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T cell compartmentalization and antiviral response

T 细胞区室化和抗病毒反应

基本信息

批准号：
9127649
负责人：
Arash Grakoui
金额：
$ 64.13万
依托单位：
EMORY UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-03-01 至 2021-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9127649
关键词：
Acute Address Affect Affinity Anatomy Antigens Antiviral Agents Antiviral Response Apoptosis Autoimmunity B-Lymphocytes Binding Biochemical Biochemical Reaction Biological Blood CD3 Antigens CD4 Positive T Lymphocytes CD8B1 gene Calcium Signaling Cells Communication Complex DNA Probes Data Dendritic Cells Development Discrimination Effector Cell Environmental Risk Factor Epitopes Equilibrium Escape Mutant Exhibits Functional disorder Future Genes Helper-Inducer T-Lymphocyte Histocompatibility Immune Immune response Immunoglobulin Somatic Hypermutation In Situ Infection Inflammatory Infusion procedures Invaded Lead Ligand Binding Liver Fibrosis Liver diseases Location Lymphocytic choriomeningitis virus Malpighian corpuscles Measurement Measures Memory Methods Molecular Monoclonal Antibodies Mus Natural Killer Cells Outcome Peptide/MHC Complex Peptides Peripheral Phase Phosphoric Monoester Hydrolases Phosphotransferases Population Post-Translational Protein Processing Proliferating Regulation Role Signal Transduction Site Sorting - Cell Movement Splenic Red Pulp Systems Biology T cell differentiation T cell response T memory cell T-Cell Receptor T-Lymphocyte Technology Time Tissues Transforming Growth Factor beta Transgenic Organisms Viral Virus Virus Diseases alpha-beta T-Cell Receptor arm base cell killing chronic liver disease cytokine cytotoxicity differential expression glycosylation immunogenic in vivo insight interest intrahepatic macrophage novel pathogen precursor cell prevent public health relevance release of sequestered calcium ion into cytoplasm response targeted treatment two-dimensional

项目摘要

DESCRIPTION (provided by applicant): The T cell receptor (TCR) recognizes antigen in the context of a major histocompatibility (MHC) molecule, which is critical for determining T cell fate during thymic development as well as peripheral activation, differentiation and memory development. As a mechanosensor, the TCR complex interprets the information embedded in the peptide-MHC and transduces it from the ligand-binding interface across the TCR αβ chains to the associated CD3 subunits into biochemical signals in the form of binding/unbinding of kinase/ phosphatase molecules and their enzymatic reactions. During an acute resolving infection such as LCMV Arm in mice, naïve CD8+ T cells will proliferate and expand during the first week of infection. This expansion is antigen-specific and dependent on help from panoply of co-stimulatory and inflammatory cytokines. If successful and the virus is cleared, the majority of these cells will die by apoptosis during the contraction phase that lasts typically between 8-30 days post infection. A small portion of these effector cells will then differentiate to memory T cells where they can be poised to respond to a recall antigen quicker and with more vigor than during the primary response. Since tissue-resident immune cells are known to be phenotypically and functionally different from cells circulating in the blood, discrimination of cells that particpate in local immune responses and those that are circulating in the vasculature is of great interest. In this study, we hypothesize that the anatomic compartmentalization of anti-viral CD8+ T cells affect TCR-pMHC interaction and functional differentiation of T cells during the immune contraction phase. This hypothesis is based on our recent analysis of effective 2D TCR affinity, measured in situ by highly sensitive two-dimensional (2D) methods. Anti-viral CD8+ T cell responses reach a peak between 7-8 days post infection with subsequent elimination of viral titers by day 8. Surprisingly, the 2D TCR affinity of genetically identical P14 TCR on T cells in the splenic red pulp was increased ~5 fold as compared to that of the WP T cells on days 9 and 11 after the cessation of anti-viral immune responses. These data suggest that differentially localized anti-viral CD8+ T cells receive disparate signals from distinct microenvironments, thus potentially affecting their function and differentiation. In this proposal, we aim to understand ho anatomic localization of anti-viral CD8+ T cells regulates TCR-pMHC interaction and potentially influence the fate of antigen-specific T cells. Immune cells continuously circulate to patrol the body searching for invading pathogens. Upon infection, many immune cells are locally accumulated into the site of infection to more efficiently clear the pathogen and prevent the spread of infection. The specific location of these immune cells, whether they are resident in the white pulp or red pulp may have important implication for how strongly they react to the pathogen, preferentially expand, die or become long lasting memory cells poised to respond to a recall antigen quicker and with more vigor than during the primary response. These differentially localized immune cells may receive disparate signals from distinct microenvironments, thus potentially affecting their function and differentiation.