Molecularly Imprinted Polymer-Carbon Nanotube Sensors for the Detection of Magnesium

用于检测镁的分子印迹聚合物碳纳米管传感器

• 批准号: 9815919
• 负责人: Nathan Romero
• 金额: $ 4.91万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-30 至 2020-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9815919
• 关键词: Affinity; Alkenes; Benchmarking; Binding; Binding Sites; Biochemical Process; Biological; Biological Models; Biological Testing; Carbon Nanotubes; Cations; Cells; Characteristics; Chelating Agents; Complex; Cyclization; Detection; Devices; Diabetes Mellitus; Diagnosis; Diagnostic tests; Disease; Elements; Environment; Event; Excision; Extravasation; Goals; Health; Human; Hybrids; Hypertension; Immunodeficiency and Cancer; Immunologic Deficiency Syndromes; Ions; Ligands; Link; Magnesium; Measurement; Measures; Modeling; Modification; Modulus; Molecular; Molecular Target; Monitor; Morphology; Organism; Physiological; Plasma; Polymers; Preparation; Property; Regulation; Reporting; Research; Resistance; Role; Sampling; Serum; Signal Transduction; Specificity; Spectroscopy, Fourier Transform Infrared; Structure; Surface; System; Techniques; Technology; Testing; Titrations; Work; aqueous; base; biological systems; chelation; chemical property; design; experimental study; imprint; improved; molecular recognition; monomer; point of care; point-of-care diagnostics; polymerization; preference; pyridine; sensor; success; tool

PROJECT SUMMARY/ABSTRACT Magnesium (Mg2+) is an essential element found in all cells and is intimately involved in a myriad of biochemical processes and physiological functions. Improper regulation of Mg2+ in humans has been linked to diseases such as diabetes, hypertension, and immunodeficiency. Therefore, monitoring Mg2+ levels could aid in diagnosis and treatment of these disease states; however, current technologies for measurement of Mg2+ are not amenable to rapid, point-of-care analysis, and are limited by a need for extensive sample preparation or a lack of selectivity for Mg2+ over other cations. This proposal describes a hybrid carbon nanotube-molecularly imprinted polymer (CNT-MIP) chemiresistive sensor designed to selectively detect Mg2+. The proposed system consists of a CNT network modified with a functional enediyne that triggers the formation of a diradical upon binding Mg2+. In the presence of alkene- containing monomers, the diradical initiates a polymerization that propagates around the Mg2+-complexed template molecule. Removal of the Mg2+ template affords a polymer-coated CNT network with binding cavities that are expected to be highly selective for the recognition of Mg2+, and the binding event will be detected using conductometric measurements. Because the functional enediyne acts as both an initiator and an Mg2+-binding group, precise spatial control of the binding sites near the CNT network can be achieved while maintaining a direct connection from the binding site to the CNT. The MIP portion of the sensor is expected to provide the CNT network with structural robustness and resistance to deleterious bulk current leakage, which is normally problematic in aqueous CNT-based sensors.

项目摘要/摘要 镁（Mg 2+）是所有细胞中发现的必需元素，并且密切参与多种生物学功能。 生化过程和生理功能。人体中Mg 2+的不当调节与以下因素有关： 糖尿病、高血压和免疫缺陷等疾病。因此，监测Mg 2+水平可能有助于 在诊断和治疗这些疾病状态;然而，目前的技术用于测量Mg 2 + 不适于快速的即时分析，并且受到需要大量样品制备的限制 或相对于其它阳离子缺乏对Mg 2+的选择性。 该提议描述了一种杂化碳纳米管-分子印迹聚合物（CNT-MIP）化学电阻 设计用于选择性检测Mg 2+的传感器。所提出的系统由用碳纳米管改性的CNT网络组成， 功能性烯二炔，其在结合Mg 2+时触发双自由基的形成。在烯烃的存在下- 含有单体，双自由基引发聚合，该聚合围绕Mg 2+络合物传播。 模板分子除去Mg 2+模板提供了具有结合腔的聚合物涂覆的CNT网络 预期对于Mg 2+的识别具有高度选择性，并且结合事件将使用 电导测量。因为官能烯二炔既充当引发剂又充当Mg 2+结合剂， 组，可以实现CNT网络附近的结合位点的精确空间控制，同时保持 从结合位点到CNT的直接连接。预期传感器的MIP部分提供 CNT网络具有结构坚固性和对有害的体电流泄漏的抗性，其通常是 这在基于CNT的水性传感器中是有问题的。