Molecularly Imprinted Polymer-Carbon Nanotube Sensors for the Detection of Magnesium

用于检测镁的分子印迹聚合物碳纳米管传感器

• 批准号: 9815919
• 负责人: Nathan Romero
• 金额: $ 4.91万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-30 至 2020-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9815919
• 关键词: Affinity; Alkenes; Benchmarking; Binding; Binding Sites; Biochemical Process; Biological; Biological Models; Biological Testing; Carbon Nanotubes; Cations; Cells; Characteristics; Chelating Agents; Complex; Cyclization; Detection; Devices; Diabetes Mellitus; Diagnosis; Diagnostic tests; Disease; Elements; Environment; Event; Excision; Extravasation; Goals; Health; Human; Hybrids; Hypertension; Immunodeficiency and Cancer; Immunologic Deficiency Syndromes; Ions; Ligands; Link; Magnesium; Measurement; Measures; Modeling; Modification; Modulus; Molecular; Molecular Target; Monitor; Morphology; Organism; Physiological; Plasma; Polymers; Preparation; Property; Regulation; Reporting; Research; Resistance; Role; Sampling; Serum; Signal Transduction; Specificity; Spectroscopy, Fourier Transform Infrared; Structure; Surface; System; Techniques; Technology; Testing; Titrations; Work; aqueous; base; biological systems; chelation; chemical property; design; experimental study; imprint; improved; molecular recognition; monomer; point of care; point-of-care diagnostics; polymerization; preference; pyridine; sensor; success; tool

PROJECT SUMMARY/ABSTRACT Magnesium (Mg2+) is an essential element found in all cells and is intimately involved in a myriad of biochemical processes and physiological functions. Improper regulation of Mg2+ in humans has been linked to diseases such as diabetes, hypertension, and immunodeficiency. Therefore, monitoring Mg2+ levels could aid in diagnosis and treatment of these disease states; however, current technologies for measurement of Mg2+ are not amenable to rapid, point-of-care analysis, and are limited by a need for extensive sample preparation or a lack of selectivity for Mg2+ over other cations. This proposal describes a hybrid carbon nanotube-molecularly imprinted polymer (CNT-MIP) chemiresistive sensor designed to selectively detect Mg2+. The proposed system consists of a CNT network modified with a functional enediyne that triggers the formation of a diradical upon binding Mg2+. In the presence of alkene- containing monomers, the diradical initiates a polymerization that propagates around the Mg2+-complexed template molecule. Removal of the Mg2+ template affords a polymer-coated CNT network with binding cavities that are expected to be highly selective for the recognition of Mg2+, and the binding event will be detected using conductometric measurements. Because the functional enediyne acts as both an initiator and an Mg2+-binding group, precise spatial control of the binding sites near the CNT network can be achieved while maintaining a direct connection from the binding site to the CNT. The MIP portion of the sensor is expected to provide the CNT network with structural robustness and resistance to deleterious bulk current leakage, which is normally problematic in aqueous CNT-based sensors.

项目摘要/摘要 镁（Mg2+）是所有细胞中发现的重要元素，与无数 生化过程和生理功能。人类中MG2+的不当调节已与 糖尿病，高血压和免疫缺陷等疾病。因此，监视MG2+水平可以帮助 在这些疾病状态的诊断和治疗方面；但是，当前测量MG2+的技术 不适合快速的即时分析，并且受到大量样品准备的需求的限制 或对其他阳离子缺乏对MG2+的选择性。 该提案描述了一种混合碳纳米管 - 分子印刷聚合物（CNT-MIP）化学物质 传感器旨在选择性检测MG2+。提出的系统由通过A修改的CNT网络组成 功能性启动，在结合Mg2+时会触发脏物的形成。在烯烃存在的情况下 包含单体，Diradical启动了聚合，该聚合围绕MG2+复合传播 模板分子。去除MG2+模板可提供具有结合腔的聚合物涂层网络 预计将对MG2+的识别具有很高的选择性，并且将使用绑定事件检测 导电测量。因为功能性EneDiyne既充当启动器又是MG2+结合 小组，可以在维持CNT网络附近的结合位点的精确空间控制。 从绑定位点与CNT的直接连接。传感器的MIP部分有望提供 CNT网络具有结构性鲁棒性和抵抗有害的大量电流泄漏，通常是 在基于CNT水性的传感器中有问题。