包含267，000种化合物的307，000个光谱和87，000种化合物的保留指数。 该仪器系统将主要由4名VA资助的研究人员（主要用户）用于以下领域的研究 包括心血管疾病中的脂质代谢和信号传导，饮食和环境的影响， 化学品对人体健康，调节血液凝固，治疗肌肉无力住院 溃疡性结肠炎、乳腺癌和脑爆震患者的管理和治疗 和视网膜损伤这些是列克星敦退伍军人医疗中心的显著优势的出血区域， 代表与退伍军人特别相关的医学研究领域。该仪器将由 由经验丰富的分析化学家操作，他们将与主要用户合作，以确保 要求的仪器系统被有效地用于生成高质量的可靠数据，以支持他们的VA 资助的研究项目。建立了管理机制，以控制和监测对 仪器系统将颁布一项业务计划，以确保仪器系统的可持续性， 这项资助计划所要求的投资将以高水平的研究生产力来偿还。