TRD3: Percutaneous and Interstitial Imaging

TRD3：经皮和间质成像

• 批准号: 9275998
• 负责人: Martin Villiger
• 金额: $ 35.69万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9275998
• 关键词: Ablation; Acute; Address; Adopted; Algorithms; Anatomy; Animal Experimentation; Animal Model; Animals; Aorta; Arrhythmia; Arterial Fatty Streak; Arteries; Astigmatism; Atherosclerosis; Biological; Birefringence; Blood; Blood Vessels; Blood flow; Caliber; Cardiac; Cardiac Catheterization Procedures; Carotid Arteries; Carotid Artery Ulcerating Plaque; Catheters; Chronic; Clinic; Clinical; Clinical Management; Coagulation Process; Collagen; Collagen Fiber; Complex; Contrast Media; Coronary; Coronary artery; Cystic Fibrosis; Deep Vein Thrombosis; Detection; Development; Devices; Diagnosis; Diagnostic; Diagnostic Imaging; Esophageal; Ferrets; Fiber; Fiber Optics; Functional Imaging; Goals; Heart Atrium; Human; Image; Inflammatory Response; Injectable; Injection of therapeutic agent; Intervention; Light; Lighting; Location; Lung; Lymphatic; Maps; Measurement; Measures; Mechanics; Methods; Microscope; Microscopic; Modeling; Monitor; Morphology; Mus; Myocardial Ischemia; Neoplasms; Optical Coherence Tomography; Optical Rotation; Optics; Organ; Patients; Pattern; Performance; Positioning Attribute; Property; Protocols documentation; Rattus; Records; Recurrence; Research; Resolution; Rotation; Scanning; Side; Signal Transduction; System; Techniques; Thrombus; Time; Tissues; Translations; Vascular System; Vascular remodeling; Vasodilation; Veins; Vena caval structure; abdominal aorta; animal imaging; atherogenesis; base; clinical imaging; endothelial dysfunction; flexibility; high resolution imaging; hypodermic needle; image guided; improved; innovation; instrument; interstitial; minimally invasive; mouse model; percutaneous coronary intervention; pre-clinical; preclinical study; reconstruction; response; signal processing; tool; vector

Project Summary TRD 3 The overall goal of TRD 3 is to enable advanced OCT imaging, with high resolution and functional and dynamic contrast, inside the living body, through narrow diameter, rotational probes. Instruments and algorithms will be developed that overcome fundamental barriers, intrinsic to flexible, rotational fiber-optic probes, that have previously compromised image quality and prohibited the integration of functional and dynamic contrast. Specific Aim 1 addresses the need for functional imaging of blood flow in the coronary arteries. In catheter- based OCT a side-looking fiber-optic probe performs a helical scan pattern and records the reflectivity profile as a function of depth at each scan location. For normal imaging of the arteries, the blood is displaced by injecting a clear contrast agent. Recording, instead, time sequences without injection produces a rapidly fluctuating signal related to the blood flow. Based on accurate modeling of this signal, a reconstruction algorithm to extract vectorial blood flow profiles will be developed. Eliciting the overall flow rate offers a key parameter for the assessment of atherosclerotic lesions in human coronary arteries. Flow is also a critical factor in endothelial dysfunction and atherogenesis. The ability to map turbulence and detailed flow profiles offers a new avenue for the study of preclinical mouse models of atherosclerosis. Specific Aim 2 explores the polarization properties of biological tissue. Polarization sensitive detection measures the polarization state of the backscattered light. Combined with two orthogonal polarization states for illumination, this provides a complete characterization of the polarization properties of the tissue. However, the spinning catheter and the superimposed tissue impact and bias the polarization states at each depth. A reconstruction strategy to extract the depth-resolved polarization properties, including birefringence, optic axis and depolarization will be developed. Polarization contrast will help in differentiating between acute and chronic thrombus, which is an unresolved challenge in the clinical management of deep vein thrombosis, and in identifying different plaque types in atherosclerosis. Specific Aim 3 responds to the need for catheter-based imaging in preclinical small animal research. Narrow diameter rotational OCT probes, small enough to access the vascular system in small animals, or image interstitial tissue accessed through the bore of a hypodermic needle will be developed. The miniature probes will compensate the astigmatism originating from the transparent narrow diameter sheath that is protecting the spinning probe, and achieve a high quality and tight focus. Combined with a stable scanning mechanism this will enable high resolution imaging of the minute anatomy in the organs of small animals. Combined, these efforts will improve the diagnostic capability of OCT by providing additional contrast and functional parameters and expand the application of OCT to tissues and organs previously out of its reach.

项目摘要 TRD 3 TRD 3的总体目标是实现先进的OCT成像，具有高分辨率和功能， 动态对比，在活体内，通过窄直径，旋转探头。文书和 将开发克服柔性旋转光纤固有的基本障碍的算法 探头，以前已经损害了图像质量，并禁止功能和 动态对比 特定目标1解决了冠状动脉中血流功能成像的需求。在导管中- 基于OCT，侧视光纤探头执行螺旋扫描模式并记录反射率轮廓 作为每个扫描位置处的深度的函数。对于动脉的正常成像，血液被 注射透明的造影剂相反，记录时间序列而不注射产生快速的 与血流有关的波动信号。基于对该信号的精确建模， 将开发用于提取矢量血流轮廓的算法。得出总流速提供了一个关键 用于评估人冠状动脉中动脉粥样硬化病变的参数。流动也是一个关键 内皮功能障碍和动脉粥样硬化形成的因素。能够绘制湍流和详细的流动剖面图 为动脉粥样硬化的临床前小鼠模型的研究提供了新的途径。 具体目标2探索生物组织的偏振特性。偏振灵敏检测 测量后向散射光的偏振状态。结合两个正交偏振态， 通过使用照明，这提供了组织的偏振特性的完整表征。但 旋转的导管和叠加的组织影响并偏置每个深度处的偏振状态。一 重建策略，以提取深度分辨偏振特性，包括双折射，光轴 并且将产生去极化。偏振对比度将有助于区分急性和 慢性血栓，这是深静脉血栓临床管理中尚未解决的挑战，以及 在识别动脉粥样硬化中不同类型的斑块方面。 Specific Aim 3响应了临床前小动物研究中对基于导管的成像的需求。窄 直径旋转OCT探头，小到足以进入小动物的血管系统，或成像 通过皮下注射针的孔进入的间质组织将发育。微型探测器 将补偿源自保护所述透镜的透明窄直径护套的像散， 旋转探头，实现高质量和紧密聚焦。结合稳定的扫描机制， 将使小动物器官中微小解剖结构的高分辨率成像成为可能。 结合起来，这些努力将通过提供额外的对比度来提高OCT的诊断能力， 功能参数，并将OCT的应用扩展到以前无法达到的组织和器官。