PROJECT 4: MOLECULAR AND CELLULAR MECHANISMS FOR THERMAL ROBUSTNESS AND MULTISEN

项目 4：热鲁棒性和 Multisen 的分子和细胞机制

• 批准号: 9274837
• 负责人: Yun Zhang
• 金额: $ 51.93万
• 依托单位: BRANDEIS UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2019-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9274837
• 关键词: Adult; Affect; Animals; Behavior; Behavior Control; Biological Models; Biology; Body Temperature; Caenorhabditis elegans; Cations; Critical Pathways; Data; Detection; Disease; Disease Vectors; Drosophila genus; Esthesia; Family; Financial compensation; G-substrate; GTP-Binding Proteins; Genetic; Head; Health; Homeostasis; Human; Inflammation; Insect Control; Insect Vectors; Lead; Light; Malaria; Mammals; Mediating; Molecular; Movement; Nature; Neurons; Oocytes; Pain; Pathway interactions; Peripheral; Physiologic Thermoregulation; Physiological; Physiology; Protein Isoforms; Proteins; Rana; Regulation; Signal Pathway; Signal Transduction; Site; TRPA1 Channel; Temperature; Temperature Sense; Testing; The Sun; West Nile virus; Work; experimental study; fly; human disease; insect disease; insight; malaria transmission; novel; preference; receptor; response; sensor

Temperature strongly affects physiology and behavior, and thermosensation is relevant to body temperature homeostasis and pain in humans, host seeking by insect disease vectors, and movement in all animals. Using D. melanogaster as a model system, we propose to define a novel molecular pathway for thermal sensing involving a previously unappreciated class of potential thermal sensing protein. This work will thus lead to the definition of a new molecular pathway for thermal sensing of potentially broad relevance for both basic biology and human health. In preliminary experiments, we have identified a critical regulator of themnosensory behavior. Furthermore, we have found that this protein is capable of conferring thermal sensitivity upon a neuron when ectopically expressed. Together our preliminary data suggest this protein may function as a previously unappreciated type of thermal sensor. We propose to extend these studies in three aims. 1) We will investigate the site of action and molecular nature ofthe putative thermal sensor in controlling thermotactic behavior. 2) We will examine how this putative thermal sensor affects temperature sensing by thermosensory neurons. 3) We will examine the molecular mechanisms by which the thermal sensor detects temperature by examining its potential signaling mechanism and the signaling pathways with which it may interact to transduce temperature infonnation. Together, these studies will provide fundamental new insights into the molecular mechanisms of thermal sensation. RELEVANCE (See instmctions): This proposal investigates the molecular mechanisms of thermal sensation. Thermosensation is critical for animal sun/ival and physiology. In humans, thermosensation is critical for pain, inflammation and tx)dy temperature regulation. Thus, thermosensory mechanisms are of biomedical relevance. In addition, themosensation is important for host-seeking by insect vectors of human diseases like malaria and West Nile. Thus the study of thermosensation is relevant to the control of insect-borne human disease.

温度强烈影响生理和行为，热感觉与体温有关