Proteomics of Primary Cilia through Proximity Labeling

通过邻近标记研究初级纤毛的蛋白质组学

• 批准号: 9590675
• 负责人: Maxence V Nachury
• 金额: $ 13.45万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-12-01 至 2019-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9590675
• 关键词: Biochemical; Biotin; Biotinylation; Catalogs; Cells; Cilia; Collection; Complex; Cystic kidney; DNA Sequence Alteration; Data; Defect; Development; Diffusion; Disease; Employee Strikes; Environment; Enzymes; Erinaceidae; Etiology; Functional disorder; G-Protein-Coupled Receptors; Genes; Genetic Transcription; Genotype; Goals; Hereditary Disease; Individual; Integral Membrane Protein; Interphase Cell; Isotope Labeling; Knowledge; Label; Lead; Mammalian Cell; Mass Spectrum Analysis; Membrane; Methods; Mitochondria; Molecular Analysis; Molecular Models; Mutation; Names; Obesity; Pathway interactions; Patients; Phosphatidylinositols; Phosphoric Monoester Hydrolases; Phosphotransferases; Pilot Projects; Proteins; Proteome; Proteomics; Receptor Signaling; Research; Retinal Degeneration; SHH gene; SSTR3 gene; Sensory; Signal Pathway; Signal Transduction; Signaling Molecule; Smell Perception; Somatostatin; Sorting - Cell Movement; Stimulus; Technology; Vision; base; ciliopathy; comparative; innovation; insight; malformation; method development; molecular modeling; mutant; novel; novel therapeutic intervention; public health relevance; receptor; response; skeletal; skeletal abnormality; small molecule; smoothened signaling pathway; somatostatin receptor 3; tool; trafficking

 DESCRIPTION (provided by applicant): Primary cilia organize signaling pathways such as vision, olfaction and the Hedgehog developmental pathway inside a specialized environment. The soluble contents and the membrane of the cilium are separated from the rest of the cell by diffusion barriers, thus endowing cilia with a unique physico-chemical environment. Depending on the status of Hedgehog signaling, specific signaling intermediates move into or out of cilia and it is predicted that the ciliary composition is modified by Hedgehog signaling. However, it has not been possible to assess the extent of the changes in the ciliary proteome upon Hedgehog pathway activation because cilia cannot be readily isolated from mammalian cells. Dysfunction of primary cilia is the underlying cause of a collection of hereditary disorders named ciliopathies characterized by obesity, kidney cysts, skeletal malformations and sensory defects. Obtaining a catalogue of primary cilium proteins and comparing the ciliary proteome of ciliopathy to healthy cells would provide a rapid and effective way to characterize ciliopathies. Thus, proteomics of primary cilia is currently a major technical blockage in the discovery of novel ciliary mechanisms. Rather than isolating cilia, we sought to attach the small molecule biotin to all ciliary protein by targeting a biotinylation enzyme to the primary cilium. This approach named proximity labeling has shown great promise in obtaining the proteome of mitochondria. We present promising preliminary data showing the validity of this approach for ciliary proteomics and wish to apply it to compare the ciliary proteome between different signaling states or between ciliopathy mutants and healthy cells. The results obtained by comparative ciliary proteomics will provide unique insights into several important questions and generate testable molecular models. The proposal is intended to develop a promising technology and to serve as a stepping-stone towards a rapid and effective comparative ciliary proteomics platform.

 描述（由申请人提供）：初级纤毛组织信号通路，如视觉，嗅觉和专门环境内的刺猬发育通路。纤毛的可溶性内容物和膜通过扩散屏障与细胞的其余部分隔开，从而赋予纤毛独特的物理化学环境。根据Hedgehog信号传导的状态，特定的信号传导中间体移入或移出纤毛，并且预测纤毛组成被Hedgehog信号传导修饰。然而，由于纤毛不能容易地从哺乳动物细胞中分离，因此不可能评估Hedgehog途径激活后纤毛蛋白质组的变化程度。 原发性纤毛功能障碍是一系列以肥胖、肾囊肿、骨骼畸形和感觉缺陷为特征的名为纤毛病的遗传性疾病的根本原因。获得初级纤毛蛋白的目录并将纤毛病的纤毛蛋白质组与健康细胞进行比较，将提供一种快速有效的方法来表征纤毛病。因此，初级纤毛的蛋白质组学是目前发现新的纤毛机制的主要技术障碍。 不是分离纤毛，我们试图通过将生物素化酶靶向初级纤毛来将小分子生物素附着到所有纤毛蛋白上。这种称为邻近标记的方法在获得线粒体蛋白质组方面显示出巨大的前景。我们提出了有前途的初步数据显示这种方法的有效性睫状体蛋白质组学，并希望将其应用于比较不同的信号状态或纤毛病变突变体和健康细胞之间的睫状体蛋白质组。 比较睫状体蛋白质组学获得的结果将为几个重要问题提供独特的见解，并产生可测试的分子模型。该提案旨在开发一种有前途的技术，并作为一个快速，有效的比较纤毛蛋白质组学平台的垫脚石。