Understanding the mechanism of genetic transformation

了解遗传转化的机制

• 批准号: 9817159
• 负责人: DAVID A DUBNAU
• 金额: $ 64.17万
• 依托单位: RBHS-NEW JERSEY MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-06-01 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9817159
• 关键词: ATP Hydrolysis; ATP phosphohydrolase; Address; Antibiotic Resistance; Architecture; Bacillus subtilis; Bacteria; Binding; Binding Proteins; Biochemistry; Carrier Proteins; Cell Wall; Cell membrane; Cell surface; Cells; Cellular biology; Collaborations; Competence; Complex; Coupled; Cryoelectron Microscopy; Cytoplasm; Cytoplasmic Protein; DNA; DNA Binding; DNA-Binding Proteins; Energy-Generating Resources; Evolution; Genes; Genetic; Genetic Transcription; Genetic Transformation; Goals; Gram-Negative Bacteria; Growth; Horizontal Gene Transfer; Immune Evasion; Laboratories; Mediating; Membrane; Methodology; Microscopy; Modeling; Molecular; Molecular Biology; Molecular Machines; Physiology; Pilum; Play; Process; Protein Biochemistry; Proteins; Proton-Motive Force; Role; Source; Stress; Structural Protein; Structure; System; Testing; Transmembrane Transport; Ursidae Family; Virulence; X-Ray Crystallography; expectation; experience; experimental study; helicase; insight; mutant; nuclease; optical traps; periplasm; protein complex; protein structure; protein transport; single molecule; structural biology; tool; translocase; uptake

Abstract Natural transformation in bacteria is an important mode of horizontal gene transfer. Generally, it contributes to bacterial evolution and specifically to the spread of antibiotic resistance and virulence genes. This proposal will use Bacillus subtilis as a model to address the fundamental, largely conserved mechanisms that enable the uptake of environmental DNA. It will address the binding of DNA to the surface of the cell, traversal through the cell wall and translocation of DNA across the cell membrane. This plan will provide broad insights into molecular details of transformation and will bring us closer to the long-term goal of understanding the mechanism of DNA uptake. It is proposed to investigate the energy sources for DNA uptake, the proteins involved, some of their structures and their interactions as components of a molecular machine. These aims will be pursued using the tools of cell biology, genetics, biochemistry and structural biology.

摘要