ShEEP Request for Keyence BZ-X800E All-in-One Automated Imaging System

该共享设备评估计划(SHEEP)提案的目标是要求Keyence BZX800E一体式自动成像系统将为研究人员提供一种能够： 共焦式多层叠成像和厚组织切片的实时聚焦；快速、自动、高分辨率 复合图像的放大图像采集和拼接；序列的三维仿形 组织切片以高分辨率成像。控制软件简单直观。因此，用最少的 训练新手用户可以扫描整个器官的一系列组织切片，生成高分辨率 每个组织切片的组合，并将所有组合合并到单个三维渲染中 整个组织。巴尔的摩退伍军人医疗中心(BVAMC)提供的传统显微镜不具备 这些能力。 退伍军人管理局的项目涵盖了如下所述的一系列专业。每个人都将受益于Keyence BZX800E 一体式自动成像系统。BZX800E显微镜通过以下方式优化成像过程 设计一种全自动、易于使用的系统，以捕获高分辨率图像并允许研究人员 以更快地达到预期效果。这些应用对VA生物医学实验室非常有用 研究与开发服务(BLR&D)、临床科学研究与开发(CSR&D)以及 康复研究与开发(RR&D)资助了调查人员。Tibor Kristian博士将担任 本提案的项目主任(PD)/首席调查员(PI)。克里斯蒂安博士的研究重点是 了解导致线粒体并最终导致细胞生物能衰竭的损伤机制 中风患者。这种成像系统对于细胞内细胞器的成像和定量是必不可少的。 卒中所致缺血性脑损伤后的损害。胡炳仁，医学博士，博士正在检验一种新的假说 脑缺血-再灌流导致一系列事件导致失活；以及研究新奇 脑缺血再灌注损伤的机制。AIMS 1和AIMS 2中将使用许多抗体 而BZX800显微镜是细胞内成像和定量所必需的 IR脑损伤后细胞器损伤。医学博士Bogdan Stoica将使用这一新系统进行研究 创伤性脑损伤(TBI)。斯托伊卡博士的项目需要对慢性神经炎进行重要的成像 标志物和二次破坏性标志物。塔帕斯·马卡尔博士的S项目研究7，8- 二羟基黄酮(7，8-DHF)；它具有强大的脑源性神经营养因子(BDNF)激动剂活性和 将在他的具体目标2和3中使用成像系统。Makar博士将确定DHF治疗 调节脑脊髓炎(EAE)小鼠模型中脑和脊髓的炎症和免疫 多发性硬化症，使用单抗。莱昂纳多·托内利博士的S项目研究了 部署和暴露在创伤事件中，使士兵面临比普通公众更大的风险 发展成心理障碍。Tonelli博士的分析将包括邻近的 归巢标记、趋化因子受体、小胶质细胞和巨噬细胞激活和/或 与众不同-所有这些都可以通过Keyence BZ-X800查看。谢国锋，医学博士，研究结肠癌 他的长期目标是通过靶向关键信号来开发创新、有效和安全的治疗方法 结肠癌进展的转导分子。这台显微镜将允许谢博士检查 免疫荧光抗MMP7抗体对人卵巢癌细胞MMP7表达的影响 人结肠癌细胞和手术标本。受资助的项目构成了翻译研究的基础。 这将改善退伍军人的治疗-潜在地产生新的治疗方法。

The objective of this Shared Equipment Evaluation Program (ShEEP) proposal is to request Keyence BZX800E All-in-One Automated Imaging System which will offer researchers an instrument that is capable of: confocal-like multi-stack imaging and real-time focusing of thick tissue sections; rapid automatic high- magnification image acquisition and stitching of composite images; and three-dimensional profiling of serial tissue sections imaged at high resolution. The control software is simple and intuitive. Thus, with minimal training a novice user can scan a series of tissue sections from an entire organ, generate high-resolution composites of each tissue section, and merge all composites into a single three-dimensional rendering of the entire tissue. Traditional microscopes available at the Baltimore VA Medical Center (BVAMC) do not possess these capabilities. The VA projects cover an array of specialties as described below. Each will benefit from Keyence BZX800E All-in- One Automated Imaging System. The BZX800E Microscope optimizes the imaging process by designing a fully automated, easy-to-use system that captures high-resolution images and allows researchers to achieve their desired results faster. These applications are of great use for VA Biomedical Laboratory Research and Development Service (BLR&D), Clinical Science Research and Development (CSR&D), and Rehabilitation Research and Development (RR&D) funded investigators. Tibor Kristian, Ph.D. will serve as the Project Director (PD)/ Principal Investigator (PI) for this proposal. Dr. Kristian’s study focuses on understanding injury mechanisms that lead to mitochondrial and ultimately cellular bioenergetic failure in stroke victims. This imaging system is essential for imaging and quantification of intracellular organelles damage following stroke- induced ischemic brain injury. Bingren Hu, M.D., Ph.D. is testing a novel hypothesis that brain ischemia- reperfusion leads to a cascade of events resulting in inactivation; as well as study novel mechanisms underlying brain ischemia-reperfusion (IR) injury. Many antibodies will be used in Aims 1 and 2 of Dr. Hu’s studies; and the BZX800 microscope is necessary for imaging and quantification of intracellular organelles damage following IR brain injury. Bogdan Stoica, M.D. will use this new system to investigate traumatic brain injury (TBI). Dr. Stoica’s project requires significant imaging of chronic neuroinflammation markers and secondary damaging markers. Tapas Makar, Ph.D.’s project studies the effects of 7, 8- dihydroxyflavone (7,8- DHF); which has potent brain derived neurotrophic factor (BDNF) agonist activity and will use the imaging system in his specific aims 2 and 3. Dr. Makar will determine whether DHF treatment modulates inflammation and immunity in the brain and spinal cord in encephalomyelitis (EAE), a murine model of Multiple sclerosis, using monoclonal antibodies. Leonardo Tonelli, Ph.D.’s project studies the stress of deployment and exposure to traumatic events that puts soldiers at a greater risk than the general public in developing psychological disorders. Dr. Tonelli’s analysis will consist of immunohistochemistry in adjacent sections for: homing markers, chemokine receptors, microglia and macrophage activation and/or differentiation- all of which can be viewed with the Keyence BZ-X800. Guofeng Xie, M.D. studies colon cancer and his long-term goal is to develop innovative, effective, and safe therapies by targeting pivotal signal transduction molecules underlying colon cancer progression. The microscope will allow Dr. Xie to examine the expression patterns of matrix metalloproteinase 7 (MMP7) using immunofluorescent anti-MMP7 antibody in human colon cancer cells and surgical specimens. The funded projects form the basis for translational studies that will improve treatment in Veterans- potentially yielding new therapeutics.